Research output: Contribution to journal › Article › peer-review
Pathology of A(H5N8) (Clade 2.3.4.4) Virus in Experimentally Infected Chickens and Mice. / Prokopyeva, Elena A.; Zinserling, Vsevolod A.; Bae, You Chan et al.
In: Interdisciplinary Perspectives on Infectious Diseases, Vol. 2019, 4124865, 01.01.2019, p. 4124865.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Pathology of A(H5N8) (Clade 2.3.4.4) Virus in Experimentally Infected Chickens and Mice
AU - Prokopyeva, Elena A.
AU - Zinserling, Vsevolod A.
AU - Bae, You Chan
AU - Kwon, Yongkuk
AU - Kurskaya, Olga G.
AU - Sobolev, Ivan A.
AU - Kozhin, Peter M.
AU - Komissarov, Andrey
AU - Fadeev, Artem
AU - Petrov, Vladimir
AU - Shestopalov, Alexander M.
AU - Sharshov, Kirill A.
PY - 2019/1/1
Y1 - 2019/1/1
N2 - The emergence of novel highly pathogenic avian influenza viruses (HPAIVs) in migratory birds raises serious concerns as these viruses have the potential to spread during fall migration. We report the identification of novel HPAIV A(H5N8) clade 2.3.4.4 virus that was isolated from sick domestic duck at commercial farm during the second wave of spread that began in October and affected poultry (ducks; chickens) in several European regions of Russia and Western Siberia in 2016. The strain was highly lethal in experimental infection of chickens and mice with IVPI = 2.34 and MLD50 = 1.3log10 = EID50, accordingly. Inoculation of chickens with the HPAIV A/H5N8 demonstrated neuroinvasiveness, multiorgan failure, and death of chickens on the 3rd day post inoculation. Virus replicated in all collected organ samples in high viral titers with the highest titer in the brain (6.75±0.1 log10 TCID50/ml). Effective virus replication was found in the following cells: neurons and glial cells of a brain; alveolar cells and macrophages of lungs; epithelial cells of a small intestine; hepatocytes and Kupffer cells of a liver; macrophages and endothelial cells of a spleen; and the tubular epithelial cells of kidneys. These findings advance our understanding of histopathological effect of A(H5N8) HPAIV infection.
AB - The emergence of novel highly pathogenic avian influenza viruses (HPAIVs) in migratory birds raises serious concerns as these viruses have the potential to spread during fall migration. We report the identification of novel HPAIV A(H5N8) clade 2.3.4.4 virus that was isolated from sick domestic duck at commercial farm during the second wave of spread that began in October and affected poultry (ducks; chickens) in several European regions of Russia and Western Siberia in 2016. The strain was highly lethal in experimental infection of chickens and mice with IVPI = 2.34 and MLD50 = 1.3log10 = EID50, accordingly. Inoculation of chickens with the HPAIV A/H5N8 demonstrated neuroinvasiveness, multiorgan failure, and death of chickens on the 3rd day post inoculation. Virus replicated in all collected organ samples in high viral titers with the highest titer in the brain (6.75±0.1 log10 TCID50/ml). Effective virus replication was found in the following cells: neurons and glial cells of a brain; alveolar cells and macrophages of lungs; epithelial cells of a small intestine; hepatocytes and Kupffer cells of a liver; macrophages and endothelial cells of a spleen; and the tubular epithelial cells of kidneys. These findings advance our understanding of histopathological effect of A(H5N8) HPAIV infection.
UR - http://www.scopus.com/inward/record.url?scp=85069044516&partnerID=8YFLogxK
U2 - 10.1155/2019/4124865
DO - 10.1155/2019/4124865
M3 - Article
C2 - 31354812
AN - SCOPUS:85069044516
VL - 2019
SP - 4124865
JO - Interdisciplinary Perspectives on Infectious Diseases
JF - Interdisciplinary Perspectives on Infectious Diseases
SN - 1687-708X
M1 - 4124865
ER -
ID: 20885935