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Features of SARS-CoV-2 Replication in Various Types of Reptilian and Fish Cell Cultures. / Kononova, Yulia; Adamenko, Lyubov; Kazachkova, Evgeniya et al.

In: Viruses, Vol. 15, No. 12, 2350, 12.2023.

Research output: Contribution to journal › Article › peer-review

Harvard

Kononova, Y, Adamenko, L, Kazachkova, E, Solomatina, M, Romanenko, S, Proskuryakova, A, Utkin, Y, Gulyaeva, M, Spirina, A, Kazachinskaia, E, Palyanova, N, Mishchenko, O, Chepurnov, A & Shestopalov, A 2023, 'Features of SARS-CoV-2 Replication in Various Types of Reptilian and Fish Cell Cultures', Viruses, vol. 15, no. 12, 2350. https://doi.org/10.3390/v15122350

APA

Kononova, Y., Adamenko, L., Kazachkova, E., Solomatina, M., Romanenko, S., Proskuryakova, A., Utkin, Y., Gulyaeva, M., Spirina, A., Kazachinskaia, E., Palyanova, N., Mishchenko, O., Chepurnov, A., & Shestopalov, A. (2023). Features of SARS-CoV-2 Replication in Various Types of Reptilian and Fish Cell Cultures. Viruses, 15(12), [2350]. https://doi.org/10.3390/v15122350

Vancouver

Kononova Y, Adamenko L, Kazachkova E, Solomatina M, Romanenko S, Proskuryakova A et al. Features of SARS-CoV-2 Replication in Various Types of Reptilian and Fish Cell Cultures. Viruses. 2023 Dec;15(12):2350. doi: 10.3390/v15122350

Author

Kononova, Yulia ; Adamenko, Lyubov ; Kazachkova, Evgeniya et al. / Features of SARS-CoV-2 Replication in Various Types of Reptilian and Fish Cell Cultures. In: Viruses. 2023 ; Vol. 15, No. 12.

BibTeX

@article{8bf2a785526049168a67e36506f1a7f4,
title = "Features of SARS-CoV-2 Replication in Various Types of Reptilian and Fish Cell Cultures",
abstract = "BACKGROUND: SARS-CoV-2 can enter the environment from the feces of COVID-19 patients and virus carriers through untreated sewage. The virus has shown the ability to adapt to a wide range of hosts, so the question of the possible involvement of aquafauna and animals of coastal ecosystems in maintaining its circulation remains open.METHODS: the aim of this work was to study the tropism of SARS-CoV-2 for cells of freshwater fish and reptiles, including those associated with aquatic and coastal ecosystems, and the effect of ambient temperature on this process. In a continuous cell culture FHM (fathead minnow) and diploid fibroblasts CGIB (silver carp), SARS-CoV-2 replication was not maintained at either 25 °C or 29 °C. At 29 °C, the continuous cell culture TH-1 (eastern box turtle) showed high susceptibility to SARS-CoV-2, comparable to Vero E6 (development of virus-induced cytopathic effect (CPE) and an infectious titer of 7.5 ± 0.17 log10 TCID50/mL on day 3 after infection), and primary fibroblasts CNI (Nile crocodile embryo) showed moderate susceptibility (no CPE, infectious titer 4.52 ± 0.14 log10 TCID50/mL on day 5 after infection). At 25 °C, SARS-CoV-2 infection did not develop in TH-1 and CNI.CONCLUSIONS: our results show the ability of SARS-CoV-2 to effectively replicate without adaptation in the cells of certain reptile species when the ambient temperature rises.",
keywords = "Animals, Chlorocebus aethiops, Humans, SARS-CoV-2, Vero Cells, COVID-19, Ecosystem, Cell Culture Techniques",
author = "Yulia Kononova and Lyubov Adamenko and Evgeniya Kazachkova and Mariya Solomatina and Svetlana Romanenko and Anastasia Proskuryakova and Yaroslav Utkin and Marina Gulyaeva and Anastasia Spirina and Elena Kazachinskaia and Natalia Palyanova and Oksana Mishchenko and Alexander Chepurnov and Alexander Shestopalov",
note = "This work was supported by the Russian Science Foundation grant 23-64-00005.",
year = "2023",
month = dec,
doi = "10.3390/v15122350",
language = "English",
volume = "15",
journal = "Viruses",
issn = "1999-4915",
publisher = "Multidisciplinary Digital Publishing Institute (MDPI)",
number = "12",

