Research output: Contribution to journal › Article › peer-review
Physiological, biochemical and genetic responses of Caucasian tea (Camellia sinensis (L.) Kuntze) genotypes under cold and frost stress. / Samarina, Lidiia S.; Malyukova, Lyudmila S.; Efremov, Alexander M. et al.
In: PeerJ, Vol. 8, e9787, 28.08.2020.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Physiological, biochemical and genetic responses of Caucasian tea (Camellia sinensis (L.) Kuntze) genotypes under cold and frost stress
AU - Samarina, Lidiia S.
AU - Malyukova, Lyudmila S.
AU - Efremov, Alexander M.
AU - Simonyan, Taisiya A.
AU - Matskiv, Alexandra O.
AU - Koninskaya, Natalia G.
AU - Rakhmangulov, Ruslan S.
AU - Gvasaliya, Maya V.
AU - Malyarovskaya, Valentina I.
AU - Ryndin, Alexey V.
AU - Orlov, Yuriy L.
AU - Tong, Wei
AU - Hanke, Magda Viola
N1 - Publisher Copyright: © 2020 Samarina et al. Distributed under Creative Commons CC-BY 4.0 Copyright: Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020/8/28
Y1 - 2020/8/28
N2 - Background. Cold and frost are two serious factors limiting the yield of many crops worldwide, including the tea plant (Camellia sinensis (L.) Kuntze). The acclimatization of tea plant from tropical to temperate climate regions resulted in unique germplasm in the North-Western Caucasus with extremely frost-tolerant genotypes. Methods. The aim of the current research was to evaluate the physiological, biochemical and genetic responses of tolerant and sensitive tea cultivars exposed to cold (0 to +2 ◦C for 7 days) and frost (−6 to −8 ◦C for 5 days). Relative water content, cell membranes integrity, pH of the cell sap, water soluble protein, cations, sugars, amino acids were measured under cold and frost. Comparative expression of the following genes ICE1, CBF1, WRKY2, DHN1, DHN2, DHN3, NAC17, NAC26, NAC30, SnRK1.1, SnRK1.2, SnRK1.3, bHLH7, bHLH43, P5CS, LOX1, LOX6, LOX7 were analyzed. Results. We found elevated protein (by 3-4 times) and cations (potassium, calcium and magnesium) contents in the leaves of both cultivars under cold and frost treatments. Meanwhile, Leu, Met, Val, Thr, Ser were increased under cold and frost, however tolerant cv. Gruzinskii7 showed earlier accumulation of these amino acids. Out of 18 studied genes, 11 were expressed at greater level in the frost- tolerant cultivar comparing with frost-sensitive one: ICE1, CBF1, WRKY2, DHN2, NAC17, NAC26, SnRK1.1, SnRK1.3, bHLH43, P5CS and LOX6. Positive correlations between certain amino acids namely, Met, Thr, Leu and Ser and studied genes were found. Taken together, the revealed cold responses in Caucasian tea cultivars help better understanding of tea tolerance to low temperature stress and role of revealed metabolites need to be further evaluated in different tea genotypes.
AB - Background. Cold and frost are two serious factors limiting the yield of many crops worldwide, including the tea plant (Camellia sinensis (L.) Kuntze). The acclimatization of tea plant from tropical to temperate climate regions resulted in unique germplasm in the North-Western Caucasus with extremely frost-tolerant genotypes. Methods. The aim of the current research was to evaluate the physiological, biochemical and genetic responses of tolerant and sensitive tea cultivars exposed to cold (0 to +2 ◦C for 7 days) and frost (−6 to −8 ◦C for 5 days). Relative water content, cell membranes integrity, pH of the cell sap, water soluble protein, cations, sugars, amino acids were measured under cold and frost. Comparative expression of the following genes ICE1, CBF1, WRKY2, DHN1, DHN2, DHN3, NAC17, NAC26, NAC30, SnRK1.1, SnRK1.2, SnRK1.3, bHLH7, bHLH43, P5CS, LOX1, LOX6, LOX7 were analyzed. Results. We found elevated protein (by 3-4 times) and cations (potassium, calcium and magnesium) contents in the leaves of both cultivars under cold and frost treatments. Meanwhile, Leu, Met, Val, Thr, Ser were increased under cold and frost, however tolerant cv. Gruzinskii7 showed earlier accumulation of these amino acids. Out of 18 studied genes, 11 were expressed at greater level in the frost- tolerant cultivar comparing with frost-sensitive one: ICE1, CBF1, WRKY2, DHN2, NAC17, NAC26, SnRK1.1, SnRK1.3, bHLH43, P5CS and LOX6. Positive correlations between certain amino acids namely, Met, Thr, Leu and Ser and studied genes were found. Taken together, the revealed cold responses in Caucasian tea cultivars help better understanding of tea tolerance to low temperature stress and role of revealed metabolites need to be further evaluated in different tea genotypes.
KW - Amino acids content
KW - Camellia sinensis
KW - Cations
KW - Climate adaptation
KW - Frost tolerance
KW - Gene expression
KW - Osmotic stress
KW - Plant physiology
KW - TRANSCRIPTION FACTORS
KW - PLANT
KW - TOLERANCE
KW - SUGAR
KW - ACCLIMATION
KW - ROLES
KW - EXPRESSION
KW - PCR
UR - http://www.scopus.com/inward/record.url?scp=85091478135&partnerID=8YFLogxK
U2 - 10.7717/peerj.9787
DO - 10.7717/peerj.9787
M3 - Article
C2 - 32923182
AN - SCOPUS:85091478135
VL - 8
JO - PeerJ
JF - PeerJ
SN - 2167-8359
M1 - e9787
ER -
ID: 25627026