TY - JOUR

T1 - Assessment of the Cytokine-Producing Resource of Tumor Biopsy Samples from Patients with Invasive Carcinoma of No Special Type and with Non-Malignant Breast Diseases

AU - Autenshlyus, A. I.

AU - Studenikina, A. A.

AU - Bernado, A. V.

AU - Mikhailova, E. S.

AU - Proskura, A. V.

AU - Sidorov, S. V.

AU - Varaksin, N. A.

AU - Lyakhovich, V. V.

PY - 2020/1/1

Y1 - 2020/1/1

N2 - In most cases, breast cancer is a malignant neoplasm associated with infiltration of a tumor with the cells that form its microenvironment and produce various cytokines. The aim of the study was to evaluate the cytokine-producing function of tumor cells and their microenvironment in biopsy samples of patients with invasive breast carcinoma of no special type (IBC-NST; group I) and in patients with benign breast diseases (BBD, group II). Patients of group II were further divided into group IIa, which included patients only with fibroadenoma, and group IIb, which included patients with leaf-shaped fibroadenoma, fibroadenomatosis, fibrocystic mastopathy, intraductal papillomatosis and sclerosing adenosis and fibrocystic mastopathy microcalcifications. Concentrations of IL-2, IL-6, IL-8, IL-10, IL-17, IL-18, IL-1β, IL-1Ra, TNF-α, IFN-γ, G-CSF, GM-CSF, VEGF, and MCP-1 were measured in supernatants of breast biopsy samples. The cytokine-producing activity of tumor biopsy samples and its microenvironment was assessed by the index of the polyclonal activator influence (IPAI) on cytokine production. Comparison of groups I and II, revealed high IPAI values on production of IL-17, IL-18 and TNF-α in BBD patients (group II). Higher IPAI values on production of IL-18, TNF-α, and IL-1β and the ratio of IL1β/IL1Ra were observed in BDD patients with fibroadenoma as compared to IBC-NST patients. No statistically significant differences in the IPAI values were found between groups I and IIb. This suggests the existence of changes in the mammary gland in patients of group IIb similar to those in breast carcinoma (IBC-NST) patients. Comparison of groups IIa and IIb revealed higher IPAI values in group IIa (fibroadenoma) patients on production of IL-1β, as well as the ratio of IL1β/IL1Ra. The results of this study have shown some features of the cytokine-producing resource of breast biopsy samples from patents with IBC-NST and BDD.

AB - In most cases, breast cancer is a malignant neoplasm associated with infiltration of a tumor with the cells that form its microenvironment and produce various cytokines. The aim of the study was to evaluate the cytokine-producing function of tumor cells and their microenvironment in biopsy samples of patients with invasive breast carcinoma of no special type (IBC-NST; group I) and in patients with benign breast diseases (BBD, group II). Patients of group II were further divided into group IIa, which included patients only with fibroadenoma, and group IIb, which included patients with leaf-shaped fibroadenoma, fibroadenomatosis, fibrocystic mastopathy, intraductal papillomatosis and sclerosing adenosis and fibrocystic mastopathy microcalcifications. Concentrations of IL-2, IL-6, IL-8, IL-10, IL-17, IL-18, IL-1β, IL-1Ra, TNF-α, IFN-γ, G-CSF, GM-CSF, VEGF, and MCP-1 were measured in supernatants of breast biopsy samples. The cytokine-producing activity of tumor biopsy samples and its microenvironment was assessed by the index of the polyclonal activator influence (IPAI) on cytokine production. Comparison of groups I and II, revealed high IPAI values on production of IL-17, IL-18 and TNF-α in BBD patients (group II). Higher IPAI values on production of IL-18, TNF-α, and IL-1β and the ratio of IL1β/IL1Ra were observed in BDD patients with fibroadenoma as compared to IBC-NST patients. No statistically significant differences in the IPAI values were found between groups I and IIb. This suggests the existence of changes in the mammary gland in patients of group IIb similar to those in breast carcinoma (IBC-NST) patients. Comparison of groups IIa and IIb revealed higher IPAI values in group IIa (fibroadenoma) patients on production of IL-1β, as well as the ratio of IL1β/IL1Ra. The results of this study have shown some features of the cytokine-producing resource of breast biopsy samples from patents with IBC-NST and BDD.

KW - breast diseases

KW - cytokines

KW - tumor microenvironment

KW - CELLS

KW - WOMEN

KW - CANCER RISK

KW - SUBTYPES

KW - EXPRESSION

UR - http://www.scopus.com/inward/record.url?scp=85081280037&partnerID=8YFLogxK

U2 - 10.1134/S1990750820010047

DO - 10.1134/S1990750820010047

M3 - Article

AN - SCOPUS:85081280037

VL - 14

SP - 38

EP - 43

JO - Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry

JF - Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry

SN - 1990-7508

IS - 1

ER -