Research output: Contribution to journal › Article › peer-review
Anodic stripping voltammetry for direct determination of heavy metals in bovine seminal plasma using thick film modified graphite electrodes. / Skiba, Tatyana V.; Gou, Hueyhuey.
In: Microchemical Journal, Vol. 147, 01.06.2019, p. 818-823.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Anodic stripping voltammetry for direct determination of heavy metals in bovine seminal plasma using thick film modified graphite electrodes
AU - Skiba, Tatyana V.
AU - Gou, Hueyhuey
PY - 2019/6/1
Y1 - 2019/6/1
N2 - The thick-film modified graphite sensors (TMGE) as working electrodes are used for determination of heavy metals in bovine seminal plasma by anodic stripping voltammetry. The technique proposed is a very simple, express and no any sample preparation such as acid deproteinization or autoclave digestion is necessary. The sample of seminal plasma is just diluted with electrolyte solution in the analysis process. The aliquot of the sample is injected in the electrochemical cell and the analytical signal is measured. The analytical signal dependences on the accumulation potential, accumulation time and sweep rate have been studied. The standard addition method is used for calculating of heavy metal concentrations in the samples. The accuracy of the developed technique is proved by comparison with the results obtained by the independent method of ICP-AES (atomic emission spectrometry with inductively coupled plasma). The detection limits (3SD blank ) are found to be 3.6 μg·L −1 for Pb and 40 μg·L −1 for Cu in bovine seminal plasma (taking into account sample dilution). The relative standard deviation (RSD) obtained by the proposed technique do not exceed 13%.
AB - The thick-film modified graphite sensors (TMGE) as working electrodes are used for determination of heavy metals in bovine seminal plasma by anodic stripping voltammetry. The technique proposed is a very simple, express and no any sample preparation such as acid deproteinization or autoclave digestion is necessary. The sample of seminal plasma is just diluted with electrolyte solution in the analysis process. The aliquot of the sample is injected in the electrochemical cell and the analytical signal is measured. The analytical signal dependences on the accumulation potential, accumulation time and sweep rate have been studied. The standard addition method is used for calculating of heavy metal concentrations in the samples. The accuracy of the developed technique is proved by comparison with the results obtained by the independent method of ICP-AES (atomic emission spectrometry with inductively coupled plasma). The detection limits (3SD blank ) are found to be 3.6 μg·L −1 for Pb and 40 μg·L −1 for Cu in bovine seminal plasma (taking into account sample dilution). The relative standard deviation (RSD) obtained by the proposed technique do not exceed 13%.
KW - Film graphite electrode
KW - Heavy metals
KW - Seminal plasma
KW - Stripping voltammetry
KW - QUALITY
KW - COPPER
KW - SEMEN PARAMETERS
KW - ZINC
KW - MAGNESIUM
KW - TRACE-ELEMENTS
KW - CADMIUM
KW - CALCIUM
KW - LEAD
KW - BLOOD
UR - http://www.scopus.com/inward/record.url?scp=85063881516&partnerID=8YFLogxK
U2 - 10.1016/j.microc.2019.03.091
DO - 10.1016/j.microc.2019.03.091
M3 - Article
AN - SCOPUS:85063881516
VL - 147
SP - 818
EP - 823
JO - Microchemical Journal
JF - Microchemical Journal
SN - 0026-265X
ER -
ID: 19357372