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B cell-specific protein FCRLA is expressed by plasmacytoid dendritic cells in humans. / Reshetnikova, Evdokiya; Guselnikov, Sergey; Volkova, Olga и др.

в: Cytometry Part B - Clinical Cytometry, Том 94, № 4, 01.07.2018, стр. 683-687.

Результаты исследований: Научные публикации в периодических изданияхстатьяРецензирование

Harvard

Reshetnikova, E, Guselnikov, S, Volkova, O, Baranov, K, Taranin, A & Mechetina, L 2018, 'B cell-specific protein FCRLA is expressed by plasmacytoid dendritic cells in humans', Cytometry Part B - Clinical Cytometry, Том. 94, № 4, стр. 683-687. https://doi.org/10.1002/cyto.b.21611

APA

Reshetnikova, E., Guselnikov, S., Volkova, O., Baranov, K., Taranin, A., & Mechetina, L. (2018). B cell-specific protein FCRLA is expressed by plasmacytoid dendritic cells in humans. Cytometry Part B - Clinical Cytometry, 94(4), 683-687. https://doi.org/10.1002/cyto.b.21611

Vancouver

Reshetnikova E, Guselnikov S, Volkova O, Baranov K, Taranin A, Mechetina L. B cell-specific protein FCRLA is expressed by plasmacytoid dendritic cells in humans. Cytometry Part B - Clinical Cytometry. 2018 июль 1;94(4):683-687. doi: 10.1002/cyto.b.21611

Author

Reshetnikova, Evdokiya ; Guselnikov, Sergey ; Volkova, Olga и др. / B cell-specific protein FCRLA is expressed by plasmacytoid dendritic cells in humans. в: Cytometry Part B - Clinical Cytometry. 2018 ; Том 94, № 4. стр. 683-687.

BibTeX

@article{853acad40e2b4740916aa24d8f3052d7,
title = "B cell-specific protein FCRLA is expressed by plasmacytoid dendritic cells in humans",
abstract = "Background: Fc receptor-like A (FCRLA) is a unique member of a family of Fc receptor like-molecules that lacks a transmembrane region and is an ER-resident protein. In mice and humans, FCRLA has been known as a B cell specific protein. We report here that, in humans, FCRLA is also expressed in a subpopulation of plasmacytoid dendritic cells (pDCs). Methods: Human peripheral blood mononuclear cells (PBMC), splenocytes, and tonsillar cells were stained for lineage markers followed by fixation/saponin permeabilization and intracellular staining for FCRLA, and then analyzed by flow cytometry with CD123 and CD303 used as pDC markers. Results: We conducted an extensive flow cytometric analysis of a rare population of CD19-FCRLA+ cells found for the first time in human lymphoid tissues that we assigned to pDCs as they were lin-/CD123+/CD303+. FCRLA expression in human pDCs was further confirmed by the RT-PCR analysis of cDNA of pDCs isolated from the peripheral blood of a healthy donor. FCRLA-positive pDCs expressed a lower level of HLA-DR than their FCRLA-negative counterparts. Conclusions: FCRLA has long been viewed as a B cell specific protein, and this is the first time its expression has also been shown in human pDCs.",
keywords = "FCRLA, Flow cytometry, Human PBMC, Plasmacytoid dendritic cells, plasmacytoid dendritic cells, SUBSETS, IGG, flow cytometry, PERIPHERAL-BLOOD, human PBMC, ANTIGEN, Humans, Flow Cytometry, Receptors, Immunologic/biosynthesis, Dendritic Cells/immunology",
author = "Evdokiya Reshetnikova and Sergey Guselnikov and Olga Volkova and Konstantin Baranov and Alexander Taranin and Ludmila Mechetina",
note = "Publisher Copyright: {\textcopyright} 2017 International Clinical Cytometry Society",
year = "2018",
month = jul,
day = "1",
doi = "10.1002/cyto.b.21611",
language = "English",
volume = "94",
pages = "683--687",
journal = "Cytometry Part B - Clinical Cytometry",
issn = "1552-4949",
publisher = "Wiley-Liss Inc.",
number = "4",

