TY - JOUR

T1 - A Novel Aeromonas popoffii Phage AerP_220 Proposed to Be a Member of a New Tolavirus Genus in the Autographiviridae Family

AU - Morozova, Vera

AU - Kozlova, Yuliya

AU - Jdeed, Ghadeer

AU - Tikunov, Artem

AU - Ushakova, Tatyana

AU - Bardasheva, Alevtina

AU - Zhirakovskaia, Elena

AU - Poletaeva, Yuliya

AU - Ryabchikova, Elena

AU - Tikunova, Nina V

N1 - This study was supported by the Ministry of Science and Higher Education of Russian Federation within the project grant no. 075-15-2021-1085.

PY - 2022/12/7

Y1 - 2022/12/7

N2 - Aeromonas popoffii is one of the environmental Aeromonas species. A number of factors of virulence have been described for this species and it has been reported as a causative agent of urinary tract infection. The first A. popoffii bacteriophage AerP_220 along with its host strain A. popoffii CEMTC 4062 were isolated from river water. The phage has a podovirus morphotype, shows a narrow host range and is lytic against the host strain. The AerP_220 genome comprises 45,207 bp and does not contain genes responsible for antibiotic resistance and toxin production. Fifty-nine co-directional putative ORFs were found in the AerP_220 genome. Thirty-three ORFs encoded proteins with predicted functions; the products of 26 ORFs were hypothetical proteins. AerP_220 genome analysis revealed that this phage can be considered a novel species within the Autographiviridae family. Comparative genomic and proteomic analysis revealed that AerP_220 along with the Aeromonas phage vB_AspA_Tola (OM913599) are members of a new putative Tolavirus genus in the family Autographiviridae. The Gajwadongvirus and proposed Tolavirus genera along with Pantoea phage Nufs112 and phage Reminis could form a new Tolavirinae subfamily within the Autographiviridae family.

AB - Aeromonas popoffii is one of the environmental Aeromonas species. A number of factors of virulence have been described for this species and it has been reported as a causative agent of urinary tract infection. The first A. popoffii bacteriophage AerP_220 along with its host strain A. popoffii CEMTC 4062 were isolated from river water. The phage has a podovirus morphotype, shows a narrow host range and is lytic against the host strain. The AerP_220 genome comprises 45,207 bp and does not contain genes responsible for antibiotic resistance and toxin production. Fifty-nine co-directional putative ORFs were found in the AerP_220 genome. Thirty-three ORFs encoded proteins with predicted functions; the products of 26 ORFs were hypothetical proteins. AerP_220 genome analysis revealed that this phage can be considered a novel species within the Autographiviridae family. Comparative genomic and proteomic analysis revealed that AerP_220 along with the Aeromonas phage vB_AspA_Tola (OM913599) are members of a new putative Tolavirus genus in the family Autographiviridae. The Gajwadongvirus and proposed Tolavirus genera along with Pantoea phage Nufs112 and phage Reminis could form a new Tolavirinae subfamily within the Autographiviridae family.

KW - Bacteriophages

KW - Proteomics

KW - Aeromonas/genetics

KW - Genomics

KW - Genome, Viral

KW - Phylogeny

KW - Tolavirus

KW - tail fiber

KW - bacteriophage

KW - comparative genomics

KW - proteomic analysis

KW - Aeromonas popoffii

KW - Autographiviridae

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85144527235&origin=inward&txGid=b24bc8349393bab71ca0da2717a5124a

UR - https://www.mendeley.com/catalogue/f1b2ce7b-e82c-35df-845b-e6441f2b6803/

U2 - 10.3390/v14122733

DO - 10.3390/v14122733

M3 - Article

C2 - 36560737

VL - 14

JO - Viruses

JF - Viruses

SN - 1999-4915

IS - 12

M1 - 2733

ER -