Research output: Contribution to journal › Article › peer-review
Biochemical response of two earthworm taxa exposed to freezing. / Shekhovtsov, Sergei V; Zelentsova, Ekaterina A; Bulakhova, Nina A et al.
In: Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology, Vol. 193, No. 4, 08.2023, p. 391-400.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Biochemical response of two earthworm taxa exposed to freezing
AU - Shekhovtsov, Sergei V
AU - Zelentsova, Ekaterina A
AU - Bulakhova, Nina A
AU - Meshcheryakova, Ekaterina N
AU - Shishikina, Ksenia I
AU - Tsentalovich, Yuri P
AU - Berman, Daniil I
N1 - Funding: This study was supported by the State Budget Projects no. 1021060307698-5 (Faunogenesis and adaptive strategies of poikilothermic animals in extreme environments of the North) and no. FWNR-2022-0022. © 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, part of Springer Nature.
PY - 2023/8
Y1 - 2023/8
N2 - Several earthworm species are known to be able to withstand freezing. At the biochemical level, this ability is based on cryoprotectant accumulation as well as several other mechanisms. In this study, we used 1H NMR to investigate metabolomic changes in two freeze-tolerant earthworm taxa, Dendrobaena octaedra and one of the genetic lineages of Eisenia sp. aff. nordenskioldi f. pallida. A total of 45 metabolites were quantified. High concentrations of glucose were present in frozen tissues of both taxa. No other putative cryoprotectants were found. We detected high levels of glycolysis end products and succinate in frozen animals, indicating the activation of glycolysis. Concentrations of many other substances also significantly increased. On the whole, metabolic change in response to freezing was much more pronounced in the specimens of Eisenia sp. aff. nordenskioldi f. pallida, including signs of nucleotide degradation.
AB - Several earthworm species are known to be able to withstand freezing. At the biochemical level, this ability is based on cryoprotectant accumulation as well as several other mechanisms. In this study, we used 1H NMR to investigate metabolomic changes in two freeze-tolerant earthworm taxa, Dendrobaena octaedra and one of the genetic lineages of Eisenia sp. aff. nordenskioldi f. pallida. A total of 45 metabolites were quantified. High concentrations of glucose were present in frozen tissues of both taxa. No other putative cryoprotectants were found. We detected high levels of glycolysis end products and succinate in frozen animals, indicating the activation of glycolysis. Concentrations of many other substances also significantly increased. On the whole, metabolic change in response to freezing was much more pronounced in the specimens of Eisenia sp. aff. nordenskioldi f. pallida, including signs of nucleotide degradation.
KW - Cryoprotectants
KW - Earthworms
KW - Freeze tolerance
KW - Glucose
KW - Metabolomics
KW - Oligochaeta/physiology
KW - Animals
KW - Glucose/metabolism
KW - Freezing
UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85160861135&origin=inward&txGid=529faa06a0a43515f2902ee9bb006e91
UR - https://www.mendeley.com/catalogue/190f10a1-f2e6-32b9-bcab-dbf12a9954a5/
U2 - 10.1007/s00360-023-01500-w
DO - 10.1007/s00360-023-01500-w
M3 - Article
C2 - 37266592
VL - 193
SP - 391
EP - 400
JO - Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology
JF - Journal of comparative physiology. B, Biochemical, systemic, and environmental physiology
SN - 0174-1578
IS - 4
ER -
ID: 50660550