TY - JOUR

T1 - Anodic stripping voltammetry for direct determination of heavy metals in bovine seminal plasma using thick film modified graphite electrodes

AU - Skiba, Tatyana V.

AU - Gou, Hueyhuey

PY - 2019/6/1

Y1 - 2019/6/1

N2 - The thick-film modified graphite sensors (TMGE) as working electrodes are used for determination of heavy metals in bovine seminal plasma by anodic stripping voltammetry. The technique proposed is a very simple, express and no any sample preparation such as acid deproteinization or autoclave digestion is necessary. The sample of seminal plasma is just diluted with electrolyte solution in the analysis process. The aliquot of the sample is injected in the electrochemical cell and the analytical signal is measured. The analytical signal dependences on the accumulation potential, accumulation time and sweep rate have been studied. The standard addition method is used for calculating of heavy metal concentrations in the samples. The accuracy of the developed technique is proved by comparison with the results obtained by the independent method of ICP-AES (atomic emission spectrometry with inductively coupled plasma). The detection limits (3SD blank ) are found to be 3.6 μg·L −1 for Pb and 40 μg·L −1 for Cu in bovine seminal plasma (taking into account sample dilution). The relative standard deviation (RSD) obtained by the proposed technique do not exceed 13%.

AB - The thick-film modified graphite sensors (TMGE) as working electrodes are used for determination of heavy metals in bovine seminal plasma by anodic stripping voltammetry. The technique proposed is a very simple, express and no any sample preparation such as acid deproteinization or autoclave digestion is necessary. The sample of seminal plasma is just diluted with electrolyte solution in the analysis process. The aliquot of the sample is injected in the electrochemical cell and the analytical signal is measured. The analytical signal dependences on the accumulation potential, accumulation time and sweep rate have been studied. The standard addition method is used for calculating of heavy metal concentrations in the samples. The accuracy of the developed technique is proved by comparison with the results obtained by the independent method of ICP-AES (atomic emission spectrometry with inductively coupled plasma). The detection limits (3SD blank ) are found to be 3.6 μg·L −1 for Pb and 40 μg·L −1 for Cu in bovine seminal plasma (taking into account sample dilution). The relative standard deviation (RSD) obtained by the proposed technique do not exceed 13%.

KW - Film graphite electrode

KW - Heavy metals

KW - Seminal plasma

KW - Stripping voltammetry

KW - QUALITY

KW - COPPER

KW - SEMEN PARAMETERS

KW - ZINC

KW - MAGNESIUM

KW - TRACE-ELEMENTS

KW - CADMIUM

KW - CALCIUM

KW - LEAD

KW - BLOOD

UR - http://www.scopus.com/inward/record.url?scp=85063881516&partnerID=8YFLogxK

U2 - 10.1016/j.microc.2019.03.091

DO - 10.1016/j.microc.2019.03.091

M3 - Article

AN - SCOPUS:85063881516

VL - 147

SP - 818

EP - 823

JO - Microchemical Journal

JF - Microchemical Journal

SN - 0026-265X

ER -