Research output: Contribution to journal › Article › peer-review
Changes in the number and morphology of blood cells in mice pretreated with RNA preparations and exposed to 8 Gy of gamma radiation. / Dubatolova, T. D.; Ritter, G. S.; Proskurina, A. S. et al.
In: International Journal of Radiation Research, Vol. 20, No. 2, 2022, p. 361-368.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Changes in the number and morphology of blood cells in mice pretreated with RNA preparations and exposed to 8 Gy of gamma radiation
AU - Dubatolova, T. D.
AU - Ritter, G. S.
AU - Proskurina, A. S.
AU - Kisaretova, P. E.
AU - Nikolin, V. P.
AU - Popova, N. A.
AU - Ruzanova, V. S.
AU - Taranov, O. S.
AU - Kolchanov, N. A.
AU - Bogachev, S. S.
N1 - Funding Information: This work was supported by the Russian Ministry of Science and High Education, State Budgeted Project No. FWNR-2022-0016 for the Institute of Cytology and Genetics, Novosibirsk. Microscopic analysis was supported by the Russian Ministry of Science and High Education, State Budgeted Project No. FWGZ-2021-0017 for the Institute of Molecular and Cellular Biology, Novosibirsk. Publisher Copyright: © 2022 Novin Medical Radiation Institute. All rights reserved.
PY - 2022
Y1 - 2022
N2 - Background: In our recent studies, yeast double-stranded RNA showed radioprotective effect in mice exposed to 9.4 Gy of gamma radiation. The current work continues our inquiry and describes the changes in the number and morphology of blood cells in mice injected with double-stranded RNA from Saccharomyces cerevisiae prior to gamma irradiation. To be capable of estimating the required parameters, we have used 8 Gy of radiation, which allowed mice to survive for up to 37 days (LD60/30). Materials and Methods: Animals received single intravenous injections of one of the following compounds: (1) 7 mg of total RNA isolated from baking yeast Saccharomyces cerevisiae, (2) 200 μg of double-stranded RNA or (3) 400 μg of double-stranded RNA. 30 minutes later, using a 137Cs gamma emitter, mice were irradiated with a dose of 8 Gy at the rate of 1.4 Gy/min. The radioprotective effect of preparations was assessed based on death rates. Results: Injections of both 200 μg of double-stranded RNA and 7 mg of total RNA prior to irradiation provided the best radioprotective effects, ensuring the survival of 100% of animals. Changes in the number of blood cells and their morphological aberrations were being monitored for 37 days after irradiation. Conclusion: The protection from lethal radiation doses is associated with the preservation and rapid recovery of leukocytic and erythroid lineages.
AB - Background: In our recent studies, yeast double-stranded RNA showed radioprotective effect in mice exposed to 9.4 Gy of gamma radiation. The current work continues our inquiry and describes the changes in the number and morphology of blood cells in mice injected with double-stranded RNA from Saccharomyces cerevisiae prior to gamma irradiation. To be capable of estimating the required parameters, we have used 8 Gy of radiation, which allowed mice to survive for up to 37 days (LD60/30). Materials and Methods: Animals received single intravenous injections of one of the following compounds: (1) 7 mg of total RNA isolated from baking yeast Saccharomyces cerevisiae, (2) 200 μg of double-stranded RNA or (3) 400 μg of double-stranded RNA. 30 minutes later, using a 137Cs gamma emitter, mice were irradiated with a dose of 8 Gy at the rate of 1.4 Gy/min. The radioprotective effect of preparations was assessed based on death rates. Results: Injections of both 200 μg of double-stranded RNA and 7 mg of total RNA prior to irradiation provided the best radioprotective effects, ensuring the survival of 100% of animals. Changes in the number of blood cells and their morphological aberrations were being monitored for 37 days after irradiation. Conclusion: The protection from lethal radiation doses is associated with the preservation and rapid recovery of leukocytic and erythroid lineages.
KW - Double-stranded RNA
KW - lymphocytes
KW - monocytes
KW - mutagenic activity
KW - neutrophils
UR - http://www.scopus.com/inward/record.url?scp=85134167831&partnerID=8YFLogxK
U2 - 10.52547/ijrr.20.2.16
DO - 10.52547/ijrr.20.2.16
M3 - Article
AN - SCOPUS:85134167831
VL - 20
SP - 361
EP - 368
JO - International Journal of Radiation Research
JF - International Journal of Radiation Research
SN - 2322-3243
IS - 2
ER -
ID: 36716585