Standard

Genome editing system CRISPR/CAS9 and peculiarities of its application in monocots. / Gerasimova, S. V.; Khlestkina, E. K.; Kochetov, A. V. et al.

In: Russian Journal of Plant Physiology, Vol. 64, No. 2, 01.03.2017, p. 141-155.

Research output: Contribution to journal › Review article › peer-review

Harvard

Gerasimova, SV, Khlestkina, EK, Kochetov, AV & Shumny, VK 2017, 'Genome editing system CRISPR/CAS9 and peculiarities of its application in monocots', Russian Journal of Plant Physiology, vol. 64, no. 2, pp. 141-155. https://doi.org/10.1134/S1021443717010071

APA

Gerasimova, S. V., Khlestkina, E. K., Kochetov, A. V., & Shumny, V. K. (2017). Genome editing system CRISPR/CAS9 and peculiarities of its application in monocots. Russian Journal of Plant Physiology, 64(2), 141-155. https://doi.org/10.1134/S1021443717010071

Vancouver

Gerasimova SV, Khlestkina EK, Kochetov AV, Shumny VK. Genome editing system CRISPR/CAS9 and peculiarities of its application in monocots. Russian Journal of Plant Physiology. 2017 Mar 1;64(2):141-155. doi: 10.1134/S1021443717010071

Author

Gerasimova, S. V. ; Khlestkina, E. K. ; Kochetov, A. V. et al. / Genome editing system CRISPR/CAS9 and peculiarities of its application in monocots. In: Russian Journal of Plant Physiology. 2017 ; Vol. 64, No. 2. pp. 141-155.

BibTeX

@article{dd9ad7f789fe4da6a19b9109e28d676a,
title = "Genome editing system CRISPR/CAS9 and peculiarities of its application in monocots",
abstract = "Genome editing is a new methodology for DNA modification that has been developing in recent years. This review compares proposed methods of optimization and development of a modern genome editing system—CRISPR/Cas9—in monocots. Methodical approaches for in silico selecting target sites, designing an expression vector, transferring the vector expression cassette into plant cells, evaluating the results of the editing and nonspecific activity of the system, and obtaining modified plants free of foreign DNA are reviewed. The problem of legislative regulation and the prospects for using this method for commercial purposes are discussed.",
keywords = "cereals, CRISPR/Cas9, crops, gene knockout, genome editing, genome engineering, GMOs, transgenic plants",
author = "Gerasimova, {S. V.} and Khlestkina, {E. K.} and Kochetov, {A. V.} and Shumny, {V. K.}",
year = "2017",
month = mar,
day = "1",
doi = "10.1134/S1021443717010071",
language = "English",
volume = "64",
pages = "141--155",
journal = "Russian Journal of Plant Physiology",
issn = "1021-4437",
publisher = "Maik Nauka-Interperiodica Publishing",
number = "2",

}

RIS

TY - JOUR

T1 - Genome editing system CRISPR/CAS9 and peculiarities of its application in monocots

AU - Gerasimova, S. V.

AU - Khlestkina, E. K.

AU - Kochetov, A. V.

AU - Shumny, V. K.

PY - 2017/3/1

Y1 - 2017/3/1

N2 - Genome editing is a new methodology for DNA modification that has been developing in recent years. This review compares proposed methods of optimization and development of a modern genome editing system—CRISPR/Cas9—in monocots. Methodical approaches for in silico selecting target sites, designing an expression vector, transferring the vector expression cassette into plant cells, evaluating the results of the editing and nonspecific activity of the system, and obtaining modified plants free of foreign DNA are reviewed. The problem of legislative regulation and the prospects for using this method for commercial purposes are discussed.

AB - Genome editing is a new methodology for DNA modification that has been developing in recent years. This review compares proposed methods of optimization and development of a modern genome editing system—CRISPR/Cas9—in monocots. Methodical approaches for in silico selecting target sites, designing an expression vector, transferring the vector expression cassette into plant cells, evaluating the results of the editing and nonspecific activity of the system, and obtaining modified plants free of foreign DNA are reviewed. The problem of legislative regulation and the prospects for using this method for commercial purposes are discussed.

KW - cereals

KW - CRISPR/Cas9

KW - crops

KW - gene knockout

KW - genome editing

KW - genome engineering

KW - GMOs

KW - transgenic plants

UR - http://www.scopus.com/inward/record.url?scp=85014566647&partnerID=8YFLogxK

U2 - 10.1134/S1021443717010071

DO - 10.1134/S1021443717010071

M3 - Review article

AN - SCOPUS:85014566647

VL - 64

SP - 141

EP - 155

JO - Russian Journal of Plant Physiology

JF - Russian Journal of Plant Physiology

SN - 1021-4437

IS - 2

ER -

ID: 10064138