Analysis of in vitro activity of PSCA-specific CARs in the context of human NK cell line YT

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Analysis of in vitro activity of PSCA-specific CARs in the context of human NK cell line YT. / Matvienko, Daria A.; Kulemzin, Sergey V.; Smagina, Anna S. et al.

In: Cellular Therapy and Transplantation, Vol. 7, No. 2, 01.01.2018, p. 70-77.

Research output: Contribution to journal › Article › peer-review

Harvard

Matvienko, DA, Kulemzin, SV, Smagina, AS, Belovezhets, TN, Chikaev, AN, Volkova, OY, Chikaev, NA, Koval, OA, Kuligina, EV, Taranin, AV & Gorchakov, AA 2018, 'Analysis of in vitro activity of PSCA-specific CARs in the context of human NK cell line YT', Cellular Therapy and Transplantation, vol. 7, no. 2, pp. 70-77. https://doi.org/10.18620/ctt-1866-8836-2018-7-2-70-77

APA

Matvienko, D. A., Kulemzin, S. V., Smagina, A. S., Belovezhets, T. N., Chikaev, A. N., Volkova, O. Y., Chikaev, N. A., Koval, O. A., Kuligina, E. V., Taranin, A. V., & Gorchakov, A. A. (2018). Analysis of in vitro activity of PSCA-specific CARs in the context of human NK cell line YT. Cellular Therapy and Transplantation, 7(2), 70-77. https://doi.org/10.18620/ctt-1866-8836-2018-7-2-70-77

Vancouver

Matvienko DA, Kulemzin SV, Smagina AS, Belovezhets TN, Chikaev AN, Volkova OY et al. Analysis of in vitro activity of PSCA-specific CARs in the context of human NK cell line YT. Cellular Therapy and Transplantation. 2018 Jan 1;7(2):70-77. doi: 10.18620/ctt-1866-8836-2018-7-2-70-77

Author

Matvienko, Daria A. ; Kulemzin, Sergey V. ; Smagina, Anna S. et al. / Analysis of in vitro activity of PSCA-specific CARs in the context of human NK cell line YT. In: Cellular Therapy and Transplantation. 2018 ; Vol. 7, No. 2. pp. 70-77.

BibTeX

@article{d5cfa628e84d4673a2eae3879ae21036,

title = "Analysis of in vitro activity of PSCA-specific CARs in the context of human NK cell line YT",

abstract = "Comprehensive structural optimization of chimeric antigen receptors (CARs) is the key to their successful performance both in vitro and in vivo. In order to compare various CAR designs in an unified format, we took advantage of a lentiviral platform, where all CAR modules can be easily shuffled and tested for functionality. This platform was used to delineate the effects of various spacer regions on the function of a PSCA-specific CAR in the context of a human NK cell line, YT. We show that three CAR designs (IgG1-, CD8a-, and spacerless) perform similarly in vitro regardless of the length of the spacer region.",

keywords = "Adoptive immunotherapy, Chimeric antigen receptor (CAR), Prostate cancer, Prostate stem cell antigen (PSCA)",

author = "Matvienko, {Daria A.} and Kulemzin, {Sergey V.} and Smagina, {Anna S.} and Belovezhets, {Tatyana N.} and Chikaev, {Anton N.} and Volkova, {Olga Y.} and Chikaev, {Nikolay A.} and Koval, {Olga A.} and Kuligina, {Elena V.} and Taranin, {Alexandr V.} and Gorchakov, {Andrey A.}",

year = "2018",

month = jan,

day = "1",

doi = "10.18620/ctt-1866-8836-2018-7-2-70-77",

language = "English",

volume = "7",

pages = "70--77",

journal = "Cellular Therapy and Transplantation",

issn = "1867-416X",

publisher = "Universitatsklinikum Hamburg - Eppendorf",

number = "2",

}

RIS

TY - JOUR

T1 - Analysis of in vitro activity of PSCA-specific CARs in the context of human NK cell line YT

AU - Matvienko, Daria A.

AU - Kulemzin, Sergey V.

AU - Smagina, Anna S.

AU - Belovezhets, Tatyana N.

AU - Chikaev, Anton N.

AU - Volkova, Olga Y.

AU - Chikaev, Nikolay A.

AU - Koval, Olga A.

AU - Kuligina, Elena V.

AU - Taranin, Alexandr V.

AU - Gorchakov, Andrey A.

PY - 2018/1/1

Y1 - 2018/1/1

N2 - Comprehensive structural optimization of chimeric antigen receptors (CARs) is the key to their successful performance both in vitro and in vivo. In order to compare various CAR designs in an unified format, we took advantage of a lentiviral platform, where all CAR modules can be easily shuffled and tested for functionality. This platform was used to delineate the effects of various spacer regions on the function of a PSCA-specific CAR in the context of a human NK cell line, YT. We show that three CAR designs (IgG1-, CD8a-, and spacerless) perform similarly in vitro regardless of the length of the spacer region.

AB - Comprehensive structural optimization of chimeric antigen receptors (CARs) is the key to their successful performance both in vitro and in vivo. In order to compare various CAR designs in an unified format, we took advantage of a lentiviral platform, where all CAR modules can be easily shuffled and tested for functionality. This platform was used to delineate the effects of various spacer regions on the function of a PSCA-specific CAR in the context of a human NK cell line, YT. We show that three CAR designs (IgG1-, CD8a-, and spacerless) perform similarly in vitro regardless of the length of the spacer region.

KW - Adoptive immunotherapy

KW - Chimeric antigen receptor (CAR)

KW - Prostate cancer

KW - Prostate stem cell antigen (PSCA)

UR - http://www.scopus.com/inward/record.url?scp=85055341900&partnerID=8YFLogxK

U2 - 10.18620/ctt-1866-8836-2018-7-2-70-77

DO - 10.18620/ctt-1866-8836-2018-7-2-70-77

M3 - Article

AN - SCOPUS:85055341900

VL - 7

SP - 70

EP - 77

JO - Cellular Therapy and Transplantation

JF - Cellular Therapy and Transplantation

SN - 1867-416X

IS - 2

ER -

ID: 17249720