Research output: Contribution to journal › Article › peer-review
Triplet Fullerenes as Prospective Spin Labels for Nanoscale Distance Measurements by Pulsed Dipolar EPR Spectroscopy. / Krumkacheva, Olesya A.; Timofeev, Ivan O.; Politanskaya, Larisa V. et al.
In: Angewandte Chemie - International Edition, Vol. 58, No. 38, 12.08.2019, p. 13271-13275.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Triplet Fullerenes as Prospective Spin Labels for Nanoscale Distance Measurements by Pulsed Dipolar EPR Spectroscopy
AU - Krumkacheva, Olesya A.
AU - Timofeev, Ivan O.
AU - Politanskaya, Larisa V.
AU - Polienko, Yuliya F.
AU - Tretyakov, Evgeny V.
AU - Rogozhnikova, Olga Yu
AU - Trukhin, Dmitry V.
AU - Tormyshev, Victor M.
AU - Chubarov, Alexey S.
AU - Bagryanskaya, Elena G.
AU - Fedin, Matvey V.
N1 - Publisher Copyright: © 2019 Wiley-VCH Verlag GmbH & Co. KGaA, Weinheim
PY - 2019/8/12
Y1 - 2019/8/12
N2 - Precise nanoscale distance measurements by pulsed electron paramagnetic resonance (EPR) spectroscopy play a crucial role in structural studies of biomolecules. The properties of the spin labels used in this approach determine the sensitivity limits, attainable distances, and proximity to biological conditions. Herein, we propose and validate the use of photoexcited fullerenes as spin labels for pulsed dipolar (PD) EPR distance measurements. Hyperpolarization and the narrower spectrum of fullerenes compared to other triplets (e.g., porphyrins) boost the sensitivity, and superior relaxation properties allow PD EPR measurements up to a near-room temperature. This approach is demonstrated using fullerene–nitroxide and fullerene–triarylmethyl pairs, as well as a supramolecular complex of fullerene with nitroxide-labeled protein. Photoexcited triplet fullerenes can be considered as new spin labels with outstanding spectroscopic properties for future structural studies of biomolecules.
AB - Precise nanoscale distance measurements by pulsed electron paramagnetic resonance (EPR) spectroscopy play a crucial role in structural studies of biomolecules. The properties of the spin labels used in this approach determine the sensitivity limits, attainable distances, and proximity to biological conditions. Herein, we propose and validate the use of photoexcited fullerenes as spin labels for pulsed dipolar (PD) EPR distance measurements. Hyperpolarization and the narrower spectrum of fullerenes compared to other triplets (e.g., porphyrins) boost the sensitivity, and superior relaxation properties allow PD EPR measurements up to a near-room temperature. This approach is demonstrated using fullerene–nitroxide and fullerene–triarylmethyl pairs, as well as a supramolecular complex of fullerene with nitroxide-labeled protein. Photoexcited triplet fullerenes can be considered as new spin labels with outstanding spectroscopic properties for future structural studies of biomolecules.
KW - distance measurements
KW - EPR spectroscopy
KW - fullerenes
KW - PELDOR
KW - spin labels
KW - CELLS
KW - PROTEIN
KW - ACID
KW - HUMAN SERUM-ALBUMIN
KW - STATE
KW - ELDOR
KW - RADICALS
KW - C-60
KW - RANGE
UR - http://www.scopus.com/inward/record.url?scp=85070710781&partnerID=8YFLogxK
U2 - 10.1002/anie.201904152
DO - 10.1002/anie.201904152
M3 - Article
C2 - 31322814
AN - SCOPUS:85070710781
VL - 58
SP - 13271
EP - 13275
JO - Angewandte Chemie - International Edition
JF - Angewandte Chemie - International Edition
SN - 1433-7851
IS - 38
ER -
ID: 21238418