Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
A combined banding method that allows the reliable identification of chromosomes as well as differentiation of AT- and GC-rich heterochromatin. / Lemskaya, Natalya A.; Kulemzina, Anastasia I.; Beklemisheva, Violetta R. и др.
в: Chromosome Research, Том 26, № 4, 01.12.2018, стр. 307-315.Результаты исследований: Научные публикации в периодических изданиях › статья › Рецензирование
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TY - JOUR
T1 - A combined banding method that allows the reliable identification of chromosomes as well as differentiation of AT- and GC-rich heterochromatin
AU - Lemskaya, Natalya A.
AU - Kulemzina, Anastasia I.
AU - Beklemisheva, Violetta R.
AU - Biltueva, Larisa S.
AU - Proskuryakova, Anastasia A.
AU - Hallenbeck, John M.
AU - Perelman, Polina L.
AU - Graphodatsky, Alexander S.
N1 - Publisher Copyright: © 2018, Springer Nature B.V.
PY - 2018/12/1
Y1 - 2018/12/1
N2 - Сonstitutive heterochromatin areas are revealed by differential staining as C-positive chromosomal regions. These C-positive bands may greatly vary by location, size, and nucleotide composition. CBG-banding is the most commonly used method to detect structural heterochromatin in animals. The difficulty in identification of individual chromosomes represents an unresolved problem of this method as the body of the chromosome is stained uniformly and does not have banding pattern beyond C-bands. Here, we present the method that we called CDAG for sequential heterochromatin staining after differential GTG-banding. The method uses G-banding followed by heat denaturation in the presence of formamide with consecutive fluorochrome staining. The new technique is valid for the concurrent revealing of heterochromatin position due to differential banding of chromosomes and heterochromatin composition (AT-/GC-rich) in animal karyotyping.
AB - Сonstitutive heterochromatin areas are revealed by differential staining as C-positive chromosomal regions. These C-positive bands may greatly vary by location, size, and nucleotide composition. CBG-banding is the most commonly used method to detect structural heterochromatin in animals. The difficulty in identification of individual chromosomes represents an unresolved problem of this method as the body of the chromosome is stained uniformly and does not have banding pattern beyond C-bands. Here, we present the method that we called CDAG for sequential heterochromatin staining after differential GTG-banding. The method uses G-banding followed by heat denaturation in the presence of formamide with consecutive fluorochrome staining. The new technique is valid for the concurrent revealing of heterochromatin position due to differential banding of chromosomes and heterochromatin composition (AT-/GC-rich) in animal karyotyping.
KW - AT-rich
KW - C-banding
KW - Chromosome
KW - Differential staining
KW - G-banding
KW - GC-rich
KW - Heterochromatin composition
KW - Karyotype
KW - Сonstitutive heterochromatin
KW - LOCALIZATION
KW - X-CHROMOSOME
KW - CHROMATIN
KW - CALF
KW - Constitutive heterochromatin
KW - DAPI
KW - MICROTUS
KW - EVOLUTION
KW - MOUSE SATELLITE DNA
KW - FLUORESCENCE
KW - DIPI
UR - http://www.scopus.com/inward/record.url?scp=85056798864&partnerID=8YFLogxK
U2 - 10.1007/s10577-018-9589-9
DO - 10.1007/s10577-018-9589-9
M3 - Article
C2 - 30443803
AN - SCOPUS:85056798864
VL - 26
SP - 307
EP - 315
JO - Chromosome Research
JF - Chromosome Research
SN - 0967-3849
IS - 4
ER -
ID: 17487907