TY - JOUR

T1 - A subpopulation of human bone marrow erythroid cells displays a myeloid gene expression signature similar to that of classic monocytes

AU - Perik-Zavodskii, Roman

AU - Perik-Zavodskaia, Olga

AU - Shevchenko, Julia

AU - Volynets, Marina

AU - Alrhmoun, Saleh

AU - Nazarov, Kirill

AU - Denisova, Vera

AU - Sennikov, Sergey

PY - 2024/7

Y1 - 2024/7

N2 - Erythroid cells, serving as progenitors and precursors to erythrocytes responsible for oxygen transport, were shown to exhibit an immunosuppressive and immunoregulatory phenotype. Previous investigations from our research group have revealed an antimicrobial gene expression profile within murine bone marrow erythroid cells which suggested a role for erythroid cells in innate immunity. In the present study, we focused on elucidating the characteristics of human bone marrow erythroid cells through comprehensive analyses, including NanoString gene expression profiling utilizing the Immune Response V2 panel, a BioPlex examination of chemokine and TGF-beta family proteins secretion, and analysis of publicly available single-cell RNA-seq data. Our findings demonstrate that an erythroid cell subpopulation manifests a myeloid-like gene expression signature comprised of antibacterial immunity and neutrophil chemotaxis genes which suggests an involvement of human erythroid cells in the innate immunity. Furthermore, we found that human erythroid cells secreted CCL22, CCL24, CXCL5, CXCL8, and MIF chemokines. The ability of human erythroid cells to express these chemokines might facilitate the restriction of immune cells in the bone marrow under normal conditions or contribute to the ability of erythroid cells to induce local immunosuppression by recruiting immune cells in their immediate vicinity in case of extramedullary hematopoiesis.

AB - Erythroid cells, serving as progenitors and precursors to erythrocytes responsible for oxygen transport, were shown to exhibit an immunosuppressive and immunoregulatory phenotype. Previous investigations from our research group have revealed an antimicrobial gene expression profile within murine bone marrow erythroid cells which suggested a role for erythroid cells in innate immunity. In the present study, we focused on elucidating the characteristics of human bone marrow erythroid cells through comprehensive analyses, including NanoString gene expression profiling utilizing the Immune Response V2 panel, a BioPlex examination of chemokine and TGF-beta family proteins secretion, and analysis of publicly available single-cell RNA-seq data. Our findings demonstrate that an erythroid cell subpopulation manifests a myeloid-like gene expression signature comprised of antibacterial immunity and neutrophil chemotaxis genes which suggests an involvement of human erythroid cells in the innate immunity. Furthermore, we found that human erythroid cells secreted CCL22, CCL24, CXCL5, CXCL8, and MIF chemokines. The ability of human erythroid cells to express these chemokines might facilitate the restriction of immune cells in the bone marrow under normal conditions or contribute to the ability of erythroid cells to induce local immunosuppression by recruiting immune cells in their immediate vicinity in case of extramedullary hematopoiesis.

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85199294827&origin=inward&txGid=659932ecdbb116807ebfb2db3feff096

UR - https://www.mendeley.com/catalogue/3e79e873-7586-31ef-8370-337d4955e3d6/

U2 - 10.1371/journal.pone.0305816

DO - 10.1371/journal.pone.0305816

M3 - Article

C2 - 39038020

VL - 19

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 7 July

M1 - e0305816

ER -