Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Research › peer-review
Towards optical control of single blood platelet activation. / Spiryova, Darya V.; Karmatskih, Oleg Yu; Vorob'Ev, Alexei Yu et al.
SARATOV FALL MEETING 2017: LASER PHYSICS AND PHOTONICS XVIII; AND COMPUTATIONAL BIOPHYSICS AND ANALYSIS OF BIOMEDICAL DATA IV: Laser Physics and Photonics XVIII; and Computational Biophysics and Analysis of Biomedical Data IV. ed. / VL Derbov; DE Postnov. Vol. 10717 SPIE, 2018. 1071722 (Proceedings of SPIE; Vol. 10717).Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Research › peer-review
}
TY - GEN
T1 - Towards optical control of single blood platelet activation
AU - Spiryova, Darya V.
AU - Karmatskih, Oleg Yu
AU - Vorob'Ev, Alexei Yu
AU - Moskalensky, Alexander E.
N1 - Publisher Copyright: © 2018 SPIE.
PY - 2018/1/1
Y1 - 2018/1/1
N2 - Blood platelets play a pivotal role in blood coagulation and in other normal and pathological processes. The understanding of fundamental mechanisms underlying their functions is very important for diagnostics and treatment. Single-cell experiments are needed for this purpose, which are complicated by insufficient spatiotemporal precision of conventional activation protocols. We present an approach to trigger single platelet activation optically, without the need of reagent mixing. This is achieved using photolabile compound, which rapidly delivers epinephrine upon UV irradiation. We demonstrated the applicability of the technique to rapidly induce platelet activation for studying dynamics of activation. The presented method may give novel fundamental knowledge about platelet functions and facilitate current research of their ability to deliver drugs to tumors or vascular injury sites.
AB - Blood platelets play a pivotal role in blood coagulation and in other normal and pathological processes. The understanding of fundamental mechanisms underlying their functions is very important for diagnostics and treatment. Single-cell experiments are needed for this purpose, which are complicated by insufficient spatiotemporal precision of conventional activation protocols. We present an approach to trigger single platelet activation optically, without the need of reagent mixing. This is achieved using photolabile compound, which rapidly delivers epinephrine upon UV irradiation. We demonstrated the applicability of the technique to rapidly induce platelet activation for studying dynamics of activation. The presented method may give novel fundamental knowledge about platelet functions and facilitate current research of their ability to deliver drugs to tumors or vascular injury sites.
KW - Biophotonics
KW - Caged epinephrine
KW - Platelet activation
KW - Single cell optical control
KW - caged epinephrine
KW - biophotonics
KW - P2Y(1)
KW - SHAPE CHANGE
KW - single cell optical control
KW - MODEL
KW - platelet activation
UR - http://www.scopus.com/inward/record.url?scp=85047362362&partnerID=8YFLogxK
U2 - 10.1117/12.2305477
DO - 10.1117/12.2305477
M3 - Conference contribution
AN - SCOPUS:85047362362
VL - 10717
T3 - Proceedings of SPIE
BT - SARATOV FALL MEETING 2017: LASER PHYSICS AND PHOTONICS XVIII; AND COMPUTATIONAL BIOPHYSICS AND ANALYSIS OF BIOMEDICAL DATA IV
A2 - Derbov, VL
A2 - Postnov, DE
PB - SPIE
T2 - Saratov Fall Meeting 2017: Laser Physics and Photonics XVIII; and Computational Biophysics and Analysis of Biomedical Data IV
Y2 - 26 September 2017 through 30 September 2017
ER -
ID: 13541500