TY - JOUR

T1 - Ant13 Encodes Regulatory Factor WD40 Controlling Anthocyanin and Proanthocyanidin Synthesis in Barley (Hordeum vulgare L.)

AU - Shoeva, Olesya Yu

AU - Mukhanova, Marina A

AU - Zakhrabekova, Shakhira

AU - Hansson, Mats

N1 - Funding: The study was partially supported by ICG (project FWNR2022-0017), the Kurchatov Center for Genomic Research at the Institute of Cytology and Genetics (project no. 075-15-2019-1662), the Swedish Institute Visby Programme (25896/ 2018), the Swedish Research Council (VR 2018-05117), the Swedish Research Council for Environment, Agricultural Sciences, and Spatial Planning (FORMAS 2018-01026), the Erik Philip-Sörensen Foundation, and the Royal Physiographic Society in Lund.

PY - 2023/5/10

Y1 - 2023/5/10

N2 - Flavonoid compounds like anthocyanins and proanthocyanidins are important plant secondary metabolites having wide biological activities for humans. In this study, the molecular function of the Ant13 locus, which is one of the key loci governing flavonoid synthesis in barley, was determined. It was found that Ant13 encodes a WD40-type regulatory protein, which is required for transcriptional activation of a set of structural genes encoding enzymes of flavonoid biosynthesis at the leaf sheath base (colored by anthocyanins) and in grains (which accumulate proanthocyanidins). Besides its role in flavonoid biosynthesis, pleiotropic effects of this gene in plant growth were revealed. The mutants deficient in the Ant13 locus showed similar germination rates but a decreased rate of root and shoot growth and yield-related parameters in comparison to the parental cultivars. This is the seventh Ant locus (among 30) for which molecular functions in flavonoid biosynthesis regulation have been determined.

AB - Flavonoid compounds like anthocyanins and proanthocyanidins are important plant secondary metabolites having wide biological activities for humans. In this study, the molecular function of the Ant13 locus, which is one of the key loci governing flavonoid synthesis in barley, was determined. It was found that Ant13 encodes a WD40-type regulatory protein, which is required for transcriptional activation of a set of structural genes encoding enzymes of flavonoid biosynthesis at the leaf sheath base (colored by anthocyanins) and in grains (which accumulate proanthocyanidins). Besides its role in flavonoid biosynthesis, pleiotropic effects of this gene in plant growth were revealed. The mutants deficient in the Ant13 locus showed similar germination rates but a decreased rate of root and shoot growth and yield-related parameters in comparison to the parental cultivars. This is the seventh Ant locus (among 30) for which molecular functions in flavonoid biosynthesis regulation have been determined.

KW - anthocyanin-less

KW - condensed tannin

KW - flavonoid

KW - haze

KW - malt

KW - regulatory gene

KW - Proanthocyanidins/metabolism

KW - Humans

KW - Transcription Factors/genetics

KW - Hordeum/genetics

KW - Plant Proteins/genetics

KW - Flavonoids/metabolism

KW - Gene Expression Regulation, Plant

KW - Anthocyanins/metabolism

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85156274033&origin=inward&txGid=1c47623a5b6b2e831a699a26d34ef0aa

UR - https://www.mendeley.com/catalogue/1efb4022-d8ce-3319-b2bc-4830f44a7202/

U2 - 10.1021/acs.jafc.2c09051

DO - 10.1021/acs.jafc.2c09051

M3 - Article

C2 - 37104658

VL - 71

SP - 6967

EP - 6977

JO - Journal of agricultural and food chemistry

JF - Journal of agricultural and food chemistry

SN - 0021-8561

IS - 18

ER -