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Complex Structure of Lasiopodomys mandarinus vinogradovi Sex Chromosomes, Sex Determination, and Intraspecific Autosomal Polymorphism. / Romanenko, Svetlana A.; Smorkatcheva, Antonina V.; Kovalskaya, Yulia M. et al.

In: Genes, Vol. 11, No. 4, 374, 04.2020.

Research output: Contribution to journal › Article › peer-review

Harvard

Romanenko, SA, Smorkatcheva, AV, Kovalskaya, YM, Prokopov, DY, Lemskaya, NA, Gladkikh, OL, Polikarpov, IA, Serdyukova, NA, Trifonov, VA, Molodtseva, AS, O'Brien, PCM, Golenishchev, FN, Ferguson-Smith, MA & Graphodatsky, AS 2020, 'Complex Structure of Lasiopodomys mandarinus vinogradovi Sex Chromosomes, Sex Determination, and Intraspecific Autosomal Polymorphism', Genes, vol. 11, no. 4, 374. https://doi.org/10.3390/genes11040374

APA

Romanenko, S. A., Smorkatcheva, A. V., Kovalskaya, Y. M., Prokopov, D. Y., Lemskaya, N. A., Gladkikh, O. L., Polikarpov, I. A., Serdyukova, N. A., Trifonov, V. A., Molodtseva, A. S., O'Brien, P. C. M., Golenishchev, F. N., Ferguson-Smith, M. A., & Graphodatsky, A. S. (2020). Complex Structure of Lasiopodomys mandarinus vinogradovi Sex Chromosomes, Sex Determination, and Intraspecific Autosomal Polymorphism. Genes, 11(4), [374]. https://doi.org/10.3390/genes11040374

Vancouver

Romanenko SA, Smorkatcheva AV, Kovalskaya YM, Prokopov DY, Lemskaya NA, Gladkikh OL et al. Complex Structure of Lasiopodomys mandarinus vinogradovi Sex Chromosomes, Sex Determination, and Intraspecific Autosomal Polymorphism. Genes. 2020 Apr;11(4):374. doi: 10.3390/genes11040374

Author

Romanenko, Svetlana A. ; Smorkatcheva, Antonina V. ; Kovalskaya, Yulia M. et al. / Complex Structure of Lasiopodomys mandarinus vinogradovi Sex Chromosomes, Sex Determination, and Intraspecific Autosomal Polymorphism. In: Genes. 2020 ; Vol. 11, No. 4.

BibTeX

@article{41023774f9214e28b39b0977d11e55c9,
title = "Complex Structure of Lasiopodomys mandarinus vinogradovi Sex Chromosomes, Sex Determination, and Intraspecific Autosomal Polymorphism",
abstract = "The mandarin vole, Lasiopodomys mandarinus, is one of the most intriguing species among mammals with non-XX/XY sex chromosome system. It combines polymorphism in diploid chromosome numbers, variation in the morphology of autosomes, heteromorphism of X chromosomes, and several sex chromosome systems the origin of which remains unexplained. Here we elucidate the sex determination system in Lasiopodomys mandarinus vinogradovi using extensive karyotyping, crossbreeding experiments, molecular cytogenetic methods, and single chromosome DNA sequencing. Among 205 karyotyped voles, one male and three female combinations of sex chromosomes were revealed. The chromosome segregation pattern and karyomorph-related reproductive performances suggested an aberrant sex determination with almost half of the females carrying neo-X/neo-Y combination. The comparative chromosome painting strongly supported this proposition and revealed the mandarin vole sex chromosome systems originated due to at least two de novo autosomal translocations onto the ancestral X chromosome. The polymorphism in autosome 2 was not related to sex chromosome variability and was proved to result from pericentric inversions. Sequencing of microdissection derived of sex chromosomes allowed the determination of the coordinates for syntenic regions but did not reveal any Y-specific sequences. Several possible sex determination mechanisms as well as interpopulation karyological differences are discussed.",
keywords = "aberrant sex determination, chromosome painting, comparative cytogenetics, genome architecture, high-throughput sequencing, mandarin vole, microdissection, rearrangements, rodents, sex chromosomes, KARYOTYPES, SYSTEM, MOUSE, FEMALES, DICROSTONYX-TORQUATUS, EVOLUTION, GENES, VOLE, DIVERSITY, RODENTIA",
author = "Romanenko, {Svetlana A.} and Smorkatcheva, {Antonina V.} and Kovalskaya, {Yulia M.} and Prokopov, {Dmitry Yu} and Lemskaya, {Natalya A.} and Gladkikh, {Olga L.} and Polikarpov, {Ivan A.} and Serdyukova, {Natalia A.} and Trifonov, {Vladimir A.} and Molodtseva, {Anna S.} and O'Brien, {Patricia C.M.} and Golenishchev, {Feodor N.} and Ferguson-Smith, {Malcolm A.} and Graphodatsky, {Alexander S.}",
year = "2020",
month = apr,
doi = "10.3390/genes11040374",
language = "English",
volume = "11",
journal = "Genes",
issn = "2073-4425",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "4",

}

RIS

TY - JOUR

T1 - Complex Structure of Lasiopodomys mandarinus vinogradovi Sex Chromosomes, Sex Determination, and Intraspecific Autosomal Polymorphism

AU - Romanenko, Svetlana A.

