Research output: Contribution to journal › Article › peer-review
First report on B chromosome content in a reptilian species : the case of Anolis carolinensis. / Kichigin, Ilya G.; Lisachov, Artem P.; Giovannotti, Massimo et al.
In: Molecular Genetics and Genomics, Vol. 294, No. 1, 11.02.2019, p. 13-21.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - First report on B chromosome content in a reptilian species
T2 - the case of Anolis carolinensis
AU - Kichigin, Ilya G.
AU - Lisachov, Artem P.
AU - Giovannotti, Massimo
AU - Makunin, Alex I.
AU - Kabilov, Marsel R.
AU - O’Brien, Patricia C.M.
AU - Ferguson-Smith, Malcolm A.
AU - Graphodatsky, Alexander S.
AU - Trifonov, Vladimir A.
PY - 2019/2/11
Y1 - 2019/2/11
N2 - Supernumerary elements of the genome are often called B chromosomes. They usually consist of various autosomal sequences and, because of low selective pressure, are mostly pseudogenized and contain many repeats. There are numerous reports on B chromosomes in mammals, fish, invertebrates, plants, and fungi, but only a few of them have been studied using sequencing techniques. However, reptilian supernumerary chromosomes have been detected only cytogenetically and never sequenced or analyzed at the molecular level. One model squamate species with available genome sequence is Anolis carolinensis. The scope of the present article is to describe the genetic content of A. carolinensis supernumerary chromosomes. In this article, we confirm the presence of B chromosomes in this species by reverse painting and synaptonemal complex analysis. We applied low-pass high-throughput sequencing to analyze flow-sorted B chromosomes. Anole B chromosomes exhibit similar traits to other supernumerary chromosomes from different taxons: they contain two genes related to cell division control (INCENP and SPIRE2), are enriched in specific repeats, and show a high degree of pseudogenization. Therefore, the present study confirms that reptilian B chromosomes resemble supernumerary chromosomes of other taxons.
AB - Supernumerary elements of the genome are often called B chromosomes. They usually consist of various autosomal sequences and, because of low selective pressure, are mostly pseudogenized and contain many repeats. There are numerous reports on B chromosomes in mammals, fish, invertebrates, plants, and fungi, but only a few of them have been studied using sequencing techniques. However, reptilian supernumerary chromosomes have been detected only cytogenetically and never sequenced or analyzed at the molecular level. One model squamate species with available genome sequence is Anolis carolinensis. The scope of the present article is to describe the genetic content of A. carolinensis supernumerary chromosomes. In this article, we confirm the presence of B chromosomes in this species by reverse painting and synaptonemal complex analysis. We applied low-pass high-throughput sequencing to analyze flow-sorted B chromosomes. Anole B chromosomes exhibit similar traits to other supernumerary chromosomes from different taxons: they contain two genes related to cell division control (INCENP and SPIRE2), are enriched in specific repeats, and show a high degree of pseudogenization. Therefore, the present study confirms that reptilian B chromosomes resemble supernumerary chromosomes of other taxons.
KW - Carolina anole
KW - DOP-PCR
KW - High-throughput sequencing
KW - Squamata
KW - Supernumerary chromosomes
KW - LIZARD
KW - SEX-CHROMOSOMES
KW - RED FOX
KW - GENOME
KW - ORIGIN
KW - EVOLUTION
KW - SQUAMATA
KW - DNA
KW - NYCTEREUTES-PROCYONOIDES
KW - DOG
UR - http://www.scopus.com/inward/record.url?scp=85052304735&partnerID=8YFLogxK
U2 - 10.1007/s00438-018-1483-9
DO - 10.1007/s00438-018-1483-9
M3 - Article
C2 - 30146671
AN - SCOPUS:85052304735
VL - 294
SP - 13
EP - 21
JO - Molecular Genetics and Genomics
JF - Molecular Genetics and Genomics
SN - 1617-4615
IS - 1
ER -
ID: 16239374