Research output: Contribution to journal › Article › peer-review
Cervicovaginal-Microbiome Analysis by 16S Sequencing and Real-Time PCR in Patients from Novosibirsk (Russia) with Cervical Lesions and Several Years after Cancer Treatment. / Ivanov, Mikhail K; Brenner, Evgeny V; Hodkevich, Anastasia A et al.
In: Diagnostics, Vol. 13, No. 1, 140, 01.01.2023.Research output: Contribution to journal › Article › peer-review
}
TY - JOUR
T1 - Cervicovaginal-Microbiome Analysis by 16S Sequencing and Real-Time PCR in Patients from Novosibirsk (Russia) with Cervical Lesions and Several Years after Cancer Treatment
AU - Ivanov, Mikhail K
AU - Brenner, Evgeny V
AU - Hodkevich, Anastasia A
AU - Dzyubenko, Victoria V
AU - Krasilnikov, Sergey E
AU - Mansurova, Alphiya S
AU - Vakhturova, Irina E
AU - Agletdinov, Eduard F
AU - Shumeikina, Anastasia O
AU - Chernyshova, Alyona L
AU - Titov, Sergei E
N1 - Funding: This research was funded by the Russian Science Foundation, project No. 20-14-00074, and by the Fundamental Scientific Research Program, project No. FWGZ-2021-0014.
PY - 2023/1/1
Y1 - 2023/1/1
N2 - Disturbed cervicovaginal-microbiome (CVM) structure promotes human papillomavirus (HPV) persistence and reflects risks of cervical lesions and cancer onset and recurrence. Therefore, microbiomic biomarkers may be useful for cervical disease screening and patient management. Here, by 16S rRNA gene sequencing and commercial PCR-based diagnostic kits, we profiled CVM in cytological preparations from 140 HPV-tested women (from Novosibirsk, Russia) with normal cytological findings, cervical lesions, or cancer and from 101 women who had recently received different cancer therapies. An increase in lesion severity was accompanied by higher HPV prevalence and elevated CVM biodiversity. Post-treatment CVM was found to be enriched with well-known microbial biomarkers of dysbiosis, just as in cervical disease. Nonetheless, concentrations of some skin-borne and environmental species (which gradually increased with increasing lesion severity)-especially Cutibacterium spp., Achromobacter spp., and Ralstonia pickettii-was low in post-treatment patients and depended on treatment types. Frequency of Lactobacillus iners dominance was high in all groups and depended on treatment types in post-treatment patients. Microbiome analysis via PCR-based kits revealed statistically significant differences among all groups of patients. Thus, microbiome profiling may help to find diagnostic and prognostic markers for management of cervical lesions; quantitative PCR-based kits may be suitable for these purposes.
AB - Disturbed cervicovaginal-microbiome (CVM) structure promotes human papillomavirus (HPV) persistence and reflects risks of cervical lesions and cancer onset and recurrence. Therefore, microbiomic biomarkers may be useful for cervical disease screening and patient management. Here, by 16S rRNA gene sequencing and commercial PCR-based diagnostic kits, we profiled CVM in cytological preparations from 140 HPV-tested women (from Novosibirsk, Russia) with normal cytological findings, cervical lesions, or cancer and from 101 women who had recently received different cancer therapies. An increase in lesion severity was accompanied by higher HPV prevalence and elevated CVM biodiversity. Post-treatment CVM was found to be enriched with well-known microbial biomarkers of dysbiosis, just as in cervical disease. Nonetheless, concentrations of some skin-borne and environmental species (which gradually increased with increasing lesion severity)-especially Cutibacterium spp., Achromobacter spp., and Ralstonia pickettii-was low in post-treatment patients and depended on treatment types. Frequency of Lactobacillus iners dominance was high in all groups and depended on treatment types in post-treatment patients. Microbiome analysis via PCR-based kits revealed statistically significant differences among all groups of patients. Thus, microbiome profiling may help to find diagnostic and prognostic markers for management of cervical lesions; quantitative PCR-based kits may be suitable for these purposes.
KW - 16S RRNA GENE SEQUENCING
KW - CUTIBACTERIUM ACNES
KW - LACTOBACILLUS INERS
KW - CANCER TREATMENT
KW - CERVICAL CANCER
KW - CERVICOVAGINAL MICROBIOME
KW - HIGH-GRADE SQUAMOUS INTRAEPITHELIAL LESION
KW - HUMAN PAPILLOMAVIRUS
KW - POST-TREATMENT EFFECT
KW - REAL-TIME PCR
UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85145898176&origin=inward&txGid=3f592dba0c1f662d6d9ce6cdb94795ea
UR - https://elibrary.ru/item.asp?id=50101709
U2 - 10.3390/diagnostics13010140
DO - 10.3390/diagnostics13010140
M3 - Article
C2 - 36611432
VL - 13
JO - Diagnostics
JF - Diagnostics
SN - 2075-4418
IS - 1
M1 - 140
ER -
ID: 42508673