TY - JOUR

T1 - Two-stage probe atomization GFAAS for direct determination of trace Cd and Pb in whole bovine blood

AU - Volzhenin, Artyom V.

AU - Petrova, Natalya I.

AU - Skiba, Tatyana V.

AU - Saprykin, Anatoly I.

PY - 2018/9/1

Y1 - 2018/9/1

N2 - Graphite furnace atomic absorption spectrometry (GFAAS) is rarely used for direct trace analysis of whole blood because of strong interferences caused by the organic matrix. Especially it concerns bovine blood samples, containing more proteinaceous and lipid components in comparison with human blood. In our work, the novel two-stage probe atomization (TPA) technique for Cd and Pb determination by GFAAS in whole bovine blood with minimum sample pre-treatment has been developed. Making use of TPA and chemical modifier has eliminated matrix suppression and decreased the non-selective absorption level by several times. It allowed us to dispense with brief sample pre-treatment by a mixture of nitric acid and hydrogen peroxide (1:1) without autoclave digestion. The pyrolysis temperatures were 650 °C for Cd and 900 °C for Pb in the presence of Pd modifier. The analytical signal dependence on the number of concentrating cycles has been studied. The maximal number of capturing cycles was found (15 and 10 for Cd and Pb). Applying of multiple probe concentrating has additionally decreased the limits of detection (LODs) comparing with single TPA. The achievable LODs in optimized conditions were found to be 0.01 and 0.2 μg/L for Cd and Pb, respectively (3 s test). The accuracy of the proposed technique was confirmed by anodic stripping voltammetry (ASV). The relative standard deviation for 5.1 μg/L Cd and 26 μg/L Pb concentrations was 6.4% and 7.5%, respectively.

AB - Graphite furnace atomic absorption spectrometry (GFAAS) is rarely used for direct trace analysis of whole blood because of strong interferences caused by the organic matrix. Especially it concerns bovine blood samples, containing more proteinaceous and lipid components in comparison with human blood. In our work, the novel two-stage probe atomization (TPA) technique for Cd and Pb determination by GFAAS in whole bovine blood with minimum sample pre-treatment has been developed. Making use of TPA and chemical modifier has eliminated matrix suppression and decreased the non-selective absorption level by several times. It allowed us to dispense with brief sample pre-treatment by a mixture of nitric acid and hydrogen peroxide (1:1) without autoclave digestion. The pyrolysis temperatures were 650 °C for Cd and 900 °C for Pb in the presence of Pd modifier. The analytical signal dependence on the number of concentrating cycles has been studied. The maximal number of capturing cycles was found (15 and 10 for Cd and Pb). Applying of multiple probe concentrating has additionally decreased the limits of detection (LODs) comparing with single TPA. The achievable LODs in optimized conditions were found to be 0.01 and 0.2 μg/L for Cd and Pb, respectively (3 s test). The accuracy of the proposed technique was confirmed by anodic stripping voltammetry (ASV). The relative standard deviation for 5.1 μg/L Cd and 26 μg/L Pb concentrations was 6.4% and 7.5%, respectively.

KW - ATOMIC-ABSORPTION-SPECTROMETRY

KW - PLASMA-MASS SPECTROMETRY

KW - X-RAY-FLUORESCENCE

KW - BIOLOGICAL SAMPLES

KW - ICP-MS

KW - CADMIUM

KW - LEAD

KW - ELEMENTS

KW - QUANTIFICATION

KW - PALLADIUM

UR - http://www.scopus.com/inward/record.url?scp=85047250496&partnerID=8YFLogxK

U2 - 10.1016/j.microc.2018.05.033

DO - 10.1016/j.microc.2018.05.033

M3 - Article

AN - SCOPUS:85047250496

VL - 141

SP - 210

EP - 214

JO - Microchemical Journal

JF - Microchemical Journal

SN - 0026-265X

ER -