Research output: Contribution to journal › Article › peer-review
A Novel Aeromonas popoffii Phage AerP_220 Proposed to Be a Member of a New Tolavirus Genus in the Autographiviridae Family. / Morozova, Vera; Kozlova, Yuliya; Jdeed, Ghadeer et al.
In: Viruses, Vol. 14, No. 12, 2733, 07.12.2022.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - A Novel Aeromonas popoffii Phage AerP_220 Proposed to Be a Member of a New Tolavirus Genus in the Autographiviridae Family
AU - Morozova, Vera
AU - Kozlova, Yuliya
AU - Jdeed, Ghadeer
AU - Tikunov, Artem
AU - Ushakova, Tatyana
AU - Bardasheva, Alevtina
AU - Zhirakovskaia, Elena
AU - Poletaeva, Yuliya
AU - Ryabchikova, Elena
AU - Tikunova, Nina V
N1 - This study was supported by the Ministry of Science and Higher Education of Russian Federation within the project grant no. 075-15-2021-1085.
PY - 2022/12/7
Y1 - 2022/12/7
N2 - Aeromonas popoffii is one of the environmental Aeromonas species. A number of factors of virulence have been described for this species and it has been reported as a causative agent of urinary tract infection. The first A. popoffii bacteriophage AerP_220 along with its host strain A. popoffii CEMTC 4062 were isolated from river water. The phage has a podovirus morphotype, shows a narrow host range and is lytic against the host strain. The AerP_220 genome comprises 45,207 bp and does not contain genes responsible for antibiotic resistance and toxin production. Fifty-nine co-directional putative ORFs were found in the AerP_220 genome. Thirty-three ORFs encoded proteins with predicted functions; the products of 26 ORFs were hypothetical proteins. AerP_220 genome analysis revealed that this phage can be considered a novel species within the Autographiviridae family. Comparative genomic and proteomic analysis revealed that AerP_220 along with the Aeromonas phage vB_AspA_Tola (OM913599) are members of a new putative Tolavirus genus in the family Autographiviridae. The Gajwadongvirus and proposed Tolavirus genera along with Pantoea phage Nufs112 and phage Reminis could form a new Tolavirinae subfamily within the Autographiviridae family.
AB - Aeromonas popoffii is one of the environmental Aeromonas species. A number of factors of virulence have been described for this species and it has been reported as a causative agent of urinary tract infection. The first A. popoffii bacteriophage AerP_220 along with its host strain A. popoffii CEMTC 4062 were isolated from river water. The phage has a podovirus morphotype, shows a narrow host range and is lytic against the host strain. The AerP_220 genome comprises 45,207 bp and does not contain genes responsible for antibiotic resistance and toxin production. Fifty-nine co-directional putative ORFs were found in the AerP_220 genome. Thirty-three ORFs encoded proteins with predicted functions; the products of 26 ORFs were hypothetical proteins. AerP_220 genome analysis revealed that this phage can be considered a novel species within the Autographiviridae family. Comparative genomic and proteomic analysis revealed that AerP_220 along with the Aeromonas phage vB_AspA_Tola (OM913599) are members of a new putative Tolavirus genus in the family Autographiviridae. The Gajwadongvirus and proposed Tolavirus genera along with Pantoea phage Nufs112 and phage Reminis could form a new Tolavirinae subfamily within the Autographiviridae family.
KW - Bacteriophages
KW - Proteomics
KW - Aeromonas/genetics
KW - Genomics
KW - Genome, Viral
KW - Phylogeny
KW - Tolavirus
KW - tail fiber
KW - bacteriophage
KW - comparative genomics
KW - proteomic analysis
KW - Aeromonas popoffii
KW - Autographiviridae
UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85144527235&origin=inward&txGid=b24bc8349393bab71ca0da2717a5124a
UR - https://www.mendeley.com/catalogue/f1b2ce7b-e82c-35df-845b-e6441f2b6803/
U2 - 10.3390/v14122733
DO - 10.3390/v14122733
M3 - Article
C2 - 36560737
VL - 14
JO - Viruses
JF - Viruses
SN - 1999-4915
IS - 12
M1 - 2733
ER -
ID: 42566439