Research output: Contribution to journal › Article › peer-review
A subpopulation of human bone marrow erythroid cells displays a myeloid gene expression signature similar to that of classic monocytes. / Perik-Zavodskii, Roman; Perik-Zavodskaia, Olga; Shevchenko, Julia et al.
In: PLoS ONE, Vol. 19, No. 7 July, e0305816, 07.2024, p. e0305816.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - A subpopulation of human bone marrow erythroid cells displays a myeloid gene expression signature similar to that of classic monocytes
AU - Perik-Zavodskii, Roman
AU - Perik-Zavodskaia, Olga
AU - Shevchenko, Julia
AU - Volynets, Marina
AU - Alrhmoun, Saleh
AU - Nazarov, Kirill
AU - Denisova, Vera
AU - Sennikov, Sergey
N1 - Copyright: © 2024 Perik-Zavodskii et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2024/7
Y1 - 2024/7
N2 - Erythroid cells, serving as progenitors and precursors to erythrocytes responsible for oxygen transport, were shown to exhibit an immunosuppressive and immunoregulatory phenotype. Previous investigations from our research group have revealed an antimicrobial gene expression profile within murine bone marrow erythroid cells which suggested a role for erythroid cells in innate immunity. In the present study, we focused on elucidating the characteristics of human bone marrow erythroid cells through comprehensive analyses, including NanoString gene expression profiling utilizing the Immune Response V2 panel, a BioPlex examination of chemokine and TGF-beta family proteins secretion, and analysis of publicly available single-cell RNA-seq data. Our findings demonstrate that an erythroid cell subpopulation manifests a myeloid-like gene expression signature comprised of antibacterial immunity and neutrophil chemotaxis genes which suggests an involvement of human erythroid cells in the innate immunity. Furthermore, we found that human erythroid cells secreted CCL22, CCL24, CXCL5, CXCL8, and MIF chemokines. The ability of human erythroid cells to express these chemokines might facilitate the restriction of immune cells in the bone marrow under normal conditions or contribute to the ability of erythroid cells to induce local immunosuppression by recruiting immune cells in their immediate vicinity in case of extramedullary hematopoiesis.
AB - Erythroid cells, serving as progenitors and precursors to erythrocytes responsible for oxygen transport, were shown to exhibit an immunosuppressive and immunoregulatory phenotype. Previous investigations from our research group have revealed an antimicrobial gene expression profile within murine bone marrow erythroid cells which suggested a role for erythroid cells in innate immunity. In the present study, we focused on elucidating the characteristics of human bone marrow erythroid cells through comprehensive analyses, including NanoString gene expression profiling utilizing the Immune Response V2 panel, a BioPlex examination of chemokine and TGF-beta family proteins secretion, and analysis of publicly available single-cell RNA-seq data. Our findings demonstrate that an erythroid cell subpopulation manifests a myeloid-like gene expression signature comprised of antibacterial immunity and neutrophil chemotaxis genes which suggests an involvement of human erythroid cells in the innate immunity. Furthermore, we found that human erythroid cells secreted CCL22, CCL24, CXCL5, CXCL8, and MIF chemokines. The ability of human erythroid cells to express these chemokines might facilitate the restriction of immune cells in the bone marrow under normal conditions or contribute to the ability of erythroid cells to induce local immunosuppression by recruiting immune cells in their immediate vicinity in case of extramedullary hematopoiesis.
KW - Humans
KW - Monocytes/metabolism
KW - Erythroid Cells/metabolism
KW - Immunity, Innate
KW - Macrophage Migration-Inhibitory Factors/genetics
KW - Bone Marrow Cells/metabolism
KW - Transcriptome
KW - Gene Expression Profiling
KW - Chemokine CXCL5/metabolism
KW - Myeloid Cells/metabolism
KW - Chemokines/metabolism
KW - Interleukin-8
KW - Intramolecular Oxidoreductases
UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85199294827&origin=inward&txGid=659932ecdbb116807ebfb2db3feff096
UR - https://www.mendeley.com/catalogue/3e79e873-7586-31ef-8370-337d4955e3d6/
UR - http://10.1371/journal.pone.0305816
U2 - 10.1371/journal.pone.0305816
DO - 10.1371/journal.pone.0305816
M3 - Article
C2 - 39038020
VL - 19
SP - e0305816
JO - PLoS ONE
JF - PLoS ONE
SN - 1932-6203
IS - 7 July
M1 - e0305816
ER -
ID: 60863157