}

RIS

TY - JOUR

T1 - B cell-specific protein FCRLA is expressed by plasmacytoid dendritic cells in humans

AU - Reshetnikova, Evdokiya

AU - Guselnikov, Sergey

AU - Volkova, Olga

AU - Baranov, Konstantin

AU - Taranin, Alexander

AU - Mechetina, Ludmila

N1 - Publisher Copyright: © 2017 International Clinical Cytometry Society

PY - 2018/7/1

Y1 - 2018/7/1

N2 - Background: Fc receptor-like A (FCRLA) is a unique member of a family of Fc receptor like-molecules that lacks a transmembrane region and is an ER-resident protein. In mice and humans, FCRLA has been known as a B cell specific protein. We report here that, in humans, FCRLA is also expressed in a subpopulation of plasmacytoid dendritic cells (pDCs). Methods: Human peripheral blood mononuclear cells (PBMC), splenocytes, and tonsillar cells were stained for lineage markers followed by fixation/saponin permeabilization and intracellular staining for FCRLA, and then analyzed by flow cytometry with CD123 and CD303 used as pDC markers. Results: We conducted an extensive flow cytometric analysis of a rare population of CD19-FCRLA+ cells found for the first time in human lymphoid tissues that we assigned to pDCs as they were lin-/CD123+/CD303+. FCRLA expression in human pDCs was further confirmed by the RT-PCR analysis of cDNA of pDCs isolated from the peripheral blood of a healthy donor. FCRLA-positive pDCs expressed a lower level of HLA-DR than their FCRLA-negative counterparts. Conclusions: FCRLA has long been viewed as a B cell specific protein, and this is the first time its expression has also been shown in human pDCs.

AB - Background: Fc receptor-like A (FCRLA) is a unique member of a family of Fc receptor like-molecules that lacks a transmembrane region and is an ER-resident protein. In mice and humans, FCRLA has been known as a B cell specific protein. We report here that, in humans, FCRLA is also expressed in a subpopulation of plasmacytoid dendritic cells (pDCs). Methods: Human peripheral blood mononuclear cells (PBMC), splenocytes, and tonsillar cells were stained for lineage markers followed by fixation/saponin permeabilization and intracellular staining for FCRLA, and then analyzed by flow cytometry with CD123 and CD303 used as pDC markers. Results: We conducted an extensive flow cytometric analysis of a rare population of CD19-FCRLA+ cells found for the first time in human lymphoid tissues that we assigned to pDCs as they were lin-/CD123+/CD303+. FCRLA expression in human pDCs was further confirmed by the RT-PCR analysis of cDNA of pDCs isolated from the peripheral blood of a healthy donor. FCRLA-positive pDCs expressed a lower level of HLA-DR than their FCRLA-negative counterparts. Conclusions: FCRLA has long been viewed as a B cell specific protein, and this is the first time its expression has also been shown in human pDCs.

KW - FCRLA

KW - Flow cytometry

KW - Human PBMC

KW - Plasmacytoid dendritic cells

KW - plasmacytoid dendritic cells

KW - SUBSETS

KW - IGG

KW - flow cytometry

KW - PERIPHERAL-BLOOD

KW - human PBMC

KW - ANTIGEN

KW - Humans

KW - Flow Cytometry

KW - Receptors, Immunologic/biosynthesis

KW - Dendritic Cells/immunology

UR - http://www.scopus.com/inward/record.url?scp=85040737959&partnerID=8YFLogxK

U2 - 10.1002/cyto.b.21611

DO - 10.1002/cyto.b.21611

M3 - Article

C2 - 29236355

AN - SCOPUS:85040737959

VL - 94

SP - 683

EP - 687

JO - Cytometry Part B - Clinical Cytometry

JF - Cytometry Part B - Clinical Cytometry

SN - 1552-4949

IS - 4

ER -

ID: 12100033