AU - Smorkatcheva, Antonina V.

AU - Kovalskaya, Yulia M.

AU - Prokopov, Dmitry Yu

AU - Lemskaya, Natalya A.

AU - Gladkikh, Olga L.

AU - Polikarpov, Ivan A.

AU - Serdyukova, Natalia A.

AU - Trifonov, Vladimir A.

AU - Molodtseva, Anna S.

AU - O'Brien, Patricia C.M.

AU - Golenishchev, Feodor N.

AU - Ferguson-Smith, Malcolm A.

AU - Graphodatsky, Alexander S.

PY - 2020/4

Y1 - 2020/4

N2 - The mandarin vole, Lasiopodomys mandarinus, is one of the most intriguing species among mammals with non-XX/XY sex chromosome system. It combines polymorphism in diploid chromosome numbers, variation in the morphology of autosomes, heteromorphism of X chromosomes, and several sex chromosome systems the origin of which remains unexplained. Here we elucidate the sex determination system in Lasiopodomys mandarinus vinogradovi using extensive karyotyping, crossbreeding experiments, molecular cytogenetic methods, and single chromosome DNA sequencing. Among 205 karyotyped voles, one male and three female combinations of sex chromosomes were revealed. The chromosome segregation pattern and karyomorph-related reproductive performances suggested an aberrant sex determination with almost half of the females carrying neo-X/neo-Y combination. The comparative chromosome painting strongly supported this proposition and revealed the mandarin vole sex chromosome systems originated due to at least two de novo autosomal translocations onto the ancestral X chromosome. The polymorphism in autosome 2 was not related to sex chromosome variability and was proved to result from pericentric inversions. Sequencing of microdissection derived of sex chromosomes allowed the determination of the coordinates for syntenic regions but did not reveal any Y-specific sequences. Several possible sex determination mechanisms as well as interpopulation karyological differences are discussed.

AB - The mandarin vole, Lasiopodomys mandarinus, is one of the most intriguing species among mammals with non-XX/XY sex chromosome system. It combines polymorphism in diploid chromosome numbers, variation in the morphology of autosomes, heteromorphism of X chromosomes, and several sex chromosome systems the origin of which remains unexplained. Here we elucidate the sex determination system in Lasiopodomys mandarinus vinogradovi using extensive karyotyping, crossbreeding experiments, molecular cytogenetic methods, and single chromosome DNA sequencing. Among 205 karyotyped voles, one male and three female combinations of sex chromosomes were revealed. The chromosome segregation pattern and karyomorph-related reproductive performances suggested an aberrant sex determination with almost half of the females carrying neo-X/neo-Y combination. The comparative chromosome painting strongly supported this proposition and revealed the mandarin vole sex chromosome systems originated due to at least two de novo autosomal translocations onto the ancestral X chromosome. The polymorphism in autosome 2 was not related to sex chromosome variability and was proved to result from pericentric inversions. Sequencing of microdissection derived of sex chromosomes allowed the determination of the coordinates for syntenic regions but did not reveal any Y-specific sequences. Several possible sex determination mechanisms as well as interpopulation karyological differences are discussed.

KW - aberrant sex determination

KW - chromosome painting

KW - comparative cytogenetics

KW - genome architecture

KW - high-throughput sequencing

KW - mandarin vole

KW - microdissection

KW - rearrangements

KW - rodents

KW - sex chromosomes

KW - KARYOTYPES

KW - SYSTEM

KW - MOUSE

KW - FEMALES

KW - DICROSTONYX-TORQUATUS

KW - EVOLUTION

KW - GENES

KW - VOLE

KW - DIVERSITY

KW - RODENTIA

UR - http://www.scopus.com/inward/record.url?scp=85082732050&partnerID=8YFLogxK

U2 - 10.3390/genes11040374

DO - 10.3390/genes11040374

M3 - Article

C2 - 32235544

AN - SCOPUS:85082732050

VL - 11

JO - Genes

JF - Genes

SN - 2073-4425

IS - 4

M1 - 374

ER -

ID: 23950743