Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Cellular imaging by green luminescence of Tb(III)-doped aminomodified silica nanoparticles. / Fedorenko, Svetlana V.; Mustafina, Asiya R.; Mukhametshina, Alsu R. и др.
в: Materials Science and Engineering C, Том 76, 01.07.2017, стр. 551-558.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Cellular imaging by green luminescence of Tb(III)-doped aminomodified silica nanoparticles
AU - Fedorenko, Svetlana V.
AU - Mustafina, Asiya R.
AU - Mukhametshina, Alsu R.
AU - Jilkin, Michail E.
AU - Mukhametzyanov, Timur A.
AU - Solovieva, Anastasiya O.
AU - Pozmogova, Tatiana N.
AU - Shestopalova, Lidiya V.
AU - Shestopalov, Michael A.
AU - Kholin, Kirill V.
AU - Osin, Yury N.
AU - Sinyashin, Oleg G.
N1 - Publisher Copyright: © 2017 Elsevier B.V.
PY - 2017/7/1
Y1 - 2017/7/1
N2 - The work introduces Tb(III)-centered luminescence of amino-modified silica nanoparticles doped with Tb(III) complexes for cellular imaging. For these purposes water-in-oil procedure was optimized for synthesis of 20 and 35 nm luminescent nanoparticles with amino-groups embedded on the surface. The obtained results indicate an impact of the nanoparticle size in decoration, aggregation behavior and luminescent properties of the nanoparticles in protein-based buffer solutions. Formation of a protein-based corona on the nanoparticles surface was revealed through the effect of the nanoparticles on helical superstructure of BSA. This effect is evident from CD spectral data, while no any size impact on the adsorption of BSA onto aminomodified silica surface was observed. Cellular uptake of the nanoparticles studied by confocal and TEM microscopy methods indicates greater cellular uptake for the smaller nanoparticles. Cytotoxicity of the nanoparticles was found to agree well with their cellular uptake behavior, which in turn was found to be greater for the smaller nanoparticles.
AB - The work introduces Tb(III)-centered luminescence of amino-modified silica nanoparticles doped with Tb(III) complexes for cellular imaging. For these purposes water-in-oil procedure was optimized for synthesis of 20 and 35 nm luminescent nanoparticles with amino-groups embedded on the surface. The obtained results indicate an impact of the nanoparticle size in decoration, aggregation behavior and luminescent properties of the nanoparticles in protein-based buffer solutions. Formation of a protein-based corona on the nanoparticles surface was revealed through the effect of the nanoparticles on helical superstructure of BSA. This effect is evident from CD spectral data, while no any size impact on the adsorption of BSA onto aminomodified silica surface was observed. Cellular uptake of the nanoparticles studied by confocal and TEM microscopy methods indicates greater cellular uptake for the smaller nanoparticles. Cytotoxicity of the nanoparticles was found to agree well with their cellular uptake behavior, which in turn was found to be greater for the smaller nanoparticles.
KW - Cellular uptake
KW - Cytotoxicity
KW - Lanthanide complex
KW - Luminescence
KW - Protein-corona
KW - Silica nanoparticles
KW - Size-effect
KW - CELLS
KW - SURFACE FUNCTIONALIZATION
KW - SIZE
KW - NANOMATERIALS
KW - IONS
KW - COLLOIDAL STABILITY
KW - INTERNALIZATION
KW - ENERGY-TRANSFER
KW - CYTOTOXICITY
KW - METAL-COMPLEXES
KW - Water
KW - Silicon Dioxide
KW - Metal Nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=85015770728&partnerID=8YFLogxK
U2 - 10.1016/j.msec.2017.03.106
DO - 10.1016/j.msec.2017.03.106
M3 - Article
C2 - 28482563
AN - SCOPUS:85015770728
VL - 76
SP - 551
EP - 558
JO - Materials Science and Engineering C
JF - Materials Science and Engineering C
SN - 0928-4931
ER -
ID: 9029412