TY - JOUR

T1 - Structural Analysis of the LZTFL1 Protein by Principal Component Analysis (PCA-seq)

AU - Khegai, I. I.

AU - Yu, X.

AU - Efimov, V. M.

N1 - The work was financially supported from the budget of the Institute of Cytology and Genetics, Siberian Branch, Russian Academy of Sciences (project nos. FWNR-2022-0021 and FWNR-2022-0019).

PY - 2024/12

Y1 - 2024/12

N2 - Abstract: Objective: The single-nucleotide mutation rs17713054G>A in the promoter region of LZTFL1 (leucine zipper transcription factor like 1) gene is a factor in the severe course of coronavirus infection COVID-19. Structure of coded protein is not studied well still. Methods: We used the principal component analysis (PCA-seq) for computer statistical analysis of protein LZTFL1 aminoacid sequence. Results and Discussion: It is revealed that the presence of a high correlation between the first principal component of the translated amino acid sequence and eleven amino acid indices of the AA-index database, characterizing the physicochemical and biochemical properties of the protein. The indices BEGF750102, CHOP780209, PALJ810110, GEIM800107, QIAN880121, LEVM780102, PRAM900103 are associated with β-folding parameters. The LZTFL1 protein is part of the Bardet-Biedl Syndrome (BBS) protein complexes that regulate intracellular transport in the ciliated epithelium of the lungs. Conclusions: It is assumed that the presence of β-sheet elements in the structure of the LZTFL1 protein plays an important role in ACE2 receptor-mediated endocytosis, stimulating the rate of angiotensin-converting enzyme 2 recycling and accelerating the delivery of adherented coronavirus SARS-CoV-2 virions into the cell followed by the initiation of severe acute respiratory syndrome COVID-19.

AB - Abstract: Objective: The single-nucleotide mutation rs17713054G>A in the promoter region of LZTFL1 (leucine zipper transcription factor like 1) gene is a factor in the severe course of coronavirus infection COVID-19. Structure of coded protein is not studied well still. Methods: We used the principal component analysis (PCA-seq) for computer statistical analysis of protein LZTFL1 aminoacid sequence. Results and Discussion: It is revealed that the presence of a high correlation between the first principal component of the translated amino acid sequence and eleven amino acid indices of the AA-index database, characterizing the physicochemical and biochemical properties of the protein. The indices BEGF750102, CHOP780209, PALJ810110, GEIM800107, QIAN880121, LEVM780102, PRAM900103 are associated with β-folding parameters. The LZTFL1 protein is part of the Bardet-Biedl Syndrome (BBS) protein complexes that regulate intracellular transport in the ciliated epithelium of the lungs. Conclusions: It is assumed that the presence of β-sheet elements in the structure of the LZTFL1 protein plays an important role in ACE2 receptor-mediated endocytosis, stimulating the rate of angiotensin-converting enzyme 2 recycling and accelerating the delivery of adherented coronavirus SARS-CoV-2 virions into the cell followed by the initiation of severe acute respiratory syndrome COVID-19.

KW - ACE2 receptor

KW - BBS proteins

KW - LZTFL1 protein

KW - amino acid indices

KW - ciliary epithelium

KW - principal component analysis

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85212116066&origin=inward&txGid=1b3f42054e52ca248c924f3807f2d456

UR - https://www.mendeley.com/catalogue/06709c6f-a962-343d-bce9-d303aef0cf21/

U2 - 10.1134/S1068162024060244

DO - 10.1134/S1068162024060244

M3 - Article

VL - 50

SP - 2611

EP - 2617

JO - Russian Journal of Bioorganic Chemistry

JF - Russian Journal of Bioorganic Chemistry

SN - 1068-1620

IS - 6

ER -