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Membrane-Sugar Interactions Probed by Pulsed Electron Paramagnetic Resonance of Spin Labels. / Konov, Konstantin B.; Leonov, Dmitry V.; Isaev, Nikolay P. et al.

In: Journal of Physical Chemistry B, Vol. 119, No. 32, 13.08.2015, p. 10261-10266.

Research output: Contribution to journal › Article › peer-review

Harvard

Konov, KB, Leonov, DV, Isaev, NP, Fedotov, KY, Voronkova, VK & Dzuba, SA 2015, 'Membrane-Sugar Interactions Probed by Pulsed Electron Paramagnetic Resonance of Spin Labels', Journal of Physical Chemistry B, vol. 119, no. 32, pp. 10261-10266. https://doi.org/10.1021/acs.jpcb.5b06864

APA

Konov, K. B., Leonov, D. V., Isaev, N. P., Fedotov, K. Y., Voronkova, V. K., & Dzuba, S. A. (2015). Membrane-Sugar Interactions Probed by Pulsed Electron Paramagnetic Resonance of Spin Labels. Journal of Physical Chemistry B, 119(32), 10261-10266. https://doi.org/10.1021/acs.jpcb.5b06864

Vancouver

Konov KB, Leonov DV, Isaev NP, Fedotov KY, Voronkova VK, Dzuba SA. Membrane-Sugar Interactions Probed by Pulsed Electron Paramagnetic Resonance of Spin Labels. Journal of Physical Chemistry B. 2015 Aug 13;119(32):10261-10266. doi: 10.1021/acs.jpcb.5b06864

Author

Konov, Konstantin B. ; Leonov, Dmitry V. ; Isaev, Nikolay P. et al. / Membrane-Sugar Interactions Probed by Pulsed Electron Paramagnetic Resonance of Spin Labels. In: Journal of Physical Chemistry B. 2015 ; Vol. 119, No. 32. pp. 10261-10266.

BibTeX

@article{0ea64197c63e4cb6a912b9e6647514a8,
title = "Membrane-Sugar Interactions Probed by Pulsed Electron Paramagnetic Resonance of Spin Labels",
abstract = "Sugars can stabilize biological systems under extreme desiccation and freezing conditions. Hypothetical molecular mechanisms suggest that the stabilization effect may be determined either by specific interactions of sugars with biological molecules or by the influence of sugars on the solvating shell of the biomolecule. To explore membrane-sugar interactions, we applied electron spin echo envelope modulation (ESEEM) spectroscopy, a pulsed version of electron paramagnetic resonance (EPR), to phospholipid bilayers with spin-labeled lipids added and solvated by aqueous deuterated sucrose and trehalose solutions. The phospholipids were 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC). The spin-labeled lipids were 1,2-dipalmitoyl-sn-glycero-3-phospho(TEMPO)choline (T-PCSL), with spin-label TEMPO at the lipid polar headgroup. The deuterium ESEEM amplitude was calibrated using known concentrations of glassy deuterated sugar solvents. The data obtained indicated that the sugar concentration near the membrane surface obeyed a simple Langmuir model of monolayer adsorption, which assumes direct sugar-molecule bonding to the bilayer surface.",
author = "Konov, {Konstantin B.} and Leonov, {Dmitry V.} and Isaev, {Nikolay P.} and Fedotov, {Kirill Yu} and Voronkova, {Violeta K.} and Dzuba, {Sergei A.}",
year = "2015",
month = aug,
day = "13",
doi = "10.1021/acs.jpcb.5b06864",
language = "English",
volume = "119",
pages = "10261--10266",
journal = "Journal of Physical Chemistry B",
issn = "1520-6106",
publisher = "American Chemical Society",
number = "32",

}

RIS

TY - JOUR

T1 - Membrane-Sugar Interactions Probed by Pulsed Electron Paramagnetic Resonance of Spin Labels

AU - Konov, Konstantin B.

AU - Leonov, Dmitry V.

AU - Isaev, Nikolay P.

AU - Fedotov, Kirill Yu

AU - Voronkova, Violeta K.

AU - Dzuba, Sergei A.

PY - 2015/8/13

Y1 - 2015/8/13

N2 - Sugars can stabilize biological systems under extreme desiccation and freezing conditions. Hypothetical molecular mechanisms suggest that the stabilization effect may be determined either by specific interactions of sugars with biological molecules or by the influence of sugars on the solvating shell of the biomolecule. To explore membrane-sugar interactions, we applied electron spin echo envelope modulation (ESEEM) spectroscopy, a pulsed version of electron paramagnetic resonance (EPR), to phospholipid bilayers with spin-labeled lipids added and solvated by aqueous deuterated sucrose and trehalose solutions. The phospholipids were 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC). The spin-labeled lipids were 1,2-dipalmitoyl-sn-glycero-3-phospho(TEMPO)choline (T-PCSL), with spin-label TEMPO at the lipid polar headgroup. The deuterium ESEEM amplitude was calibrated using known concentrations of glassy deuterated sugar solvents. The data obtained indicated that the sugar concentration near the membrane surface obeyed a simple Langmuir model of monolayer adsorption, which assumes direct sugar-molecule bonding to the bilayer surface.

AB - Sugars can stabilize biological systems under extreme desiccation and freezing conditions. Hypothetical molecular mechanisms suggest that the stabilization effect may be determined either by specific interactions of sugars with biological molecules or by the influence of sugars on the solvating shell of the biomolecule. To explore membrane-sugar interactions, we applied electron spin echo envelope modulation (ESEEM) spectroscopy, a pulsed version of electron paramagnetic resonance (EPR), to phospholipid bilayers with spin-labeled lipids added and solvated by aqueous deuterated sucrose and trehalose solutions. The phospholipids were 1,2-dipalmitoyl-sn-glycero-3-phosphocholine (DPPC). The spin-labeled lipids were 1,2-dipalmitoyl-sn-glycero-3-phospho(TEMPO)choline (T-PCSL), with spin-label TEMPO at the lipid polar headgroup. The deuterium ESEEM amplitude was calibrated using known concentrations of glassy deuterated sugar solvents. The data obtained indicated that the sugar concentration near the membrane surface obeyed a simple Langmuir model of monolayer adsorption, which assumes direct sugar-molecule bonding to the bilayer surface.

UR - http://www.scopus.com/inward/record.url?scp=84939245868&partnerID=8YFLogxK

U2 - 10.1021/acs.jpcb.5b06864

DO - 10.1021/acs.jpcb.5b06864

M3 - Article

AN - SCOPUS:84939245868

VL - 119

SP - 10261

EP - 10266

JO - Journal of Physical Chemistry B

JF - Journal of Physical Chemistry B

SN - 1520-6106

IS - 32

ER -

ID: 25832048