Spatial organization of fibroblast and spermatocyte nuclei with different B-chromosome content in Korean field mouse, Apodemus peninsulae (Rodentia, Muridae)

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Spatial organization of fibroblast and spermatocyte nuclei with different B-chromosome content in Korean field mouse, Apodemus peninsulae (Rodentia, Muridae). / Karamysheva, Tatyana V.; Torgasheva, Anna A.; Yefremov, Yaroslav R. et al.

In: Genome, Vol. 60, No. 10, 10.2017, p. 815-824.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{988998cb181a4a4c9ae9085df3d60fb1,

title = "Spatial organization of fibroblast and spermatocyte nuclei with different B-chromosome content in Korean field mouse, Apodemus peninsulae (Rodentia, Muridae)",

abstract = "Korean field mouse (Apodemus peninsulae) shows a wide variation in the number of B chromosomes composed of constitutive heterochromatin. For this reason, it provides a good model to study the influence of the number of centromeres and amount of heterochromatin on spatial organization of interphase nuclei. We analyzed the three-dimensional organization of fibroblast and spermatocyte nuclei of the field mice carrying a different number of B chromosomes using laser scanning microscopy and 3D fluorescence in situ hybridization. We detected a co-localization of the B chromosomes with constitutive heterochromatin of the chromosomes of the basic set. We showed a non-random distribution of B chromosomes in the spermatocyte nuclei. Unpaired B chromosomes showed a tendency to occur in the compartment formed by the unpaired part of the XY bivalent.",

keywords = "A chromosomes, B chromosomes, Centromeres, Confocal microscopy, FISH on three-dimensionally preserved nuclei, Spermatocytes, Three-dimensional architecture of the nucleus, CELLS, CHROMATIN, three-dimensional architecture of the nucleus, MAMMALIAN MEIOSIS, CENTROMERES, INTERPHASE, GENOME, spermatocytes, confocal microscopy, COMPARATIVE FISH ANALYSIS, ARCHITECTURE, centromeres, MICE, MICRODISSECTION",

author = "Karamysheva, {Tatyana V.} and Torgasheva, {Anna A.} and Yefremov, {Yaroslav R.} and Bogomolov, {Anton G.} and Thomas Liehr and Borodin, {Pavel M.} and Rubtsov, {Nikolay B.} and M. Puertas",

note = "Publisher Copyright: {\textcopyright} 2017 Published by NRC Research Press.",

year = "2017",

month = oct,

doi = "10.1139/gen-2017-0029",

language = "English",

volume = "60",

pages = "815--824",

journal = "Genome",

issn = "0831-2796",

publisher = "National Research Council of Canada",

number = "10",

}

RIS

TY - JOUR

T1 - Spatial organization of fibroblast and spermatocyte nuclei with different B-chromosome content in Korean field mouse, Apodemus peninsulae (Rodentia, Muridae)

AU - Karamysheva, Tatyana V.

AU - Torgasheva, Anna A.

AU - Yefremov, Yaroslav R.

AU - Bogomolov, Anton G.

AU - Liehr, Thomas

AU - Borodin, Pavel M.

AU - Rubtsov, Nikolay B.

AU - Puertas, M.

PY - 2017/10

Y1 - 2017/10

N2 - Korean field mouse (Apodemus peninsulae) shows a wide variation in the number of B chromosomes composed of constitutive heterochromatin. For this reason, it provides a good model to study the influence of the number of centromeres and amount of heterochromatin on spatial organization of interphase nuclei. We analyzed the three-dimensional organization of fibroblast and spermatocyte nuclei of the field mice carrying a different number of B chromosomes using laser scanning microscopy and 3D fluorescence in situ hybridization. We detected a co-localization of the B chromosomes with constitutive heterochromatin of the chromosomes of the basic set. We showed a non-random distribution of B chromosomes in the spermatocyte nuclei. Unpaired B chromosomes showed a tendency to occur in the compartment formed by the unpaired part of the XY bivalent.

AB - Korean field mouse (Apodemus peninsulae) shows a wide variation in the number of B chromosomes composed of constitutive heterochromatin. For this reason, it provides a good model to study the influence of the number of centromeres and amount of heterochromatin on spatial organization of interphase nuclei. We analyzed the three-dimensional organization of fibroblast and spermatocyte nuclei of the field mice carrying a different number of B chromosomes using laser scanning microscopy and 3D fluorescence in situ hybridization. We detected a co-localization of the B chromosomes with constitutive heterochromatin of the chromosomes of the basic set. We showed a non-random distribution of B chromosomes in the spermatocyte nuclei. Unpaired B chromosomes showed a tendency to occur in the compartment formed by the unpaired part of the XY bivalent.

KW - A chromosomes

KW - B chromosomes

KW - Centromeres

KW - Confocal microscopy

KW - FISH on three-dimensionally preserved nuclei

KW - Spermatocytes

KW - Three-dimensional architecture of the nucleus

KW - CELLS

KW - CHROMATIN

KW - three-dimensional architecture of the nucleus

KW - MAMMALIAN MEIOSIS

KW - CENTROMERES

KW - INTERPHASE

KW - GENOME

KW - spermatocytes

KW - confocal microscopy

KW - COMPARATIVE FISH ANALYSIS

KW - ARCHITECTURE

KW - centromeres

KW - MICE

KW - MICRODISSECTION

UR - http://www.scopus.com/inward/record.url?scp=85032965607&partnerID=8YFLogxK

U2 - 10.1139/gen-2017-0029

DO - 10.1139/gen-2017-0029

M3 - Article

C2 - 28732174

AN - SCOPUS:85032965607

VL - 60

SP - 815

EP - 824

JO - Genome

JF - Genome

SN - 0831-2796

IS - 10

ER -

ID: 8967368