Research output: Contribution to journal › Article › peer-review
Gold nanoparticles permit in situ absorbed dose evaluation in boron neutron capture therapy for malignant tumors. / Zaboronok, Alexander; Taskaev, Sergey; Volkova, Olga et al.
In: Pharmaceutics, Vol. 13, No. 9, 1490, 16.09.2021.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Gold nanoparticles permit in situ absorbed dose evaluation in boron neutron capture therapy for malignant tumors
AU - Zaboronok, Alexander
AU - Taskaev, Sergey
AU - Volkova, Olga
AU - Mechetina, Ludmila
AU - Kasatova, Anna
AU - Sycheva, Tatiana
AU - Nakai, Kei
AU - Kasatov, Dmitrii
AU - Makarov, Aleksandr
AU - Kolesnikov, Iaroslav
AU - Shchudlo, Ivan
AU - Bykov, Timofey
AU - Sokolova, Evgeniia
AU - Koshkarev, Alexey
AU - Kanygin, Vladimir
AU - Kichigin, Aleksandr
AU - Mathis, Bryan J.
AU - Ishikawa, Eiichi
AU - Matsumura, Akira
N1 - Funding Information: Funding: This work was supported by Grants-in-Aid for Scientific Research (C) JSPS KAKENHI [20K07672] and (B) [18H02909] from the Japanese Ministry of Education, Culture, Sports, Science and Technology. Neutron generation study was supported by the Russian Science Foundation (project no. 19-72-30005). Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2021/9/16
Y1 - 2021/9/16
N2 - Boron neutron capture therapy (BNCT) is an anticancer modality realized through10 B accumulation in tumor cells, neutron irradiation of the tumor, and decay of boron atoms with the release of alpha-particles and lithium nuclei that damage tumor cell DNA. As high-LET particle release takes place inside tumor cells absorbed dose calculations are difficult, since no essential extracellular energy is emitted. We placed gold nanoparticles inside tumor cells saturated with boron to more accurately measure the absorbed dose. T98G cells accumulated ~50 nm gold nanoparticles (AuNPs, 50 µg gold/mL) and boron-phenylalanine (BPA, 10, 20, 40 µg boron-10/mL), and were irradiated with a neutron flux of 3 × 108 cm−2 s−1 . Gamma-rays (411 keV) emitted by AuNPs in the cells were measured by a spectrometer and the absorbed dose was calculated using the formula D = (k × N × n)/m, where D was the absorbed dose (GyE), k—depth-related irradiation coefficient, N—number of activated gold atoms, n—boron concentration (ppm), and m—the mass of gold (g). Cell survival curves were fit to the linear-quadratic (LQ) model. We found no influence from the presence of the AuNPs on BNCT efficiency. Our approach will lead to further development of combined boron and high-Z element-containing compounds, and to further adaptation of isotope scanning for BNCT dosimetry.
AB - Boron neutron capture therapy (BNCT) is an anticancer modality realized through10 B accumulation in tumor cells, neutron irradiation of the tumor, and decay of boron atoms with the release of alpha-particles and lithium nuclei that damage tumor cell DNA. As high-LET particle release takes place inside tumor cells absorbed dose calculations are difficult, since no essential extracellular energy is emitted. We placed gold nanoparticles inside tumor cells saturated with boron to more accurately measure the absorbed dose. T98G cells accumulated ~50 nm gold nanoparticles (AuNPs, 50 µg gold/mL) and boron-phenylalanine (BPA, 10, 20, 40 µg boron-10/mL), and were irradiated with a neutron flux of 3 × 108 cm−2 s−1 . Gamma-rays (411 keV) emitted by AuNPs in the cells were measured by a spectrometer and the absorbed dose was calculated using the formula D = (k × N × n)/m, where D was the absorbed dose (GyE), k—depth-related irradiation coefficient, N—number of activated gold atoms, n—boron concentration (ppm), and m—the mass of gold (g). Cell survival curves were fit to the linear-quadratic (LQ) model. We found no influence from the presence of the AuNPs on BNCT efficiency. Our approach will lead to further development of combined boron and high-Z element-containing compounds, and to further adaptation of isotope scanning for BNCT dosimetry.
KW - Absorbed dose
KW - Accelerator-based neutron source
KW - Boron neutron capture therapy
KW - Dosimetry
KW - Gold nanoparticles
UR - http://www.scopus.com/inward/record.url?scp=85115348647&partnerID=8YFLogxK
U2 - 10.3390/pharmaceutics13091490
DO - 10.3390/pharmaceutics13091490
M3 - Article
C2 - 34575566
AN - SCOPUS:85115348647
VL - 13
JO - Pharmaceutics
JF - Pharmaceutics
SN - 1999-4923
IS - 9
M1 - 1490
ER -
ID: 34268436