TY - JOUR

T1 - Changes in haematopoietic progenitor colony differentiation and proliferation and the production of different abzymes in EAE mice treated with DNA

AU - Aulova, Kseniya S.

AU - Toporkova, Ludmila B.

AU - Lopatnikova, Julia A.

AU - Alshevskaya, Alina A.

AU - Sennikov, Sergei V.

AU - Buneva, Valentina N.

AU - Budde, Thomas

AU - Meuth, Sven G.

AU - Popova, Nelly A.

AU - Orlovskaya, Irina A.

AU - Nevinsky, Georgy A.

N1 - Publisher Copyright: © 2017 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.

PY - 2017/12/1

Y1 - 2017/12/1

N2 - Immunization of experimental autoimmune encephalomyelitis (EAE)-prone C57BL/6 mice with MOG35-55 (a model used to study aspects of human multiple sclerosis) is known to lead to the production of various abzymes. The production of catalytic IgGs that can efficiently hydrolyse myelin basic protein (MBP), MOG and DNA is associated with changes in the profile of differentiation and level of proliferation of mice bone marrow haematopoietic stem cells (HSCs). As MOG simulates the production of abzymes with high DNase activity, we compared the effects of DNA and MOG immunization on EAE-prone mice. In contrast to MOG, immunization with DNA leads to a suppression of proteinuria, a decrease in the concentrations of antibodies to MOG and DNA and a reduction in abzyme production. Immunization with DNA only resulted in a significant increase in DNase activity over 40 days where it became 122-fold higher than before immunization, and fivefold higher when comparing to the maximal activity obtained after MOG treatment. DNA and MOG immunization had different effects on the differentiation profiles of HSCs, lymphocyte proliferation, and the level of apoptosis in bone marrow and other organs of mice. The data indicate that for C57BL/6 mice, DNA may have antagonistic effects with respect to MOG immunization. The usually fast immune response following MOG injection in C57BL/6 mice is strongly delayed after immunization with DNA, which is probably due to a rearrangement of the immune system following the response to DNA.

AB - Immunization of experimental autoimmune encephalomyelitis (EAE)-prone C57BL/6 mice with MOG35-55 (a model used to study aspects of human multiple sclerosis) is known to lead to the production of various abzymes. The production of catalytic IgGs that can efficiently hydrolyse myelin basic protein (MBP), MOG and DNA is associated with changes in the profile of differentiation and level of proliferation of mice bone marrow haematopoietic stem cells (HSCs). As MOG simulates the production of abzymes with high DNase activity, we compared the effects of DNA and MOG immunization on EAE-prone mice. In contrast to MOG, immunization with DNA leads to a suppression of proteinuria, a decrease in the concentrations of antibodies to MOG and DNA and a reduction in abzyme production. Immunization with DNA only resulted in a significant increase in DNase activity over 40 days where it became 122-fold higher than before immunization, and fivefold higher when comparing to the maximal activity obtained after MOG treatment. DNA and MOG immunization had different effects on the differentiation profiles of HSCs, lymphocyte proliferation, and the level of apoptosis in bone marrow and other organs of mice. The data indicate that for C57BL/6 mice, DNA may have antagonistic effects with respect to MOG immunization. The usually fast immune response following MOG injection in C57BL/6 mice is strongly delayed after immunization with DNA, which is probably due to a rearrangement of the immune system following the response to DNA.

KW - C57BL/6 mice

KW - Catalytic antibodies

KW - Colony formation

KW - EAE model

KW - Haematopoietic progenitor differentiation

KW - Immunization with DNA

KW - haematopoietic progenitor differentiation

KW - immunization with DNA

KW - catalytic antibodies

KW - colony formation

KW - Antibodies, Catalytic/biosynthesis

KW - Encephalomyelitis, Autoimmune, Experimental/chemically induced

KW - Myelin-Oligodendrocyte Glycoprotein/administration & dosage

KW - Cell Proliferation

KW - Mice, Inbred C57BL

KW - Immunization/methods

KW - Peptide Fragments/administration & dosage

KW - Hematopoietic Stem Cells/cytology

KW - DNA/administration & dosage

KW - Animals

KW - Immunity, Humoral

KW - Cell Differentiation

KW - Colony-Forming Units Assay

KW - Mice

KW - AUTOANTIBODIES

KW - MRL/MPJ-LPR MICE

KW - B-CELLS

KW - MYELIN BASIC-PROTEIN

KW - HYDROLYZING ANTIBODIES

KW - MULTIPLE-SCLEROSIS

KW - CATALYTIC ANTIBODIES

KW - SERA

KW - AUTOIMMUNE

KW - DISEASES

UR - http://www.scopus.com/inward/record.url?scp=85026776267&partnerID=8YFLogxK

U2 - 10.1111/jcmm.13289

DO - 10.1111/jcmm.13289

M3 - Article

C2 - 28780774

AN - SCOPUS:85026776267

VL - 21

SP - 3795

EP - 3809

JO - Journal of Cellular and Molecular Medicine

JF - Journal of Cellular and Molecular Medicine

SN - 1582-1838

IS - 12

ER -