TY - JOUR

T1 - Interbreed variation in meiotic recombination rate and distribution in the domestic chicken Gallus gallus

AU - Malinovskaya, Lyubov P.

AU - Tishakova, Katerina V.

AU - Volkova, Natalia A.

AU - Torgasheva, Anna A.

AU - Tsepilov, Yakov A.

AU - Borodin, Pavel M.

N1 - Copyright: © 2019 Lyubov P. Malinovskaya et al.

PY - 2019/7/10

Y1 - 2019/7/10

N2 - The efficiency of natural and artificial selection is critically dependent on the recombination rate. However, interbreed and individual variation in recombination rate in poultry remains unknown. Conventional methods of analysis of recombination such as genetic linkage analysis, sperm genotyping and chiasma count at lampbrush chromosomes are expensive and time-consuming. In this study, we analyzed the number and distribution of recombination nodules in spermatocytes of the roosters of six chicken breeds using immunolocalization of key proteins involved in chromosome pairing and recombination. We revealed significant effects of breed (R2 = 0:17; p < 0:001) and individual (R2 = 0:28; p < 0:001) on variation in the number of recombination nodules. Both interbreed and individual variations in recombination rate were almost entirely determined by variation in recombination density on macrochromosomes, because almost all microchromosomes in each breed had one recombination nodule. Despite interbreed differences in the density of recombination nodules, the patterns of their distribution along homologous chromosomes were similar. The breeds examined in this study showed a correspondence between the age of the breed and its recombination rate. Those with high recombination rates (Pervomai, Russian White and Brahma) are relatively young breeds created by crossing several local breeds. The breeds displaying low recombination rate are ancient local breeds: Cochin (Indo-China), Brown Leghorn (Tuscany, Italy) and Russian Crested (the European part of Russia).

AB - The efficiency of natural and artificial selection is critically dependent on the recombination rate. However, interbreed and individual variation in recombination rate in poultry remains unknown. Conventional methods of analysis of recombination such as genetic linkage analysis, sperm genotyping and chiasma count at lampbrush chromosomes are expensive and time-consuming. In this study, we analyzed the number and distribution of recombination nodules in spermatocytes of the roosters of six chicken breeds using immunolocalization of key proteins involved in chromosome pairing and recombination. We revealed significant effects of breed (R2 = 0:17; p < 0:001) and individual (R2 = 0:28; p < 0:001) on variation in the number of recombination nodules. Both interbreed and individual variations in recombination rate were almost entirely determined by variation in recombination density on macrochromosomes, because almost all microchromosomes in each breed had one recombination nodule. Despite interbreed differences in the density of recombination nodules, the patterns of their distribution along homologous chromosomes were similar. The breeds examined in this study showed a correspondence between the age of the breed and its recombination rate. Those with high recombination rates (Pervomai, Russian White and Brahma) are relatively young breeds created by crossing several local breeds. The breeds displaying low recombination rate are ancient local breeds: Cochin (Indo-China), Brown Leghorn (Tuscany, Italy) and Russian Crested (the European part of Russia).

KW - SYNAPTONEMAL COMPLEXES

KW - SELECTION

KW - MOUSE

KW - EVOLUTION

KW - GENOME

KW - CHROMOSOMES

KW - FREQUENCIES

KW - LENGTH

KW - BIRDS

KW - MAPS

UR - http://www.scopus.com/inward/record.url?scp=85069055913&partnerID=8YFLogxK

U2 - 10.5194/aab-62-403-2019

DO - 10.5194/aab-62-403-2019

M3 - Article

C2 - 31807651

AN - SCOPUS:85069055913

VL - 62

SP - 403

EP - 411

JO - Archives Animal Breeding

JF - Archives Animal Breeding

SN - 0003-9438

IS - 2

ER -