Research output: Contribution to journal › Article › peer-review
Heteroleptic Ruthenium(II) complexes with bathophenanthroline and bathophenanthroline disulfonate disodium salt as fluorescent dyes for In-Gel protein staining. / Babak, Maria V.; Le Faouder, Pauline; Trivelli, Xavier et al.
In: Inorganic Chemistry, Vol. 59, No. 7, 17.03.2020, p. 4527-4535.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Heteroleptic Ruthenium(II) complexes with bathophenanthroline and bathophenanthroline disulfonate disodium salt as fluorescent dyes for In-Gel protein staining
AU - Babak, Maria V.
AU - Le Faouder, Pauline
AU - Trivelli, Xavier
AU - Venkatesan, Gopalakrishnan
AU - Bezzubov, Stanislav I.
AU - Kajjout, Mohammed
AU - Gushchin, Artem L.
AU - Hanif, Muhammad
AU - Poizat, Olivier
AU - Vezin, Hervé
AU - Rolando, Christian
PY - 2020/3/17
Y1 - 2020/3/17
N2 - The in-gel detection of proteins for various proteomic experiments is commonly done with the fluorescent RuII tris(bathophenanthroline disulfonate) complex (Ru(BPS)3), which is more cost-effective compared to commercial Ru-based formulations but requires tedious procedures for its preparation and strongly acidic staining conditions. Herein, we report the synthesis and characterization of heteroleptic RuII complexes Ru(BPS)2(BP) and Ru(BPS)(BP)2 containing bathophenanthroline (BP) and bathophenanthroline disulfonate disodium salt (BPS) in comparison with Ru(BPS)3. It was shown by fluorescent and UV-vis measurements that novel RuII complexes were excitable in both UV and visible light, close to emission bands of classical lasers, which is important for successful in-gel protein detection. Novel fluorescent dyes demonstrated improved protein detection in comparison with commercially available SYPRO Ruby staining solution. In addition, unlike commonly used staining protocols, staining with Ru(BPS)(BP)2 can be performed at nearly neutral pH, thereby reducing artificial post-translational modifications (PTMs).
AB - The in-gel detection of proteins for various proteomic experiments is commonly done with the fluorescent RuII tris(bathophenanthroline disulfonate) complex (Ru(BPS)3), which is more cost-effective compared to commercial Ru-based formulations but requires tedious procedures for its preparation and strongly acidic staining conditions. Herein, we report the synthesis and characterization of heteroleptic RuII complexes Ru(BPS)2(BP) and Ru(BPS)(BP)2 containing bathophenanthroline (BP) and bathophenanthroline disulfonate disodium salt (BPS) in comparison with Ru(BPS)3. It was shown by fluorescent and UV-vis measurements that novel RuII complexes were excitable in both UV and visible light, close to emission bands of classical lasers, which is important for successful in-gel protein detection. Novel fluorescent dyes demonstrated improved protein detection in comparison with commercially available SYPRO Ruby staining solution. In addition, unlike commonly used staining protocols, staining with Ru(BPS)(BP)2 can be performed at nearly neutral pH, thereby reducing artificial post-translational modifications (PTMs).
KW - POLYACRYLAMIDE GELS
KW - SYPRO-RUBY
KW - ELECTROCHEMILUMINESCENCE
KW - ELECTROPHORESIS
KW - PHOTOPHYSICS
KW - ROUTE
KW - WATER
KW - ACIDS
UR - http://www.scopus.com/inward/record.url?scp=85082314450&partnerID=8YFLogxK
U2 - 10.1021/acs.inorgchem.9b03679
DO - 10.1021/acs.inorgchem.9b03679
M3 - Article
C2 - 32181663
AN - SCOPUS:85082314450
VL - 59
SP - 4527
EP - 4535
JO - Inorganic Chemistry
JF - Inorganic Chemistry
SN - 0020-1669
IS - 7
ER -
ID: 23905901