Research output: Contribution to journal › Article › peer-review
Spatial specificity of auxin responses coordinates wood formation. / Brackmann, Klaus; Qi, Jiyan; Gebert, Michael et al.
In: Nature Communications, Vol. 9, No. 1, 875, 28.02.2018, p. 875.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Spatial specificity of auxin responses coordinates wood formation
AU - Brackmann, Klaus
AU - Qi, Jiyan
AU - Gebert, Michael
AU - Jouannet, Virginie
AU - Schlamp, Theresa
AU - Grünwald, Karin
AU - Wallner, Eva Sophie
AU - Novikova, Daria D.
AU - Levitsky, Victor G.
AU - Agustí, Javier
AU - Sanchez, Pablo
AU - Lohmann, Jan U.
AU - Greb, Thomas
N1 - Publisher Copyright: © 2018 The Author(s).
PY - 2018/2/28
Y1 - 2018/2/28
N2 - Spatial organization of signalling events of the phytohormone auxin is fundamental for maintaining a dynamic transition from plant stem cells to differentiated descendants. The cambium, the stem cell niche mediating wood formation, fundamentally depends on auxin signalling but its exact role and spatial organization is obscure. Here we show that, while auxin signalling levels increase in differentiating cambium descendants, a moderate level of signalling in cambial stem cells is essential for cambium activity. We identify the auxin-dependent transcription factor ARF5/MONOPTEROS to cell-autonomously restrict the number of stem cells by directly attenuating the activity of the stem cell-promoting WOX4 gene. In contrast, ARF3 and ARF4 function as cambium activators in a redundant fashion from outside of WOX4-expressing cells. Our results reveal an influence of auxin signalling on distinct cambium features by specific signalling components and allow the conceptual integration of plant stem cell systems with distinct anatomies.
AB - Spatial organization of signalling events of the phytohormone auxin is fundamental for maintaining a dynamic transition from plant stem cells to differentiated descendants. The cambium, the stem cell niche mediating wood formation, fundamentally depends on auxin signalling but its exact role and spatial organization is obscure. Here we show that, while auxin signalling levels increase in differentiating cambium descendants, a moderate level of signalling in cambial stem cells is essential for cambium activity. We identify the auxin-dependent transcription factor ARF5/MONOPTEROS to cell-autonomously restrict the number of stem cells by directly attenuating the activity of the stem cell-promoting WOX4 gene. In contrast, ARF3 and ARF4 function as cambium activators in a redundant fashion from outside of WOX4-expressing cells. Our results reveal an influence of auxin signalling on distinct cambium features by specific signalling components and allow the conceptual integration of plant stem cell systems with distinct anatomies.
KW - Arabidopsis Proteins/biosynthesis
KW - Arabidopsis/genetics
KW - Cambium/cytology
KW - Cell Proliferation/physiology
KW - DNA-Binding Proteins/metabolism
KW - Gene Expression Profiling
KW - Gene Expression Regulation, Plant/genetics
KW - Homeodomain Proteins/biosynthesis
KW - Indoleacetic Acids/metabolism
KW - Nuclear Proteins/metabolism
KW - Plant Growth Regulators/metabolism
KW - Plants, Genetically Modified/metabolism
KW - Signal Transduction
KW - Stem Cells/cytology
KW - Transcription Factors/metabolism
KW - Wood/cytology
KW - ARABIDOPSIS-THALIANA
KW - SHOOT
KW - ROOT APICAL MERISTEM
KW - FACTOR MONOPTEROS
KW - VASCULAR CELL-DIVISION
KW - HYBRID ASPEN
KW - SECONDARY GROWTH
KW - GENE FAMILY
KW - PLANTS
KW - TRANSCRIPTION FACTOR
UR - http://www.scopus.com/inward/record.url?scp=85042747901&partnerID=8YFLogxK
U2 - 10.1038/s41467-018-03256-2
DO - 10.1038/s41467-018-03256-2
M3 - Article
C2 - 29491423
AN - SCOPUS:85042747901
VL - 9
SP - 875
JO - Nature Communications
JF - Nature Communications
SN - 2041-1723
IS - 1
M1 - 875
ER -
ID: 10425801