Research output: Contribution to journal › Article › peer-review
Highly conservative pattern of sex chromosome synapsis and recombination in neognathae birds. / Torgasheva, Anna; Malinovskaya, Lyubov; Zadesenets, Kira S. et al.
In: Genes, Vol. 12, No. 9, 1358, 09.2021.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Highly conservative pattern of sex chromosome synapsis and recombination in neognathae birds
AU - Torgasheva, Anna
AU - Malinovskaya, Lyubov
AU - Zadesenets, Kira S.
AU - Slobodchikova, Anastasia
AU - Shnaider, Elena
AU - Rubtsov, Nikolai
AU - Borodin, Pavel
N1 - Funding Information: Funding: The work was funded by Russian Science Foundation, grant number 20-64-46021. Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/9
Y1 - 2021/9
N2 - We analyzed the synapsis and recombination between Z and W chromosomes in the oocytes of nine neognath species: domestic chicken Gallus gallus domesticus, grey goose Anser anser, black tern Chlidonias niger, common tern Sterna hirundo, pale martin Riparia diluta, barn swallow Hirundo rustica, European pied flycatcher Ficedula hypoleuca, great tit Parus major and white wagtail Motacilla alba using immunolocalization of SYCP3, the main protein of the lateral elements of the synaptonemal complex, and MLH1, the mismatch repair protein marking mature recombination nodules. In all species examined, homologous synapsis occurs in a short region of variable size at the ends of Z and W chromosomes, where a single recombination nodule is located. The remaining parts of the sex chromosomes undergo synaptic adjustment and synapse non-homologously. In 25% of ZW bivalents of white wagtail, synapsis and recombination also occur at the secondary pairing region, which probably resulted from autosome−sex chromosome translocation. Using FISH with a paint probe specific to the germline-restricted chromosome (GRC) of the pale martin on the oocytes of the pale martin, barn swallow and great tit, we showed that both maternally inherited songbird chromosomes (GRC and W) share common sequences.
AB - We analyzed the synapsis and recombination between Z and W chromosomes in the oocytes of nine neognath species: domestic chicken Gallus gallus domesticus, grey goose Anser anser, black tern Chlidonias niger, common tern Sterna hirundo, pale martin Riparia diluta, barn swallow Hirundo rustica, European pied flycatcher Ficedula hypoleuca, great tit Parus major and white wagtail Motacilla alba using immunolocalization of SYCP3, the main protein of the lateral elements of the synaptonemal complex, and MLH1, the mismatch repair protein marking mature recombination nodules. In all species examined, homologous synapsis occurs in a short region of variable size at the ends of Z and W chromosomes, where a single recombination nodule is located. The remaining parts of the sex chromosomes undergo synaptic adjustment and synapse non-homologously. In 25% of ZW bivalents of white wagtail, synapsis and recombination also occur at the secondary pairing region, which probably resulted from autosome−sex chromosome translocation. Using FISH with a paint probe specific to the germline-restricted chromosome (GRC) of the pale martin on the oocytes of the pale martin, barn swallow and great tit, we showed that both maternally inherited songbird chromosomes (GRC and W) share common sequences.
KW - Avian sex chromosomes
KW - Crossing over
KW - MLH1
KW - Recombination nodules
KW - SYCP3
KW - Synaptonemal complex
UR - http://www.scopus.com/inward/record.url?scp=85114357556&partnerID=8YFLogxK
U2 - 10.3390/genes12091358
DO - 10.3390/genes12091358
M3 - Article
C2 - 34573341
AN - SCOPUS:85114357556
VL - 12
JO - Genes
JF - Genes
SN - 2073-4425
IS - 9
M1 - 1358
ER -
ID: 34161092