Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Analysis of in vitro activity of PSCA-specific CARs in the context of human NK cell line YT. / Matvienko, Daria A.; Kulemzin, Sergey V.; Smagina, Anna S. и др.
в: Cellular Therapy and Transplantation, Том 7, № 2, 01.01.2018, стр. 70-77.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Analysis of in vitro activity of PSCA-specific CARs in the context of human NK cell line YT
AU - Matvienko, Daria A.
AU - Kulemzin, Sergey V.
AU - Smagina, Anna S.
AU - Belovezhets, Tatyana N.
AU - Chikaev, Anton N.
AU - Volkova, Olga Y.
AU - Chikaev, Nikolay A.
AU - Koval, Olga A.
AU - Kuligina, Elena V.
AU - Taranin, Alexandr V.
AU - Gorchakov, Andrey A.
N1 - Publisher Copyright: © 2018, Universitatsklinikum Hamburg - Eppendorf. All rights reserved.
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Comprehensive structural optimization of chimeric antigen receptors (CARs) is the key to their successful performance both in vitro and in vivo. In order to compare various CAR designs in an unified format, we took advantage of a lentiviral platform, where all CAR modules can be easily shuffled and tested for functionality. This platform was used to delineate the effects of various spacer regions on the function of a PSCA-specific CAR in the context of a human NK cell line, YT. We show that three CAR designs (IgG1-, CD8a-, and spacerless) perform similarly in vitro regardless of the length of the spacer region.
AB - Comprehensive structural optimization of chimeric antigen receptors (CARs) is the key to their successful performance both in vitro and in vivo. In order to compare various CAR designs in an unified format, we took advantage of a lentiviral platform, where all CAR modules can be easily shuffled and tested for functionality. This platform was used to delineate the effects of various spacer regions on the function of a PSCA-specific CAR in the context of a human NK cell line, YT. We show that three CAR designs (IgG1-, CD8a-, and spacerless) perform similarly in vitro regardless of the length of the spacer region.
KW - Adoptive immunotherapy
KW - Chimeric antigen receptor (CAR)
KW - Prostate cancer
KW - Prostate stem cell antigen (PSCA)
UR - http://www.scopus.com/inward/record.url?scp=85055341900&partnerID=8YFLogxK
U2 - 10.18620/ctt-1866-8836-2018-7-2-70-77
DO - 10.18620/ctt-1866-8836-2018-7-2-70-77
M3 - Article
AN - SCOPUS:85055341900
VL - 7
SP - 70
EP - 77
JO - Cellular Therapy and Transplantation
JF - Cellular Therapy and Transplantation
SN - 1867-416X
IS - 2
ER -
ID: 17249720