Research output: Contribution to journal › Review article › peer-review
Human placenta exosomes : Biogenesis, isolation, composition, and prospects for use in diagnostics. / Burkova, Evgeniya E.; Sedykh, Sergey E.; Nevinsky, Georgy A.
In: International Journal of Molecular Sciences, Vol. 22, No. 4, 2158, 02.02.2021, p. 1-20.Research output: Contribution to journal › Review article › peer-review
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TY - JOUR
T1 - Human placenta exosomes
T2 - Biogenesis, isolation, composition, and prospects for use in diagnostics
AU - Burkova, Evgeniya E.
AU - Sedykh, Sergey E.
AU - Nevinsky, Georgy A.
N1 - Funding Information: Funding: This investigation was sustained by the project of RSF, number 18-74-10055 (to Sergey Sedykh), and the Russian state-funded budget project of ICBFM SB RAS # 0245-2021-0009 (to Georgy Nevinsky). Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland. Copyright: Copyright 2021 Elsevier B.V., All rights reserved.
PY - 2021/2/2
Y1 - 2021/2/2
N2 - Exosomes are 40–100 nm nanovesicles participating in intercellular communication and transferring various bioactive proteins, mRNAs, miRNAs, and lipids. During pregnancy, the placenta releases exosomes into the maternal circulation. Placental exosomes are detected in the maternal blood even in the first trimester of pregnancy and their numbers increase significantly by the end of pregnancy. Exosomes are necessary for the normal functioning of the placenta and fetal development. Effects of exosomes on target cells depend not only on their concentration but also on their intrinsic components. The biochemical composition of the placental exosomes may cause various complications of pregnancy. Some studies relate the changes in the composition of nanovesicles to placental dysfunction. Isolation of placental exosomes from the blood of pregnant women and the study of protein, lipid, and nucleic composition can lead to the development of methods for early diagnosis of pregnancy pathologies. This review describes the biogenesis of exosomes, methods of their isolation, analyzes their biochemical composition, and considers the prospects for using exosomes to diagnose pregnancy pathologies.
AB - Exosomes are 40–100 nm nanovesicles participating in intercellular communication and transferring various bioactive proteins, mRNAs, miRNAs, and lipids. During pregnancy, the placenta releases exosomes into the maternal circulation. Placental exosomes are detected in the maternal blood even in the first trimester of pregnancy and their numbers increase significantly by the end of pregnancy. Exosomes are necessary for the normal functioning of the placenta and fetal development. Effects of exosomes on target cells depend not only on their concentration but also on their intrinsic components. The biochemical composition of the placental exosomes may cause various complications of pregnancy. Some studies relate the changes in the composition of nanovesicles to placental dysfunction. Isolation of placental exosomes from the blood of pregnant women and the study of protein, lipid, and nucleic composition can lead to the development of methods for early diagnosis of pregnancy pathologies. This review describes the biogenesis of exosomes, methods of their isolation, analyzes their biochemical composition, and considers the prospects for using exosomes to diagnose pregnancy pathologies.
KW - Biomark-ers
KW - Exosomes
KW - Isolation
KW - Lipid
KW - MiRNA
KW - Placenta
KW - Placental exosomes
KW - Protein
KW - Trophoblast
UR - http://www.scopus.com/inward/record.url?scp=85100951221&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/11822603-528b-37b2-a113-d00ea8b46fcf/
U2 - 10.3390/ijms22042158
DO - 10.3390/ijms22042158
M3 - Review article
C2 - 33671527
AN - SCOPUS:85100951221
VL - 22
SP - 1
EP - 20
JO - International Journal of Molecular Sciences
JF - International Journal of Molecular Sciences
SN - 1661-6596
IS - 4
M1 - 2158
ER -
ID: 27963254