Photoinduced inhibition of DNA repair enzymes and the possible mechanism of photochemical transformations of the ruthenium nitrosyl complex [RuNO(β-Pic)2(NO2)2OH

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Photoinduced inhibition of DNA repair enzymes and the possible mechanism of photochemical transformations of the ruthenium nitrosyl complex [RuNO(β-Pic)₂(NO₂)₂OH. / Mikhailov, Artem A.; Khantakova, Darya V.; Nichiporenko, Vladislav A. et al.

In: Metallomics : integrated biometal science, Vol. 11, No. 12, 11.12.2019, p. 1999-2009.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{8352bb2483824a06982e73164c60737d,

title = "Photoinduced inhibition of DNA repair enzymes and the possible mechanism of photochemical transformations of the ruthenium nitrosyl complex [RuNO(β-Pic)2(NO2)2OH",

abstract = "In this work we have demonstrated that the ruthenium nitrosyl complex [RuNO(β-Pic)2(NO2)2OH] is suitable for investigation of the inactivation of DNA repair enzymes in vitro. Photoinduced inhibition of DNA glycosylases such as E. coli Endo III, plant NtROS1, mammalian mNEIL1 and hNEIL2 occurs to an extent of ≥90% after irradiation with the ruthenium complex. The photophysical and photochemical processes of [RuNO(β-Pic)2(NO2)2OH] were investigated using stationary and time-resolved spectroscopy, and mass spectrometry. A possible mechanism of the photo-processes was proposed from the combined spectroscopic study and DTF calculations, which reveal that the photolysis is multistage. The primary and secondary photolysis stages are the photo-induced cleavage of the Ru-NO bond with the formation of a free nitric oxide and RuIII complex followed by ligand exchange with solvent. For E. coli Endo III, covalent interaction with the photolysis product was confirmed by UV-vis and mass spectrometric methods.",

keywords = "COLI ENDONUCLEASE-III, NITRIC-OXIDE, ESCHERICHIA-COLI, BIOLOGICAL-PROPERTIES, PHOTODYNAMIC THERAPY, NO, LIGANDS, ACID, PHOTORELEASE, SPECIFICITY",

author = "Mikhailov, {Artem A.} and Khantakova, {Darya V.} and Nichiporenko, {Vladislav A.} and Glebov, {Evgeni M.} and Grivin, {Vjacheslav P.} and Plyusnin, {Victor F.} and Yanshole, {Vadim V.} and Petrova, {Daria V.} and Kostin, {Gennadiy A.} and Grin, {Inga R.}",

year = "2019",

month = dec,

day = "11",

doi = "10.1039/c9mt00153k",

language = "English",

volume = "11",

pages = "1999--2009",

journal = "Metallomics : integrated biometal science",

issn = "1756-5901",

publisher = "Oxford University Press",

number = "12",

}

RIS

TY - JOUR

T1 - Photoinduced inhibition of DNA repair enzymes and the possible mechanism of photochemical transformations of the ruthenium nitrosyl complex [RuNO(β-Pic)2(NO2)2OH

AU - Mikhailov, Artem A.

AU - Khantakova, Darya V.

AU - Nichiporenko, Vladislav A.

AU - Glebov, Evgeni M.

AU - Grivin, Vjacheslav P.

AU - Plyusnin, Victor F.

AU - Yanshole, Vadim V.

AU - Petrova, Daria V.

AU - Kostin, Gennadiy A.

AU - Grin, Inga R.

PY - 2019/12/11

Y1 - 2019/12/11

N2 - In this work we have demonstrated that the ruthenium nitrosyl complex [RuNO(β-Pic)2(NO2)2OH] is suitable for investigation of the inactivation of DNA repair enzymes in vitro. Photoinduced inhibition of DNA glycosylases such as E. coli Endo III, plant NtROS1, mammalian mNEIL1 and hNEIL2 occurs to an extent of ≥90% after irradiation with the ruthenium complex. The photophysical and photochemical processes of [RuNO(β-Pic)2(NO2)2OH] were investigated using stationary and time-resolved spectroscopy, and mass spectrometry. A possible mechanism of the photo-processes was proposed from the combined spectroscopic study and DTF calculations, which reveal that the photolysis is multistage. The primary and secondary photolysis stages are the photo-induced cleavage of the Ru-NO bond with the formation of a free nitric oxide and RuIII complex followed by ligand exchange with solvent. For E. coli Endo III, covalent interaction with the photolysis product was confirmed by UV-vis and mass spectrometric methods.

AB - In this work we have demonstrated that the ruthenium nitrosyl complex [RuNO(β-Pic)2(NO2)2OH] is suitable for investigation of the inactivation of DNA repair enzymes in vitro. Photoinduced inhibition of DNA glycosylases such as E. coli Endo III, plant NtROS1, mammalian mNEIL1 and hNEIL2 occurs to an extent of ≥90% after irradiation with the ruthenium complex. The photophysical and photochemical processes of [RuNO(β-Pic)2(NO2)2OH] were investigated using stationary and time-resolved spectroscopy, and mass spectrometry. A possible mechanism of the photo-processes was proposed from the combined spectroscopic study and DTF calculations, which reveal that the photolysis is multistage. The primary and secondary photolysis stages are the photo-induced cleavage of the Ru-NO bond with the formation of a free nitric oxide and RuIII complex followed by ligand exchange with solvent. For E. coli Endo III, covalent interaction with the photolysis product was confirmed by UV-vis and mass spectrometric methods.

KW - COLI ENDONUCLEASE-III

KW - NITRIC-OXIDE

KW - ESCHERICHIA-COLI

KW - BIOLOGICAL-PROPERTIES

KW - PHOTODYNAMIC THERAPY

KW - NO

KW - LIGANDS

KW - ACID

KW - PHOTORELEASE

KW - SPECIFICITY

UR - http://www.scopus.com/inward/record.url?scp=85076382709&partnerID=8YFLogxK

U2 - 10.1039/c9mt00153k

DO - 10.1039/c9mt00153k

M3 - Article

C2 - 31555793

AN - SCOPUS:85076382709

VL - 11

SP - 1999

EP - 2009

JO - Metallomics : integrated biometal science

JF - Metallomics : integrated biometal science

SN - 1756-5901

IS - 12

ER -

ID: 22665544