Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Complex Structure of Lasiopodomys mandarinus vinogradovi Sex Chromosomes, Sex Determination, and Intraspecific Autosomal Polymorphism. / Romanenko, Svetlana A.; Smorkatcheva, Antonina V.; Kovalskaya, Yulia M. и др.
в: Genes, Том 11, № 4, 374, 04.2020.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Complex Structure of Lasiopodomys mandarinus vinogradovi Sex Chromosomes, Sex Determination, and Intraspecific Autosomal Polymorphism
AU - Romanenko, Svetlana A.
AU - Smorkatcheva, Antonina V.
AU - Kovalskaya, Yulia M.
AU - Prokopov, Dmitry Yu
AU - Lemskaya, Natalya A.
AU - Gladkikh, Olga L.
AU - Polikarpov, Ivan A.
AU - Serdyukova, Natalia A.
AU - Trifonov, Vladimir A.
AU - Molodtseva, Anna S.
AU - O'Brien, Patricia C.M.
AU - Golenishchev, Feodor N.
AU - Ferguson-Smith, Malcolm A.
AU - Graphodatsky, Alexander S.
PY - 2020/4
Y1 - 2020/4
N2 - The mandarin vole, Lasiopodomys mandarinus, is one of the most intriguing species among mammals with non-XX/XY sex chromosome system. It combines polymorphism in diploid chromosome numbers, variation in the morphology of autosomes, heteromorphism of X chromosomes, and several sex chromosome systems the origin of which remains unexplained. Here we elucidate the sex determination system in Lasiopodomys mandarinus vinogradovi using extensive karyotyping, crossbreeding experiments, molecular cytogenetic methods, and single chromosome DNA sequencing. Among 205 karyotyped voles, one male and three female combinations of sex chromosomes were revealed. The chromosome segregation pattern and karyomorph-related reproductive performances suggested an aberrant sex determination with almost half of the females carrying neo-X/neo-Y combination. The comparative chromosome painting strongly supported this proposition and revealed the mandarin vole sex chromosome systems originated due to at least two de novo autosomal translocations onto the ancestral X chromosome. The polymorphism in autosome 2 was not related to sex chromosome variability and was proved to result from pericentric inversions. Sequencing of microdissection derived of sex chromosomes allowed the determination of the coordinates for syntenic regions but did not reveal any Y-specific sequences. Several possible sex determination mechanisms as well as interpopulation karyological differences are discussed.
AB - The mandarin vole, Lasiopodomys mandarinus, is one of the most intriguing species among mammals with non-XX/XY sex chromosome system. It combines polymorphism in diploid chromosome numbers, variation in the morphology of autosomes, heteromorphism of X chromosomes, and several sex chromosome systems the origin of which remains unexplained. Here we elucidate the sex determination system in Lasiopodomys mandarinus vinogradovi using extensive karyotyping, crossbreeding experiments, molecular cytogenetic methods, and single chromosome DNA sequencing. Among 205 karyotyped voles, one male and three female combinations of sex chromosomes were revealed. The chromosome segregation pattern and karyomorph-related reproductive performances suggested an aberrant sex determination with almost half of the females carrying neo-X/neo-Y combination. The comparative chromosome painting strongly supported this proposition and revealed the mandarin vole sex chromosome systems originated due to at least two de novo autosomal translocations onto the ancestral X chromosome. The polymorphism in autosome 2 was not related to sex chromosome variability and was proved to result from pericentric inversions. Sequencing of microdissection derived of sex chromosomes allowed the determination of the coordinates for syntenic regions but did not reveal any Y-specific sequences. Several possible sex determination mechanisms as well as interpopulation karyological differences are discussed.
KW - aberrant sex determination
KW - chromosome painting
KW - comparative cytogenetics
KW - genome architecture
KW - high-throughput sequencing
KW - mandarin vole
KW - microdissection
KW - rearrangements
KW - rodents
KW - sex chromosomes
KW - KARYOTYPES
KW - SYSTEM
KW - MOUSE
KW - FEMALES
KW - DICROSTONYX-TORQUATUS
KW - EVOLUTION
KW - GENES
KW - VOLE
KW - DIVERSITY
KW - RODENTIA
UR - http://www.scopus.com/inward/record.url?scp=85082732050&partnerID=8YFLogxK
U2 - 10.3390/genes11040374
DO - 10.3390/genes11040374
M3 - Article
C2 - 32235544
AN - SCOPUS:85082732050
VL - 11
JO - Genes
JF - Genes
SN - 2073-4425
IS - 4
M1 - 374
ER -
ID: 23950743