Fluorescent labeling of ursolic acid with FITC for investigation of its cytotoxic activity using confocal microscopy

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Fluorescent labeling of ursolic acid with FITC for investigation of its cytotoxic activity using confocal microscopy. / Frolova, Tatiana S.; Lipeeva, Alla V.; Baev, Dmitry S. et al.

In: Bioorganic Chemistry, Vol. 87, 01.06.2019, p. 876-887.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{31130d6f9d0d460db97c97b6e1eb527e,

title = "Fluorescent labeling of ursolic acid with FITC for investigation of its cytotoxic activity using confocal microscopy",

abstract = "Fluorescent labeling is a widely-used approach in the study of intracellular processes. This method is becoming increasingly popular for studying small bioactive molecules of natural origin; it allows us to estimate the vital intracellular changes which occur under their influence. We propose a new approach for visualization of the intracellular distribution of triterpene acids, based on fluorescent labeling by fluoresceine isothiocyanate. As a model compound we took the most widely-used and best-studied acid in the ursane series – ursolic acid, as this enabled us to compare the results obtained during our research with the available data, in order to evaluate the validity of the proposed method. Experimental tracing of the dynamics of penetration and distribution of the labeled ursolic acid has shown that when the acid enters the cell, it initially localizes on the inner membranes where the predicted target Akt1/protein kinase B – a protein that inhibits apoptosis – is located.",

keywords = "Akt1/protein kinase B, Cytotoxicity, Fluorescence, MDM2/E3 ubiquitin-protein ligase, Triterpene acids, Ursolic acid, APOPTOSIS, LOCALIZATION, ANGIOGENIC ACTIVITY, TUMOR, CANCER, CELL-DEATH, ANTICANCER, PATHWAY, FORCE-FIELD, DERIVATIVES",

author = "Frolova, {Tatiana S.} and Lipeeva, {Alla V.} and Baev, {Dmitry S.} and Baiborodin, {Sergey I.} and Orishchenko, {Кonstantin E.} and Kochetov, {Alexey V.} and Sinitsyna, {Olga I.}",

year = "2019",

month = jun,

day = "1",

doi = "10.1016/j.bioorg.2018.11.052",

language = "English",

volume = "87",

pages = "876--887",

journal = "Bioorganic Chemistry",

issn = "0045-2068",

publisher = "Academic Press Inc.",

}

RIS

TY - JOUR

T1 - Fluorescent labeling of ursolic acid with FITC for investigation of its cytotoxic activity using confocal microscopy

AU - Frolova, Tatiana S.

AU - Lipeeva, Alla V.

AU - Baev, Dmitry S.

AU - Baiborodin, Sergey I.

AU - Orishchenko, Кonstantin E.

AU - Kochetov, Alexey V.

AU - Sinitsyna, Olga I.

PY - 2019/6/1

Y1 - 2019/6/1

N2 - Fluorescent labeling is a widely-used approach in the study of intracellular processes. This method is becoming increasingly popular for studying small bioactive molecules of natural origin; it allows us to estimate the vital intracellular changes which occur under their influence. We propose a new approach for visualization of the intracellular distribution of triterpene acids, based on fluorescent labeling by fluoresceine isothiocyanate. As a model compound we took the most widely-used and best-studied acid in the ursane series – ursolic acid, as this enabled us to compare the results obtained during our research with the available data, in order to evaluate the validity of the proposed method. Experimental tracing of the dynamics of penetration and distribution of the labeled ursolic acid has shown that when the acid enters the cell, it initially localizes on the inner membranes where the predicted target Akt1/protein kinase B – a protein that inhibits apoptosis – is located.

AB - Fluorescent labeling is a widely-used approach in the study of intracellular processes. This method is becoming increasingly popular for studying small bioactive molecules of natural origin; it allows us to estimate the vital intracellular changes which occur under their influence. We propose a new approach for visualization of the intracellular distribution of triterpene acids, based on fluorescent labeling by fluoresceine isothiocyanate. As a model compound we took the most widely-used and best-studied acid in the ursane series – ursolic acid, as this enabled us to compare the results obtained during our research with the available data, in order to evaluate the validity of the proposed method. Experimental tracing of the dynamics of penetration and distribution of the labeled ursolic acid has shown that when the acid enters the cell, it initially localizes on the inner membranes where the predicted target Akt1/protein kinase B – a protein that inhibits apoptosis – is located.

KW - Akt1/protein kinase B

KW - Cytotoxicity

KW - Fluorescence

KW - MDM2/E3 ubiquitin-protein ligase

KW - Triterpene acids

KW - Ursolic acid

KW - APOPTOSIS

KW - LOCALIZATION

KW - ANGIOGENIC ACTIVITY

KW - TUMOR

KW - CANCER

KW - CELL-DEATH

KW - ANTICANCER

KW - PATHWAY

KW - FORCE-FIELD

KW - DERIVATIVES

UR - http://www.scopus.com/inward/record.url?scp=85057730940&partnerID=8YFLogxK

U2 - 10.1016/j.bioorg.2018.11.052

DO - 10.1016/j.bioorg.2018.11.052

M3 - Article

C2 - 30538052

AN - SCOPUS:85057730940

VL - 87

SP - 876

EP - 887

JO - Bioorganic Chemistry

JF - Bioorganic Chemistry

SN - 0045-2068

ER -

ID: 18185492