Research output: Contribution to journal › Article › peer-review
B cell-specific protein FCRLA is expressed by plasmacytoid dendritic cells in humans. / Reshetnikova, Evdokiya; Guselnikov, Sergey; Volkova, Olga et al.
In: Cytometry Part B - Clinical Cytometry, Vol. 94, No. 4, 01.07.2018, p. 683-687.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - B cell-specific protein FCRLA is expressed by plasmacytoid dendritic cells in humans
AU - Reshetnikova, Evdokiya
AU - Guselnikov, Sergey
AU - Volkova, Olga
AU - Baranov, Konstantin
AU - Taranin, Alexander
AU - Mechetina, Ludmila
N1 - Publisher Copyright: © 2017 International Clinical Cytometry Society
PY - 2018/7/1
Y1 - 2018/7/1
N2 - Background: Fc receptor-like A (FCRLA) is a unique member of a family of Fc receptor like-molecules that lacks a transmembrane region and is an ER-resident protein. In mice and humans, FCRLA has been known as a B cell specific protein. We report here that, in humans, FCRLA is also expressed in a subpopulation of plasmacytoid dendritic cells (pDCs). Methods: Human peripheral blood mononuclear cells (PBMC), splenocytes, and tonsillar cells were stained for lineage markers followed by fixation/saponin permeabilization and intracellular staining for FCRLA, and then analyzed by flow cytometry with CD123 and CD303 used as pDC markers. Results: We conducted an extensive flow cytometric analysis of a rare population of CD19-FCRLA+ cells found for the first time in human lymphoid tissues that we assigned to pDCs as they were lin-/CD123+/CD303+. FCRLA expression in human pDCs was further confirmed by the RT-PCR analysis of cDNA of pDCs isolated from the peripheral blood of a healthy donor. FCRLA-positive pDCs expressed a lower level of HLA-DR than their FCRLA-negative counterparts. Conclusions: FCRLA has long been viewed as a B cell specific protein, and this is the first time its expression has also been shown in human pDCs.
AB - Background: Fc receptor-like A (FCRLA) is a unique member of a family of Fc receptor like-molecules that lacks a transmembrane region and is an ER-resident protein. In mice and humans, FCRLA has been known as a B cell specific protein. We report here that, in humans, FCRLA is also expressed in a subpopulation of plasmacytoid dendritic cells (pDCs). Methods: Human peripheral blood mononuclear cells (PBMC), splenocytes, and tonsillar cells were stained for lineage markers followed by fixation/saponin permeabilization and intracellular staining for FCRLA, and then analyzed by flow cytometry with CD123 and CD303 used as pDC markers. Results: We conducted an extensive flow cytometric analysis of a rare population of CD19-FCRLA+ cells found for the first time in human lymphoid tissues that we assigned to pDCs as they were lin-/CD123+/CD303+. FCRLA expression in human pDCs was further confirmed by the RT-PCR analysis of cDNA of pDCs isolated from the peripheral blood of a healthy donor. FCRLA-positive pDCs expressed a lower level of HLA-DR than their FCRLA-negative counterparts. Conclusions: FCRLA has long been viewed as a B cell specific protein, and this is the first time its expression has also been shown in human pDCs.
KW - FCRLA
KW - Flow cytometry
KW - Human PBMC
KW - Plasmacytoid dendritic cells
KW - plasmacytoid dendritic cells
KW - SUBSETS
KW - IGG
KW - flow cytometry
KW - PERIPHERAL-BLOOD
KW - human PBMC
KW - ANTIGEN
KW - Humans
KW - Flow Cytometry
KW - Receptors, Immunologic/biosynthesis
KW - Dendritic Cells/immunology
UR - http://www.scopus.com/inward/record.url?scp=85040737959&partnerID=8YFLogxK
U2 - 10.1002/cyto.b.21611
DO - 10.1002/cyto.b.21611
M3 - Article
C2 - 29236355
AN - SCOPUS:85040737959
VL - 94
SP - 683
EP - 687
JO - Cytometry Part B - Clinical Cytometry
JF - Cytometry Part B - Clinical Cytometry
SN - 1552-4949
IS - 4
ER -
ID: 12100033