Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
Acute blood loss in mice forces differentiation of both CD45-positive and CD45-negative erythroid cells and leads to a decreased CCL3 chemokine production by bone marrow erythroid cells. / Nazarov, Kirill; Perik-Zavodskii, Roman; Perik-Zavodskaia, Olga и др.
в: PLoS ONE, Том 19, № 9, e0309455, 09.2024.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - Acute blood loss in mice forces differentiation of both CD45-positive and CD45-negative erythroid cells and leads to a decreased CCL3 chemokine production by bone marrow erythroid cells
AU - Nazarov, Kirill
AU - Perik-Zavodskii, Roman
AU - Perik-Zavodskaia, Olga
AU - Alrhmoun, Saleh
AU - Volynets, Marina
AU - Shevchenko, Julia
AU - Sennikov, Sergey
N1 - This research was funded by Ministry of Higher Education and Science, State Assignment No. 0415-2024-0012 awarded to SS. The specific roles of this author are articulated in the ‘author contributions’ section. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Copyright: © 2024 Nazarov et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2024/9
Y1 - 2024/9
N2 - Hemorrhage, a condition that accompanies most physical trauma cases, remains an important field of study, a field that has been extensively studied in the immunological context for myeloid and lymphoid cells, but not as much for erythroid cells. In this study, we studied the immunological response of murine erythroid cells to acute blood loss using flow cytometry, NanoString immune transcriptome profiling, and BioPlex cytokine secretome profiling. We observed that acute blood loss forces the differentiation of murine erythroid cells in both bone marrow and spleen and that there was an up-regulation of several immune response genes, in particular pathogen-associated molecular pattern sensing gene Clec5a in post-acute blood loss murine bone marrow erythroid cells. We believe that the up-regulation of the Clec5a gene in bone marrow erythroid cells could help bone marrow erythroid cells detect and eliminate pathogens with the help of reactive oxygen species and antimicrobial proteins calprotectin and cathelicidin, the genes of which (S100a8, S100a9, and Camp) dominate the expression in bone marrow erythroid cells of mice.
AB - Hemorrhage, a condition that accompanies most physical trauma cases, remains an important field of study, a field that has been extensively studied in the immunological context for myeloid and lymphoid cells, but not as much for erythroid cells. In this study, we studied the immunological response of murine erythroid cells to acute blood loss using flow cytometry, NanoString immune transcriptome profiling, and BioPlex cytokine secretome profiling. We observed that acute blood loss forces the differentiation of murine erythroid cells in both bone marrow and spleen and that there was an up-regulation of several immune response genes, in particular pathogen-associated molecular pattern sensing gene Clec5a in post-acute blood loss murine bone marrow erythroid cells. We believe that the up-regulation of the Clec5a gene in bone marrow erythroid cells could help bone marrow erythroid cells detect and eliminate pathogens with the help of reactive oxygen species and antimicrobial proteins calprotectin and cathelicidin, the genes of which (S100a8, S100a9, and Camp) dominate the expression in bone marrow erythroid cells of mice.
KW - Animals
KW - Mice
KW - Erythroid Cells/metabolism
KW - Cell Differentiation
KW - Chemokine CCL3/metabolism
KW - Leukocyte Common Antigens/metabolism
KW - Lectins, C-Type/metabolism
KW - Mice, Inbred C57BL
KW - Calgranulin A/metabolism
KW - Bone Marrow Cells/metabolism
KW - Calgranulin B/metabolism
KW - Male
UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85203328742&origin=inward&txGid=24c9aa07eb3c2d62ae35a503c7b81d81
UR - https://www.mendeley.com/catalogue/8f11601d-ecab-3991-bd9d-242f07e801ed/
U2 - 10.1371/journal.pone.0309455
DO - 10.1371/journal.pone.0309455
M3 - Article
C2 - 39231178
VL - 19
JO - PLoS ONE
JF - PLoS ONE
SN - 1932-6203
IS - 9
M1 - e0309455
ER -
ID: 60723861