Research output: Contribution to journal › Article › peer-review
Changes in haematopoietic progenitor colony differentiation and proliferation and the production of different abzymes in EAE mice treated with DNA. / Aulova, Kseniya S.; Toporkova, Ludmila B.; Lopatnikova, Julia A. et al.
In: Journal of Cellular and Molecular Medicine, Vol. 21, No. 12, 01.12.2017, p. 3795-3809.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Changes in haematopoietic progenitor colony differentiation and proliferation and the production of different abzymes in EAE mice treated with DNA
AU - Aulova, Kseniya S.
AU - Toporkova, Ludmila B.
AU - Lopatnikova, Julia A.
AU - Alshevskaya, Alina A.
AU - Sennikov, Sergei V.
AU - Buneva, Valentina N.
AU - Budde, Thomas
AU - Meuth, Sven G.
AU - Popova, Nelly A.
AU - Orlovskaya, Irina A.
AU - Nevinsky, Georgy A.
N1 - Publisher Copyright: © 2017 The Authors. Journal of Cellular and Molecular Medicine published by John Wiley & Sons Ltd and Foundation for Cellular and Molecular Medicine.
PY - 2017/12/1
Y1 - 2017/12/1
N2 - Immunization of experimental autoimmune encephalomyelitis (EAE)-prone C57BL/6 mice with MOG35-55 (a model used to study aspects of human multiple sclerosis) is known to lead to the production of various abzymes. The production of catalytic IgGs that can efficiently hydrolyse myelin basic protein (MBP), MOG and DNA is associated with changes in the profile of differentiation and level of proliferation of mice bone marrow haematopoietic stem cells (HSCs). As MOG simulates the production of abzymes with high DNase activity, we compared the effects of DNA and MOG immunization on EAE-prone mice. In contrast to MOG, immunization with DNA leads to a suppression of proteinuria, a decrease in the concentrations of antibodies to MOG and DNA and a reduction in abzyme production. Immunization with DNA only resulted in a significant increase in DNase activity over 40 days where it became 122-fold higher than before immunization, and fivefold higher when comparing to the maximal activity obtained after MOG treatment. DNA and MOG immunization had different effects on the differentiation profiles of HSCs, lymphocyte proliferation, and the level of apoptosis in bone marrow and other organs of mice. The data indicate that for C57BL/6 mice, DNA may have antagonistic effects with respect to MOG immunization. The usually fast immune response following MOG injection in C57BL/6 mice is strongly delayed after immunization with DNA, which is probably due to a rearrangement of the immune system following the response to DNA.
AB - Immunization of experimental autoimmune encephalomyelitis (EAE)-prone C57BL/6 mice with MOG35-55 (a model used to study aspects of human multiple sclerosis) is known to lead to the production of various abzymes. The production of catalytic IgGs that can efficiently hydrolyse myelin basic protein (MBP), MOG and DNA is associated with changes in the profile of differentiation and level of proliferation of mice bone marrow haematopoietic stem cells (HSCs). As MOG simulates the production of abzymes with high DNase activity, we compared the effects of DNA and MOG immunization on EAE-prone mice. In contrast to MOG, immunization with DNA leads to a suppression of proteinuria, a decrease in the concentrations of antibodies to MOG and DNA and a reduction in abzyme production. Immunization with DNA only resulted in a significant increase in DNase activity over 40 days where it became 122-fold higher than before immunization, and fivefold higher when comparing to the maximal activity obtained after MOG treatment. DNA and MOG immunization had different effects on the differentiation profiles of HSCs, lymphocyte proliferation, and the level of apoptosis in bone marrow and other organs of mice. The data indicate that for C57BL/6 mice, DNA may have antagonistic effects with respect to MOG immunization. The usually fast immune response following MOG injection in C57BL/6 mice is strongly delayed after immunization with DNA, which is probably due to a rearrangement of the immune system following the response to DNA.
KW - C57BL/6 mice
KW - Catalytic antibodies
KW - Colony formation
KW - EAE model
KW - Haematopoietic progenitor differentiation
KW - Immunization with DNA
KW - haematopoietic progenitor differentiation
KW - immunization with DNA
KW - catalytic antibodies
KW - colony formation
KW - Antibodies, Catalytic/biosynthesis
KW - Encephalomyelitis, Autoimmune, Experimental/chemically induced
KW - Myelin-Oligodendrocyte Glycoprotein/administration & dosage
KW - Cell Proliferation
KW - Mice, Inbred C57BL
KW - Immunization/methods
KW - Peptide Fragments/administration & dosage
KW - Hematopoietic Stem Cells/cytology
KW - DNA/administration & dosage
KW - Animals
KW - Immunity, Humoral
KW - Cell Differentiation
KW - Colony-Forming Units Assay
KW - Mice
KW - AUTOANTIBODIES
KW - MRL/MPJ-LPR MICE
KW - B-CELLS
KW - MYELIN BASIC-PROTEIN
KW - HYDROLYZING ANTIBODIES
KW - MULTIPLE-SCLEROSIS
KW - CATALYTIC ANTIBODIES
KW - SERA
KW - AUTOIMMUNE
KW - DISEASES
UR - http://www.scopus.com/inward/record.url?scp=85026776267&partnerID=8YFLogxK
U2 - 10.1111/jcmm.13289
DO - 10.1111/jcmm.13289
M3 - Article
C2 - 28780774
AN - SCOPUS:85026776267
VL - 21
SP - 3795
EP - 3809
JO - Journal of Cellular and Molecular Medicine
JF - Journal of Cellular and Molecular Medicine
SN - 1582-1838
IS - 12
ER -
ID: 8676735