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A combined banding method that allows the reliable identification of chromosomes as well as differentiation of AT- and GC-rich heterochromatin. / Lemskaya, Natalya A.; Kulemzina, Anastasia I.; Beklemisheva, Violetta R. et al.

In: Chromosome Research, Vol. 26, No. 4, 01.12.2018, p. 307-315.

Research output: Contribution to journal › Article › peer-review

Harvard

Lemskaya, NA, Kulemzina, AI, Beklemisheva, VR, Biltueva, LS, Proskuryakova, AA, Hallenbeck, JM, Perelman, PL & Graphodatsky, AS 2018, 'A combined banding method that allows the reliable identification of chromosomes as well as differentiation of AT- and GC-rich heterochromatin', Chromosome Research, vol. 26, no. 4, pp. 307-315. https://doi.org/10.1007/s10577-018-9589-9

APA

Lemskaya, N. A., Kulemzina, A. I., Beklemisheva, V. R., Biltueva, L. S., Proskuryakova, A. A., Hallenbeck, J. M., Perelman, P. L., & Graphodatsky, A. S. (2018). A combined banding method that allows the reliable identification of chromosomes as well as differentiation of AT- and GC-rich heterochromatin. Chromosome Research, 26(4), 307-315. https://doi.org/10.1007/s10577-018-9589-9

Vancouver

Lemskaya NA, Kulemzina AI, Beklemisheva VR, Biltueva LS, Proskuryakova AA, Hallenbeck JM et al. A combined banding method that allows the reliable identification of chromosomes as well as differentiation of AT- and GC-rich heterochromatin. Chromosome Research. 2018 Dec 1;26(4):307-315. doi: 10.1007/s10577-018-9589-9

Author

Lemskaya, Natalya A. ; Kulemzina, Anastasia I. ; Beklemisheva, Violetta R. et al. / A combined banding method that allows the reliable identification of chromosomes as well as differentiation of AT- and GC-rich heterochromatin. In: Chromosome Research. 2018 ; Vol. 26, No. 4. pp. 307-315.

BibTeX

@article{07be8ecfc59140fead1901dbba467dd3,
title = "A combined banding method that allows the reliable identification of chromosomes as well as differentiation of AT- and GC-rich heterochromatin",
abstract = "Сonstitutive heterochromatin areas are revealed by differential staining as C-positive chromosomal regions. These C-positive bands may greatly vary by location, size, and nucleotide composition. CBG-banding is the most commonly used method to detect structural heterochromatin in animals. The difficulty in identification of individual chromosomes represents an unresolved problem of this method as the body of the chromosome is stained uniformly and does not have banding pattern beyond C-bands. Here, we present the method that we called CDAG for sequential heterochromatin staining after differential GTG-banding. The method uses G-banding followed by heat denaturation in the presence of formamide with consecutive fluorochrome staining. The new technique is valid for the concurrent revealing of heterochromatin position due to differential banding of chromosomes and heterochromatin composition (AT-/GC-rich) in animal karyotyping.",
keywords = "AT-rich, C-banding, Chromosome, Differential staining, G-banding, GC-rich, Heterochromatin composition, Karyotype, Сonstitutive heterochromatin, LOCALIZATION, X-CHROMOSOME, CHROMATIN, CALF, Constitutive heterochromatin, DAPI, MICROTUS, EVOLUTION, MOUSE SATELLITE DNA, FLUORESCENCE, DIPI",
author = "Lemskaya, {Natalya A.} and Kulemzina, {Anastasia I.} and Beklemisheva, {Violetta R.} and Biltueva, {Larisa S.} and Proskuryakova, {Anastasia A.} and Hallenbeck, {John M.} and Perelman, {Polina L.} and Graphodatsky, {Alexander S.}",
note = "Publisher Copyright: {\textcopyright} 2018, Springer Nature B.V.",
year = "2018",
month = dec,
day = "1",
doi = "10.1007/s10577-018-9589-9",
language = "English",
volume = "26",
pages = "307--315",
journal = "Chromosome Research",
issn = "0967-3849",
publisher = "Springer Netherlands",
number = "4",

}

RIS

TY - JOUR

T1 - A combined banding method that allows the reliable identification of chromosomes as well as differentiation of AT- and GC-rich heterochromatin

AU - Lemskaya, Natalya A.

AU - Kulemzina, Anastasia I.

AU - Beklemisheva, Violetta R.

AU - Biltueva, Larisa S.

AU - Proskuryakova, Anastasia A.

AU - Hallenbeck, John M.

AU - Perelman, Polina L.

AU - Graphodatsky, Alexander S.

N1 - Publisher Copyright: © 2018, Springer Nature B.V.

PY - 2018/12/1

Y1 - 2018/12/1

N2 - Сonstitutive heterochromatin areas are revealed by differential staining as C-positive chromosomal regions. These C-positive bands may greatly vary by location, size, and nucleotide composition. CBG-banding is the most commonly used method to detect structural heterochromatin in animals. The difficulty in identification of individual chromosomes represents an unresolved problem of this method as the body of the chromosome is stained uniformly and does not have banding pattern beyond C-bands. Here, we present the method that we called CDAG for sequential heterochromatin staining after differential GTG-banding. The method uses G-banding followed by heat denaturation in the presence of formamide with consecutive fluorochrome staining. The new technique is valid for the concurrent revealing of heterochromatin position due to differential banding of chromosomes and heterochromatin composition (AT-/GC-rich) in animal karyotyping.

AB - Сonstitutive heterochromatin areas are revealed by differential staining as C-positive chromosomal regions. These C-positive bands may greatly vary by location, size, and nucleotide composition. CBG-banding is the most commonly used method to detect structural heterochromatin in animals. The difficulty in identification of individual chromosomes represents an unresolved problem of this method as the body of the chromosome is stained uniformly and does not have banding pattern beyond C-bands. Here, we present the method that we called CDAG for sequential heterochromatin staining after differential GTG-banding. The method uses G-banding followed by heat denaturation in the presence of formamide with consecutive fluorochrome staining. The new technique is valid for the concurrent revealing of heterochromatin position due to differential banding of chromosomes and heterochromatin composition (AT-/GC-rich) in animal karyotyping.

KW - AT-rich

KW - C-banding

KW - Chromosome

KW - Differential staining

KW - G-banding

KW - GC-rich

KW - Heterochromatin composition

KW - Karyotype

KW - Сonstitutive heterochromatin

KW - LOCALIZATION

KW - X-CHROMOSOME

KW - CHROMATIN

KW - CALF

KW - Constitutive heterochromatin

KW - DAPI

KW - MICROTUS

KW - EVOLUTION

KW - MOUSE SATELLITE DNA

KW - FLUORESCENCE

KW - DIPI

UR - http://www.scopus.com/inward/record.url?scp=85056798864&partnerID=8YFLogxK

U2 - 10.1007/s10577-018-9589-9

DO - 10.1007/s10577-018-9589-9

M3 - Article

C2 - 30443803

AN - SCOPUS:85056798864

VL - 26

SP - 307

EP - 315

JO - Chromosome Research

JF - Chromosome Research

SN - 0967-3849

IS - 4

ER -

ID: 17487907