Acute blood loss in mice forces differentiation of both CD45-positive and CD45-negative erythroid cells and leads to a decreased CCL3 chemokine production by bone marrow erythroid cells

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Acute blood loss in mice forces differentiation of both CD45-positive and CD45-negative erythroid cells and leads to a decreased CCL3 chemokine production by bone marrow erythroid cells. / Nazarov, Kirill; Perik-Zavodskii, Roman; Perik-Zavodskaia, Olga et al.

In: PLoS ONE, Vol. 19, No. 9, e0309455, 09.2024.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{41572f4f85364fcdb441393d9ac293a9,

title = "Acute blood loss in mice forces differentiation of both CD45-positive and CD45-negative erythroid cells and leads to a decreased CCL3 chemokine production by bone marrow erythroid cells",

abstract = "Hemorrhage, a condition that accompanies most physical trauma cases, remains an important field of study, a field that has been extensively studied in the immunological context for myeloid and lymphoid cells, but not as much for erythroid cells. In this study, we studied the immunological response of murine erythroid cells to acute blood loss using flow cytometry, NanoString immune transcriptome profiling, and BioPlex cytokine secretome profiling. We observed that acute blood loss forces the differentiation of murine erythroid cells in both bone marrow and spleen and that there was an up-regulation of several immune response genes, in particular pathogen-associated molecular pattern sensing gene Clec5a in post-acute blood loss murine bone marrow erythroid cells. We believe that the up-regulation of the Clec5a gene in bone marrow erythroid cells could help bone marrow erythroid cells detect and eliminate pathogens with the help of reactive oxygen species and antimicrobial proteins calprotectin and cathelicidin, the genes of which (S100a8, S100a9, and Camp) dominate the expression in bone marrow erythroid cells of mice.",

keywords = "Animals, Mice, Erythroid Cells/metabolism, Cell Differentiation, Chemokine CCL3/metabolism, Leukocyte Common Antigens/metabolism, Lectins, C-Type/metabolism, Mice, Inbred C57BL, Calgranulin A/metabolism, Bone Marrow Cells/metabolism, Calgranulin B/metabolism, Male",

author = "Kirill Nazarov and Roman Perik-Zavodskii and Olga Perik-Zavodskaia and Saleh Alrhmoun and Marina Volynets and Julia Shevchenko and Sergey Sennikov",

note = "This research was funded by Ministry of Higher Education and Science, State Assignment No. 0415-2024-0012 awarded to SS. The specific roles of this author are articulated in the {\textquoteleft}author contributions{\textquoteright} section. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Copyright: {\textcopyright} 2024 Nazarov et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.",

year = "2024",

month = sep,

doi = "10.1371/journal.pone.0309455",

language = "English",

volume = "19",

journal = "PLoS ONE",

issn = "1932-6203",

publisher = "Public Library of Science",

number = "9",

}

RIS

TY - JOUR

T1 - Acute blood loss in mice forces differentiation of both CD45-positive and CD45-negative erythroid cells and leads to a decreased CCL3 chemokine production by bone marrow erythroid cells

AU - Nazarov, Kirill

AU - Perik-Zavodskii, Roman

AU - Perik-Zavodskaia, Olga

AU - Alrhmoun, Saleh

AU - Volynets, Marina

AU - Shevchenko, Julia

AU - Sennikov, Sergey

N1 - This research was funded by Ministry of Higher Education and Science, State Assignment No. 0415-2024-0012 awarded to SS. The specific roles of this author are articulated in the ‘author contributions’ section. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript. Copyright: © 2024 Nazarov et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.

PY - 2024/9

Y1 - 2024/9

N2 - Hemorrhage, a condition that accompanies most physical trauma cases, remains an important field of study, a field that has been extensively studied in the immunological context for myeloid and lymphoid cells, but not as much for erythroid cells. In this study, we studied the immunological response of murine erythroid cells to acute blood loss using flow cytometry, NanoString immune transcriptome profiling, and BioPlex cytokine secretome profiling. We observed that acute blood loss forces the differentiation of murine erythroid cells in both bone marrow and spleen and that there was an up-regulation of several immune response genes, in particular pathogen-associated molecular pattern sensing gene Clec5a in post-acute blood loss murine bone marrow erythroid cells. We believe that the up-regulation of the Clec5a gene in bone marrow erythroid cells could help bone marrow erythroid cells detect and eliminate pathogens with the help of reactive oxygen species and antimicrobial proteins calprotectin and cathelicidin, the genes of which (S100a8, S100a9, and Camp) dominate the expression in bone marrow erythroid cells of mice.

AB - Hemorrhage, a condition that accompanies most physical trauma cases, remains an important field of study, a field that has been extensively studied in the immunological context for myeloid and lymphoid cells, but not as much for erythroid cells. In this study, we studied the immunological response of murine erythroid cells to acute blood loss using flow cytometry, NanoString immune transcriptome profiling, and BioPlex cytokine secretome profiling. We observed that acute blood loss forces the differentiation of murine erythroid cells in both bone marrow and spleen and that there was an up-regulation of several immune response genes, in particular pathogen-associated molecular pattern sensing gene Clec5a in post-acute blood loss murine bone marrow erythroid cells. We believe that the up-regulation of the Clec5a gene in bone marrow erythroid cells could help bone marrow erythroid cells detect and eliminate pathogens with the help of reactive oxygen species and antimicrobial proteins calprotectin and cathelicidin, the genes of which (S100a8, S100a9, and Camp) dominate the expression in bone marrow erythroid cells of mice.

KW - Animals

KW - Mice

KW - Erythroid Cells/metabolism

KW - Cell Differentiation

KW - Chemokine CCL3/metabolism

KW - Leukocyte Common Antigens/metabolism

KW - Lectins, C-Type/metabolism

KW - Mice, Inbred C57BL

KW - Calgranulin A/metabolism

KW - Bone Marrow Cells/metabolism

KW - Calgranulin B/metabolism

KW - Male

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85203328742&origin=inward&txGid=24c9aa07eb3c2d62ae35a503c7b81d81

UR - https://www.mendeley.com/catalogue/8f11601d-ecab-3991-bd9d-242f07e801ed/

U2 - 10.1371/journal.pone.0309455

DO - 10.1371/journal.pone.0309455

M3 - Article

C2 - 39231178

VL - 19

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 9

M1 - e0309455

ER -

ID: 60723861