Research output: Contribution to journal › Article › peer-review
Triarylmethyl Radicals : An EPR Study of 13C Hyperfine Coupling Constants. / Kuzhelev, Andrey A.; Tormyshev, Victor M.; Rogozhnikova, Olga Yu et al.
In: Zeitschrift fur Physikalische Chemie, Vol. 231, No. 4, 01.04.2017, p. 777-794.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Triarylmethyl Radicals
T2 - An EPR Study of 13C Hyperfine Coupling Constants
AU - Kuzhelev, Andrey A.
AU - Tormyshev, Victor M.
AU - Rogozhnikova, Olga Yu
AU - Trukhin, Dmitry V.
AU - Troitskaya, Tatiana I.
AU - Strizhakov, Rodion K.
AU - Krumkacheva, Olesya A.
AU - Fedin, Matvey V.
AU - Bagryanskaya, Elena G.
N1 - Publisher Copyright: © 2017 Walter de Gruyter GmbH, Berlin/Boston 2017.
PY - 2017/4/1
Y1 - 2017/4/1
N2 - Triarylmethyl (TAM) radicals are widely used in electron paramagnetic resonance (EPR) spectroscopy as spin labels and in EPR imaging as spin probes for in vivo oxymetry. One of the key advantages of TAMs is the extremely narrow EPR line, especially in case of deuterated analogs (~2.5 μT). Another advantage is their slow spin relaxation even at physiological temperatures; in particular, this characteristic enables the use of pulsed dipolar EPR methods for distance measurements in biomolecules. In this study, a large series of TAM radicals and their deuterated analogs was synthesized, and the corresponding spectroscopic parameters including 13C hyperfine constants were determined for the first time. We observed negligible dependence of 13C hyperfine constants on the solvent or on the structure or number of substituents at para-C atoms of the aromatic rings. In addition, we demonstrated that 13C signals at natural abundance can be used for successful distance measurements at room temperature by pulsed electron double resonance (PELDOR or DEER).
AB - Triarylmethyl (TAM) radicals are widely used in electron paramagnetic resonance (EPR) spectroscopy as spin labels and in EPR imaging as spin probes for in vivo oxymetry. One of the key advantages of TAMs is the extremely narrow EPR line, especially in case of deuterated analogs (~2.5 μT). Another advantage is their slow spin relaxation even at physiological temperatures; in particular, this characteristic enables the use of pulsed dipolar EPR methods for distance measurements in biomolecules. In this study, a large series of TAM radicals and their deuterated analogs was synthesized, and the corresponding spectroscopic parameters including 13C hyperfine constants were determined for the first time. We observed negligible dependence of 13C hyperfine constants on the solvent or on the structure or number of substituents at para-C atoms of the aromatic rings. In addition, we demonstrated that 13C signals at natural abundance can be used for successful distance measurements at room temperature by pulsed electron double resonance (PELDOR or DEER).
KW - triarylmethyl radical
KW - DISTANCE MEASUREMENTS
KW - INTRACELLULAR OXYGEN PROBES
KW - ROOM-TEMPERATURE
KW - SPIN RELAXATION
KW - DIPOLAR SPECTROSCOPY
KW - DUAL-FUNCTION-PH
KW - ELECTRON-PARAMAGNETIC-RESONANCE
KW - LARGE-SCALE SYNTHESIS
KW - TRITYL RADICALS
KW - COMPREHENSIVE SOFTWARE PACKAGE
UR - http://www.scopus.com/inward/record.url?scp=85012298696&partnerID=8YFLogxK
U2 - 10.1515/zpch-2016-0811
DO - 10.1515/zpch-2016-0811
M3 - Article
C2 - 28539703
AN - SCOPUS:85012298696
VL - 231
SP - 777
EP - 794
JO - Zeitschrift fur Physikalische Chemie
JF - Zeitschrift fur Physikalische Chemie
SN - 0942-9352
IS - 4
ER -
ID: 9052688