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New insights from Opisthorchis felineus genome : update on genomics of the epidemiologically important liver flukes. / Ershov, Nikita I.; Mordvinov, Viatcheslav A.; Prokhortchouk, Egor B. et al.
In: BMC Genomics, Vol. 20, No. 1, 399, 22.05.2019, p. 399.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - New insights from Opisthorchis felineus genome
T2 - update on genomics of the epidemiologically important liver flukes
AU - Ershov, Nikita I.
AU - Mordvinov, Viatcheslav A.
AU - Prokhortchouk, Egor B.
AU - Pakharukova, Mariya Y.
AU - Gunbin, Konstantin V.
AU - Ustyantsev, Kirill
AU - Genaev, Mikhail A.
AU - Blinov, Alexander G.
AU - Mazur, Alexander
AU - Boulygina, Eugenia
AU - Tsygankova, Svetlana
AU - Khrameeva, Ekaterina
AU - Chekanov, Nikolay
AU - Fan, Guangyi
AU - Xiao, An
AU - Zhang, He
AU - Xu, Xun
AU - Yang, Huanming
AU - Solovyev, Victor
AU - Lee, Simon Ming Yuen
AU - Liu, Xin
AU - Afonnikov, Dmitry A.
AU - Skryabin, Konstantin G.
PY - 2019/5/22
Y1 - 2019/5/22
N2 - BACKGROUND: The three epidemiologically important Opisthorchiidae liver flukes Opisthorchis felineus, O. viverrini, and Clonorchis sinensis, are believed to harbour similar potencies to provoke hepatobiliary diseases in their definitive hosts, although their populations have substantially different ecogeographical aspects including habitat, preferred hosts, population structure. Lack of O. felineus genomic data is an obstacle to the development of comparative molecular biological approaches necessary to obtain new knowledge about the biology of Opisthorchiidae trematodes, to identify essential pathways linked to parasite-host interaction, to predict genes that contribute to liver fluke pathogenesis and for the effective prevention and control of the disease. RESULTS: Here we present the first draft genome assembly of O. felineus and its gene repertoire accompanied by a comparative analysis with that of O. viverrini and Clonorchis sinensis. We observed both noticeably high heterozygosity of the sequenced individual and substantial genetic diversity in a pooled sample. This indicates that potency of O. felineus population for rapid adaptive response to control and preventive measures of opisthorchiasis is higher than in O. viverrini and C. sinensis. We also have found that all three species are characterized by more intensive involvement of trans-splicing in RNA processing compared to other trematodes. CONCLUSION: All revealed peculiarities of structural organization of genomes are of extreme importance for a proper description of genes and their products in these parasitic species. This should be taken into account both in academic and applied research of epidemiologically important liver flukes. Further comparative genomics studies of liver flukes and non-carcinogenic flatworms allow for generation of well-grounded hypotheses on the mechanisms underlying development of cholangiocarcinoma associated with opisthorchiasis and clonorchiasis as well as species-specific mechanisms of these diseases.
AB - BACKGROUND: The three epidemiologically important Opisthorchiidae liver flukes Opisthorchis felineus, O. viverrini, and Clonorchis sinensis, are believed to harbour similar potencies to provoke hepatobiliary diseases in their definitive hosts, although their populations have substantially different ecogeographical aspects including habitat, preferred hosts, population structure. Lack of O. felineus genomic data is an obstacle to the development of comparative molecular biological approaches necessary to obtain new knowledge about the biology of Opisthorchiidae trematodes, to identify essential pathways linked to parasite-host interaction, to predict genes that contribute to liver fluke pathogenesis and for the effective prevention and control of the disease. RESULTS: Here we present the first draft genome assembly of O. felineus and its gene repertoire accompanied by a comparative analysis with that of O. viverrini and Clonorchis sinensis. We observed both noticeably high heterozygosity of the sequenced individual and substantial genetic diversity in a pooled sample. This indicates that potency of O. felineus population for rapid adaptive response to control and preventive measures of opisthorchiasis is higher than in O. viverrini and C. sinensis. We also have found that all three species are characterized by more intensive involvement of trans-splicing in RNA processing compared to other trematodes. CONCLUSION: All revealed peculiarities of structural organization of genomes are of extreme importance for a proper description of genes and their products in these parasitic species. This should be taken into account both in academic and applied research of epidemiologically important liver flukes. Further comparative genomics studies of liver flukes and non-carcinogenic flatworms allow for generation of well-grounded hypotheses on the mechanisms underlying development of cholangiocarcinoma associated with opisthorchiasis and clonorchiasis as well as species-specific mechanisms of these diseases.
KW - Genome
KW - Liver flukes
KW - Metacercariae
KW - Microintrons
KW - Opisthorchiidae
KW - Opisthorchis felineus
KW - Trans-splicing
KW - Transcriptome
KW - SPECIES TREMATODA
KW - PHYLOGENETIC ANALYSIS
KW - CLONORCHIS-SINENSIS
KW - MITOCHONDRIAL GENOMES
KW - VIVERRINI
KW - IN-VITRO
KW - ANNOTATION
KW - SEQUENCE
KW - GENES
KW - INFECTION
UR - http://www.scopus.com/inward/record.url?scp=85066508291&partnerID=8YFLogxK
U2 - 10.1186/s12864-019-5752-8
DO - 10.1186/s12864-019-5752-8
M3 - Article
C2 - 31117933
AN - SCOPUS:85066508291
VL - 20
SP - 399
JO - BMC Genomics
JF - BMC Genomics
SN - 1471-2164
IS - 1
M1 - 399
ER -
ID: 20344873