TY - JOUR

T1 - Cervicovaginal-Microbiome Analysis by 16S Sequencing and Real-Time PCR in Patients from Novosibirsk (Russia) with Cervical Lesions and Several Years after Cancer Treatment

AU - Ivanov, Mikhail K

AU - Brenner, Evgeny V

AU - Hodkevich, Anastasia A

AU - Dzyubenko, Victoria V

AU - Krasilnikov, Sergey E

AU - Mansurova, Alphiya S

AU - Vakhturova, Irina E

AU - Agletdinov, Eduard F

AU - Shumeikina, Anastasia O

AU - Chernyshova, Alyona L

AU - Titov, Sergei E

N1 - Funding: This research was funded by the Russian Science Foundation, project No. 20-14-00074, and by the Fundamental Scientific Research Program, project No. FWGZ-2021-0014.

PY - 2023/1/1

Y1 - 2023/1/1

N2 - Disturbed cervicovaginal-microbiome (CVM) structure promotes human papillomavirus (HPV) persistence and reflects risks of cervical lesions and cancer onset and recurrence. Therefore, microbiomic biomarkers may be useful for cervical disease screening and patient management. Here, by 16S rRNA gene sequencing and commercial PCR-based diagnostic kits, we profiled CVM in cytological preparations from 140 HPV-tested women (from Novosibirsk, Russia) with normal cytological findings, cervical lesions, or cancer and from 101 women who had recently received different cancer therapies. An increase in lesion severity was accompanied by higher HPV prevalence and elevated CVM biodiversity. Post-treatment CVM was found to be enriched with well-known microbial biomarkers of dysbiosis, just as in cervical disease. Nonetheless, concentrations of some skin-borne and environmental species (which gradually increased with increasing lesion severity)-especially Cutibacterium spp., Achromobacter spp., and Ralstonia pickettii-was low in post-treatment patients and depended on treatment types. Frequency of Lactobacillus iners dominance was high in all groups and depended on treatment types in post-treatment patients. Microbiome analysis via PCR-based kits revealed statistically significant differences among all groups of patients. Thus, microbiome profiling may help to find diagnostic and prognostic markers for management of cervical lesions; quantitative PCR-based kits may be suitable for these purposes.

AB - Disturbed cervicovaginal-microbiome (CVM) structure promotes human papillomavirus (HPV) persistence and reflects risks of cervical lesions and cancer onset and recurrence. Therefore, microbiomic biomarkers may be useful for cervical disease screening and patient management. Here, by 16S rRNA gene sequencing and commercial PCR-based diagnostic kits, we profiled CVM in cytological preparations from 140 HPV-tested women (from Novosibirsk, Russia) with normal cytological findings, cervical lesions, or cancer and from 101 women who had recently received different cancer therapies. An increase in lesion severity was accompanied by higher HPV prevalence and elevated CVM biodiversity. Post-treatment CVM was found to be enriched with well-known microbial biomarkers of dysbiosis, just as in cervical disease. Nonetheless, concentrations of some skin-borne and environmental species (which gradually increased with increasing lesion severity)-especially Cutibacterium spp., Achromobacter spp., and Ralstonia pickettii-was low in post-treatment patients and depended on treatment types. Frequency of Lactobacillus iners dominance was high in all groups and depended on treatment types in post-treatment patients. Microbiome analysis via PCR-based kits revealed statistically significant differences among all groups of patients. Thus, microbiome profiling may help to find diagnostic and prognostic markers for management of cervical lesions; quantitative PCR-based kits may be suitable for these purposes.

KW - 16S RRNA GENE SEQUENCING

KW - CUTIBACTERIUM ACNES

KW - LACTOBACILLUS INERS

KW - CANCER TREATMENT

KW - CERVICAL CANCER

KW - CERVICOVAGINAL MICROBIOME

KW - HIGH-GRADE SQUAMOUS INTRAEPITHELIAL LESION

KW - HUMAN PAPILLOMAVIRUS

KW - POST-TREATMENT EFFECT

KW - REAL-TIME PCR

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85145898176&origin=inward&txGid=3f592dba0c1f662d6d9ce6cdb94795ea

UR - https://elibrary.ru/item.asp?id=50101709

U2 - 10.3390/diagnostics13010140

DO - 10.3390/diagnostics13010140

M3 - Article

C2 - 36611432

VL - 13

JO - Diagnostics

JF - Diagnostics

SN - 2075-4418

IS - 1

M1 - 140

ER -