TY - JOUR

T1 - Changes in the number and morphology of blood cells in mice pretreated with RNA preparations and exposed to 8 Gy of gamma radiation

AU - Dubatolova, T. D.

AU - Ritter, G. S.

AU - Proskurina, A. S.

AU - Kisaretova, P. E.

AU - Nikolin, V. P.

AU - Popova, N. A.

AU - Ruzanova, V. S.

AU - Taranov, O. S.

AU - Kolchanov, N. A.

AU - Bogachev, S. S.

N1 - Funding Information: This work was supported by the Russian Ministry of Science and High Education, State Budgeted Project No. FWNR-2022-0016 for the Institute of Cytology and Genetics, Novosibirsk. Microscopic analysis was supported by the Russian Ministry of Science and High Education, State Budgeted Project No. FWGZ-2021-0017 for the Institute of Molecular and Cellular Biology, Novosibirsk. Publisher Copyright: © 2022 Novin Medical Radiation Institute. All rights reserved.

PY - 2022

Y1 - 2022

N2 - Background: In our recent studies, yeast double-stranded RNA showed radioprotective effect in mice exposed to 9.4 Gy of gamma radiation. The current work continues our inquiry and describes the changes in the number and morphology of blood cells in mice injected with double-stranded RNA from Saccharomyces cerevisiae prior to gamma irradiation. To be capable of estimating the required parameters, we have used 8 Gy of radiation, which allowed mice to survive for up to 37 days (LD60/30). Materials and Methods: Animals received single intravenous injections of one of the following compounds: (1) 7 mg of total RNA isolated from baking yeast Saccharomyces cerevisiae, (2) 200 μg of double-stranded RNA or (3) 400 μg of double-stranded RNA. 30 minutes later, using a 137Cs gamma emitter, mice were irradiated with a dose of 8 Gy at the rate of 1.4 Gy/min. The radioprotective effect of preparations was assessed based on death rates. Results: Injections of both 200 μg of double-stranded RNA and 7 mg of total RNA prior to irradiation provided the best radioprotective effects, ensuring the survival of 100% of animals. Changes in the number of blood cells and their morphological aberrations were being monitored for 37 days after irradiation. Conclusion: The protection from lethal radiation doses is associated with the preservation and rapid recovery of leukocytic and erythroid lineages.

AB - Background: In our recent studies, yeast double-stranded RNA showed radioprotective effect in mice exposed to 9.4 Gy of gamma radiation. The current work continues our inquiry and describes the changes in the number and morphology of blood cells in mice injected with double-stranded RNA from Saccharomyces cerevisiae prior to gamma irradiation. To be capable of estimating the required parameters, we have used 8 Gy of radiation, which allowed mice to survive for up to 37 days (LD60/30). Materials and Methods: Animals received single intravenous injections of one of the following compounds: (1) 7 mg of total RNA isolated from baking yeast Saccharomyces cerevisiae, (2) 200 μg of double-stranded RNA or (3) 400 μg of double-stranded RNA. 30 minutes later, using a 137Cs gamma emitter, mice were irradiated with a dose of 8 Gy at the rate of 1.4 Gy/min. The radioprotective effect of preparations was assessed based on death rates. Results: Injections of both 200 μg of double-stranded RNA and 7 mg of total RNA prior to irradiation provided the best radioprotective effects, ensuring the survival of 100% of animals. Changes in the number of blood cells and their morphological aberrations were being monitored for 37 days after irradiation. Conclusion: The protection from lethal radiation doses is associated with the preservation and rapid recovery of leukocytic and erythroid lineages.

KW - Double-stranded RNA

KW - lymphocytes

KW - monocytes

KW - mutagenic activity

KW - neutrophils

UR - http://www.scopus.com/inward/record.url?scp=85134167831&partnerID=8YFLogxK

U2 - 10.52547/ijrr.20.2.16

DO - 10.52547/ijrr.20.2.16

M3 - Article

AN - SCOPUS:85134167831

VL - 20

SP - 361

EP - 368

JO - International Journal of Radiation Research

JF - International Journal of Radiation Research

SN - 2322-3243

IS - 2

ER -