}

RIS

TY - JOUR

T1 - Features of SARS-CoV-2 Replication in Various Types of Reptilian and Fish Cell Cultures

AU - Kononova, Yulia

AU - Adamenko, Lyubov

AU - Kazachkova, Evgeniya

AU - Solomatina, Mariya

AU - Romanenko, Svetlana

AU - Proskuryakova, Anastasia

AU - Utkin, Yaroslav

AU - Gulyaeva, Marina

AU - Spirina, Anastasia

AU - Kazachinskaia, Elena

AU - Palyanova, Natalia

AU - Mishchenko, Oksana

AU - Chepurnov, Alexander

AU - Shestopalov, Alexander

N1 - This work was supported by the Russian Science Foundation grant 23-64-00005.

PY - 2023/12

Y1 - 2023/12

N2 - BACKGROUND: SARS-CoV-2 can enter the environment from the feces of COVID-19 patients and virus carriers through untreated sewage. The virus has shown the ability to adapt to a wide range of hosts, so the question of the possible involvement of aquafauna and animals of coastal ecosystems in maintaining its circulation remains open.METHODS: the aim of this work was to study the tropism of SARS-CoV-2 for cells of freshwater fish and reptiles, including those associated with aquatic and coastal ecosystems, and the effect of ambient temperature on this process. In a continuous cell culture FHM (fathead minnow) and diploid fibroblasts CGIB (silver carp), SARS-CoV-2 replication was not maintained at either 25 °C or 29 °C. At 29 °C, the continuous cell culture TH-1 (eastern box turtle) showed high susceptibility to SARS-CoV-2, comparable to Vero E6 (development of virus-induced cytopathic effect (CPE) and an infectious titer of 7.5 ± 0.17 log10 TCID50/mL on day 3 after infection), and primary fibroblasts CNI (Nile crocodile embryo) showed moderate susceptibility (no CPE, infectious titer 4.52 ± 0.14 log10 TCID50/mL on day 5 after infection). At 25 °C, SARS-CoV-2 infection did not develop in TH-1 and CNI.CONCLUSIONS: our results show the ability of SARS-CoV-2 to effectively replicate without adaptation in the cells of certain reptile species when the ambient temperature rises.

AB - BACKGROUND: SARS-CoV-2 can enter the environment from the feces of COVID-19 patients and virus carriers through untreated sewage. The virus has shown the ability to adapt to a wide range of hosts, so the question of the possible involvement of aquafauna and animals of coastal ecosystems in maintaining its circulation remains open.METHODS: the aim of this work was to study the tropism of SARS-CoV-2 for cells of freshwater fish and reptiles, including those associated with aquatic and coastal ecosystems, and the effect of ambient temperature on this process. In a continuous cell culture FHM (fathead minnow) and diploid fibroblasts CGIB (silver carp), SARS-CoV-2 replication was not maintained at either 25 °C or 29 °C. At 29 °C, the continuous cell culture TH-1 (eastern box turtle) showed high susceptibility to SARS-CoV-2, comparable to Vero E6 (development of virus-induced cytopathic effect (CPE) and an infectious titer of 7.5 ± 0.17 log10 TCID50/mL on day 3 after infection), and primary fibroblasts CNI (Nile crocodile embryo) showed moderate susceptibility (no CPE, infectious titer 4.52 ± 0.14 log10 TCID50/mL on day 5 after infection). At 25 °C, SARS-CoV-2 infection did not develop in TH-1 and CNI.CONCLUSIONS: our results show the ability of SARS-CoV-2 to effectively replicate without adaptation in the cells of certain reptile species when the ambient temperature rises.

KW - Animals

KW - Chlorocebus aethiops

KW - Humans

KW - SARS-CoV-2

KW - Vero Cells

KW - COVID-19

KW - Ecosystem

KW - Cell Culture Techniques

UR - https://www.scopus.com/record/display.uri?eid=2-s2.0-85180432468&origin=inward&txGid=6dcd98e316eee3a31c3d34691a362475

U2 - 10.3390/v15122350

DO - 10.3390/v15122350

M3 - Article

C2 - 38140591

VL - 15

JO - Viruses

JF - Viruses

SN - 1999-4915

IS - 12

M1 - 2350

ER -

ID: 59535437