TY - JOUR

T1 - Features of emotional and social behavioral phenotypes of calsyntenin2 knockout mice

AU - Ranneva, S. V.

AU - Pavlov, K. S.

AU - Gromova, A. V.

AU - Amstislavskaya, T. G.

AU - Lipina, T. V.

N1 - Copyright © 2017 Elsevier B.V. All rights reserved.

PY - 2017/8/14

Y1 - 2017/8/14

N2 - Calsyntenin-2 (Clstn2) is the synaptic protein that belongs to the super family of cadherins, playing an important role in learning and memory. We recently reported that Clstn2 knockout mice (Clstn2-KO) have a deficit of GABAergic interneurons coupled with hyperactivity and deficient spatial memory. Given, that impaired functioning of GABA receptors is linked to several psychopathologies, including anxiety and autism, we sought to further characterize Clstn2-KO mice with respect to emotional and social behavior. Clstn2-KO males and females were tested in the elevated plus-maze (EPM), open field (OF), forced swim test, social affiliation and recognition test, social transmission of food preference (STFP), dyadic social interactions and marble burying test. Clstn2-KO mice demonstrated high exploration and hyperactivity in the dimly lit EPM that affect anxiety parameters. In contrast, in a more adverse situation in the OF have increased emotionality in Clstn2-KO males, not females. Assessment of hyperactivity for prolong period in the OF showed that Clstn2-KO animals were able to decline their hyperactivity, but their ambulation still remained higher than in WT littermates. Additionally, Clstn2-KO mice expressed stereotyped behavior. Strikingly, analysis of social behavior identified deficient social motivation and social recognition only in Clstn2-KO males, but not in females. Further analysis of social communication in the STFP and direct observation of agonistic interactions confirmed the reduced social behavior in Clstn2-KO males. Altogether, current results showed Clstn2 gene and sex interactions on socio-emotional performance in mice, suggesting a possible role of calsyntenin2 in psychopathological mechanisms of autism.

AB - Calsyntenin-2 (Clstn2) is the synaptic protein that belongs to the super family of cadherins, playing an important role in learning and memory. We recently reported that Clstn2 knockout mice (Clstn2-KO) have a deficit of GABAergic interneurons coupled with hyperactivity and deficient spatial memory. Given, that impaired functioning of GABA receptors is linked to several psychopathologies, including anxiety and autism, we sought to further characterize Clstn2-KO mice with respect to emotional and social behavior. Clstn2-KO males and females were tested in the elevated plus-maze (EPM), open field (OF), forced swim test, social affiliation and recognition test, social transmission of food preference (STFP), dyadic social interactions and marble burying test. Clstn2-KO mice demonstrated high exploration and hyperactivity in the dimly lit EPM that affect anxiety parameters. In contrast, in a more adverse situation in the OF have increased emotionality in Clstn2-KO males, not females. Assessment of hyperactivity for prolong period in the OF showed that Clstn2-KO animals were able to decline their hyperactivity, but their ambulation still remained higher than in WT littermates. Additionally, Clstn2-KO mice expressed stereotyped behavior. Strikingly, analysis of social behavior identified deficient social motivation and social recognition only in Clstn2-KO males, but not in females. Further analysis of social communication in the STFP and direct observation of agonistic interactions confirmed the reduced social behavior in Clstn2-KO males. Altogether, current results showed Clstn2 gene and sex interactions on socio-emotional performance in mice, suggesting a possible role of calsyntenin2 in psychopathological mechanisms of autism.

KW - Autism

KW - Calsyntenin2

KW - Hyperactivity

KW - Knockout mice

KW - Social behavior

KW - Food Preferences

KW - Membrane Proteins/deficiency

KW - Endophenotypes

KW - Male

KW - Emotions/physiology

KW - Sex Characteristics

KW - Motor Activity

KW - Calcium-Binding Proteins/deficiency

KW - Mice, Knockout

KW - Smell

KW - Learning

KW - Animals

KW - Social Behavior

KW - Autistic Disorder/metabolism

KW - Female

KW - Recognition (Psychology)

UR - http://www.scopus.com/inward/record.url?scp=85021281475&partnerID=8YFLogxK

U2 - 10.1016/j.bbr.2017.06.029

DO - 10.1016/j.bbr.2017.06.029

M3 - Article

C2 - 28647593

AN - SCOPUS:85021281475

VL - 332

SP - 343

EP - 354

JO - Behavioural Brain Research

JF - Behavioural Brain Research

SN - 0166-4328

ER -