TY - JOUR

T1 - Biochemical response of two earthworm taxa exposed to freezing

AU - Shekhovtsov, Sergei V

AU - Zelentsova, Ekaterina A

AU - Bulakhova, Nina A

AU - Meshcheryakova, Ekaterina N

AU - Shishikina, Ksenia I

AU - Tsentalovich, Yuri P

AU - Berman, Daniil I

N1 - Funding: This study was supported by the State Budget Projects no. 1021060307698-5 (Faunogenesis and adaptive strategies of poikilothermic animals in extreme environments of the North) and no. FWNR-2022-0022. © 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.

PY - 2023/8

Y1 - 2023/8

N2 - Several earthworm species are known to be able to withstand freezing. At the biochemical level, this ability is based on cryoprotectant accumulation as well as several other mechanisms. In this study, we used 1H NMR to investigate metabolomic changes in two freeze-tolerant earthworm taxa, Dendrobaena octaedra and one of the genetic lineages of Eisenia sp. aff. nordenskioldi f. pallida. A total of 45 metabolites were quantified. High concentrations of glucose were present in frozen tissues of both taxa. No other putative cryoprotectants were found. We detected high levels of glycolysis end products and succinate in frozen animals, indicating the activation of glycolysis. Concentrations of many other substances also significantly increased. On the whole, metabolic change in response to freezing was much more pronounced in the specimens of Eisenia sp. aff. nordenskioldi f. pallida, including signs of nucleotide degradation.

AB - Several earthworm species are known to be able to withstand freezing. At the biochemical level, this ability is based on cryoprotectant accumulation as well as several other mechanisms. In this study, we used 1H NMR to investigate metabolomic changes in two freeze-tolerant earthworm taxa, Dendrobaena octaedra and one of the genetic lineages of Eisenia sp. aff. nordenskioldi f. pallida. A total of 45 metabolites were quantified. High concentrations of glucose were present in frozen tissues of both taxa. No other putative cryoprotectants were found. We detected high levels of glycolysis end products and succinate in frozen animals, indicating the activation of glycolysis. Concentrations of many other substances also significantly increased. On the whole, metabolic change in response to freezing was much more pronounced in the specimens of Eisenia sp. aff. nordenskioldi f. pallida, including signs of nucleotide degradation.

KW - Cryoprotectants

KW - Earthworms

KW - Freeze tolerance

KW - Glucose

KW - Metabolomics

KW - Oligochaeta/physiology

KW - Animals

KW - Glucose/metabolism

KW - Freezing

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85160861135&origin=inward&txGid=529faa06a0a43515f2902ee9bb006e91

UR - https://www.mendeley.com/catalogue/190f10a1-f2e6-32b9-bcab-dbf12a9954a5/

U2 - 10.1007/s00360-023-01500-w

DO - 10.1007/s00360-023-01500-w

M3 - Article

C2 - 37266592

VL - 193

SP - 391

EP - 400

JO - Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology

JF - Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology

SN - 0174-1578

IS - 4

ER -