Assessment of the Cytokine-Producing Resource of Tumor Biopsy Samples from Patients with Invasive Carcinoma of No Special Type and with Non-Malignant Breast Diseases. / Autenshlyus, A. I.; Studenikina, A. A.; Bernado, A. V. et al.
In: Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry, Vol. 14, No. 1, 01.01.2020, p. 38-43.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Assessment of the Cytokine-Producing Resource of Tumor Biopsy Samples from Patients with Invasive Carcinoma of No Special Type and with Non-Malignant Breast Diseases
AU - Autenshlyus, A. I.
AU - Studenikina, A. A.
AU - Bernado, A. V.
AU - Mikhailova, E. S.
AU - Proskura, A. V.
AU - Sidorov, S. V.
AU - Varaksin, N. A.
AU - Lyakhovich, V. V.
PY - 2020/1/1
Y1 - 2020/1/1
N2 - In most cases, breast cancer is a malignant neoplasm associated with infiltration of a tumor with the cells that form its microenvironment and produce various cytokines. The aim of the study was to evaluate the cytokine-producing function of tumor cells and their microenvironment in biopsy samples of patients with invasive breast carcinoma of no special type (IBC-NST; group I) and in patients with benign breast diseases (BBD, group II). Patients of group II were further divided into group IIa, which included patients only with fibroadenoma, and group IIb, which included patients with leaf-shaped fibroadenoma, fibroadenomatosis, fibrocystic mastopathy, intraductal papillomatosis and sclerosing adenosis and fibrocystic mastopathy microcalcifications. Concentrations of IL-2, IL-6, IL-8, IL-10, IL-17, IL-18, IL-1β, IL-1Ra, TNF-α, IFN-γ, G-CSF, GM-CSF, VEGF, and MCP-1 were measured in supernatants of breast biopsy samples. The cytokine-producing activity of tumor biopsy samples and its microenvironment was assessed by the index of the polyclonal activator influence (IPAI) on cytokine production. Comparison of groups I and II, revealed high IPAI values on production of IL-17, IL-18 and TNF-α in BBD patients (group II). Higher IPAI values on production of IL-18, TNF-α, and IL-1β and the ratio of IL1β/IL1Ra were observed in BDD patients with fibroadenoma as compared to IBC-NST patients. No statistically significant differences in the IPAI values were found between groups I and IIb. This suggests the existence of changes in the mammary gland in patients of group IIb similar to those in breast carcinoma (IBC-NST) patients. Comparison of groups IIa and IIb revealed higher IPAI values in group IIa (fibroadenoma) patients on production of IL-1β, as well as the ratio of IL1β/IL1Ra. The results of this study have shown some features of the cytokine-producing resource of breast biopsy samples from patents with IBC-NST and BDD.
AB - In most cases, breast cancer is a malignant neoplasm associated with infiltration of a tumor with the cells that form its microenvironment and produce various cytokines. The aim of the study was to evaluate the cytokine-producing function of tumor cells and their microenvironment in biopsy samples of patients with invasive breast carcinoma of no special type (IBC-NST; group I) and in patients with benign breast diseases (BBD, group II). Patients of group II were further divided into group IIa, which included patients only with fibroadenoma, and group IIb, which included patients with leaf-shaped fibroadenoma, fibroadenomatosis, fibrocystic mastopathy, intraductal papillomatosis and sclerosing adenosis and fibrocystic mastopathy microcalcifications. Concentrations of IL-2, IL-6, IL-8, IL-10, IL-17, IL-18, IL-1β, IL-1Ra, TNF-α, IFN-γ, G-CSF, GM-CSF, VEGF, and MCP-1 were measured in supernatants of breast biopsy samples. The cytokine-producing activity of tumor biopsy samples and its microenvironment was assessed by the index of the polyclonal activator influence (IPAI) on cytokine production. Comparison of groups I and II, revealed high IPAI values on production of IL-17, IL-18 and TNF-α in BBD patients (group II). Higher IPAI values on production of IL-18, TNF-α, and IL-1β and the ratio of IL1β/IL1Ra were observed in BDD patients with fibroadenoma as compared to IBC-NST patients. No statistically significant differences in the IPAI values were found between groups I and IIb. This suggests the existence of changes in the mammary gland in patients of group IIb similar to those in breast carcinoma (IBC-NST) patients. Comparison of groups IIa and IIb revealed higher IPAI values in group IIa (fibroadenoma) patients on production of IL-1β, as well as the ratio of IL1β/IL1Ra. The results of this study have shown some features of the cytokine-producing resource of breast biopsy samples from patents with IBC-NST and BDD.
KW - breast diseases
KW - cytokines
KW - tumor microenvironment
KW - CELLS
KW - WOMEN
KW - CANCER RISK
KW - SUBTYPES
KW - EXPRESSION
UR - http://www.scopus.com/inward/record.url?scp=85081280037&partnerID=8YFLogxK
U2 - 10.1134/S1990750820010047
DO - 10.1134/S1990750820010047
M3 - Article
AN - SCOPUS:85081280037
VL - 14
SP - 38
EP - 43
JO - Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry
JF - Biochemistry (Moscow) Supplement Series B: Biomedical Chemistry
SN - 1990-7508
IS - 1
ER -
ID: 23801543